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A method for extracting and purifying polysaccharides from Ferulica ferulae

A purification method, the technology of ferulae ferulae, is applied in the field of extraction and purification of biological polysaccharides from ferulae ferulae, extraction and purification of biologically active polysaccharides, and extraction and purification of natural product polysaccharides. The effect of long extraction time and high labor cost

Inactive Publication Date: 2017-02-01
XINJIANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, polyamide chromatography column has not been used in the purification of crude polysaccharides from ferulae ferulae, and related reports and studies have not been seen in China.

Method used

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  • A method for extracting and purifying polysaccharides from Ferulica ferulae

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Embodiment 1

[0027] Example 1: Extraction and Purification of Polysaccharides from Ferulica Ferulae

[0028] The specific preparation method steps of the extraction and purification method of the polysaccharides of ferulica ferulae are as follows:

[0029] (1) Crush and sieve the dried fruiting bodies of Ferulicum ferulae with a pulverizer, weigh 40 mesh Ferula ferulae powder and 80% ethanol according to the ratio of material to liquid 1:10—1:15 (g / ml) at 80°C Reflux for 4h, filter off ethanol, and evaporate the residue to dryness under reduced pressure.

[0030](2) Ultrasonic-microwave extraction: use the response surface optimization method to determine the extraction temperature, extraction time and solid-liquid ratio Treatment process conditions: mix the residue obtained in step (2) with water according to the solid-liquid ratio of 1:15 (g / ml) Afterwards, it was moved into an ultrasonic microwave extractor, and extracted for 15 minutes at an ultrasonic power of 50W, an extraction temp...

Embodiment 2

[0037] (1) Raw material pretreatment: cut the dried fruiting bodies of Ferulae ferulae into pieces, crush them with a pulverizer, pass through a 40-mesh sieve, weigh 10g of Ferulae ferulae powder, add 150ml of 80% ethanol to it and reflux twice at 80°C, each time 2h, ethanol was filtered off, and the residue was evaporated to dryness under reduced pressure.

[0038] (2) Ultrasonic-microwave extraction: add 150ml of water to the pretreated residue, dissolve evenly, and then transfer it to an ultrasonic microwave extractor. Extract for 15 minutes at an ultrasonic power of 70W and an extraction temperature of 70°C, and then extract the filtrate at a speed of 4000r / min. , refrigerated and centrifuged for 15min, after collecting the supernatant, the residue was subjected to ultrasonic microwave extraction again, and the two supernatants were combined, and then 4 times the volume of 95% ethanol was added to the supernatant, so that the volume percentage concentration of ethanol in th...

Embodiment 3

[0043] Embodiment three: comparative example 1

[0044] Extraction of polysaccharides by hot water leaching: cut the dried fruiting bodies of Ferula ferulae into pieces, crush them with a pulverizer, and pass through a 40-mesh sieve. After 2h, ethanol was filtered off, and the residue was evaporated to dryness under reduced pressure. Add 150ml of water to the pretreated residue, dissolve evenly, reflux twice in a water bath at 80°C for 2 hours each time, combine the two extracts, and then concentrate in vacuum with a vacuum degree of -0.07~-0.08MPa and a temperature of ≤70 ℃). Freeze and dry to obtain the crude polysaccharide of feruli mushroom in solid state.

[0045] Sevag method for deproteinization: obtain the solution of crude polysaccharide solid solution, and Sevag reagent (V chloroform: V n-butanol = 4:1) according to the volume ratio of 5:1, shake 3 times for 15 minutes each time, refrigerate centrifuge at 4000r After centrifuging for 15 minutes at a speed of 1 / min...

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Abstract

The invention discloses a method for extracting and purifying fleurotus ferulae polysaccharide. The method comprises the following steps: crushing fleurotus ferulae sporocarps, sieving through a 40-mesh sieve, refluxing at 80 DEG C for 4 hours at a material-water ratio with 80% ethanol of 1:10 to 1:15, filtering to remove ethanol, drying the residues by reduction vaporization, mixing the residues with water at a material-water ratio of 1:15, and carrying out ultrasonic-microwave extracting for 15 minutes at ultrasonic power of 50 W, an extracting temperature of 70 DEG C, microwave power of 2450 MHz and power 70 W; centrifugally filtering the filtrate, carrying out ultrasonic-microwave extracting on the residues again, mixing the filtrate obtained in the two extraction operations, centrifuging, extracting the supernatant, removing impurities, and adsorbing and purifying a crude polysaccharide extracting solution on a polyamide chromatographic column, wherein the use amount of polyamide is 5 times of volume of polysaccharide, the eluant is deionized water twice as large as the column volume, and the elution speed is 1.5 ml / min; and concentrating and vacuum-drying to obtain purified fleurotus ferulae polysaccharide. The method disclosed by the invention is simple in process route, low in energy consumption, and capable of significantly shortening the extraction time and improving the extraction rate, and the product is high in purity, good in biological activity and stable in quality.

Description

technical field [0001] The present invention relates to the technical field of extracting and purifying natural product polysaccharides, specifically, the present invention relates to the technical field of extracting and purifying biologically active polysaccharides, and furthermore, the present invention relates to the technical field of extracting and purifying biological polysaccharides of Ferula ferulae. Background technique [0002] Asafoetida is one of the characteristic biological resources in Xinjiang. It is named for its growth on the arid plant Ferulicum ferulae in Xinjiang grasslands. The fungus polysaccharides of ferulae ferulae can enhance human immunity and regulate human physiological balance, and are very popular among people. , is a natural nutritional health food. [0003] Asafoetida is delicious and nutritious. It is recorded in the prior art that Asafoetida contains 18 kinds of amino acids, and all 8 kinds of essential amino acids are available in the hu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 王亮陈义勇张伟丰丁慧玲许丹丹买吾拉江
Owner XINJIANG UNIVERSITY
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