Scrophularia ningpoensis polysaccharide and method for microwave extraction of scrophularia ningpoensis polysaccharide

An extraction method and technology of Scrophulariaceae, applied in the field of polysaccharides from Scrophulariaceae Scrophulariae and its extraction of polysaccharides from Chinese medicinal material Scrophulariae Scrophulariae with microwave assistance, can solve the problems of extraction solvent consumption, long extraction time, environmental pollution, etc., shorten the extraction time, select Strong performance and the effect of improving economic benefits

Inactive Publication Date: 2015-07-01
JISHOU UNIVERSITY
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Problems solved by technology

At present, the extraction of polysaccharides from Scrophulariaceae Scrophulariae is mostly carried out in single batches of cans, and the yield

Method used

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  • Scrophularia ningpoensis polysaccharide and method for microwave extraction of scrophularia ningpoensis polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Processing of raw materials: Radix Scrophulariae can be wet or dry. If it is wet, it will be beaten to 25 mesh. If it is dry, it will be crushed to 25 mesh. Water and a compound enzyme preparation of 3 / 1000 of the medicinal material will be added for enzymolysis. The enzymatic hydrolysis time 9 hours, enzymatic hydrolysis temperature 45°C;

[0030] The proportioning of the compound enzyme preparation is 52% of the mass fraction of cellulase, 30% of the mass fraction of pectinase, and the rest is xylanase. The average enzyme activity of the compound enzyme preparation is 18000 units;

[0031] (2) Microwave extraction: using water as solvent, the solid-liquid ratio of scrophulariaceae to water is 1:12, extracting polysaccharides from scrophulariaceae by microwave-assisted method, and obtaining the extract after centrifugal filtration;

[0032] During the above extraction process, the extraction tank is vacuumed and broken intermittently, and vacuum pulses are used to ...

Embodiment 2

[0042](1) Processing of raw materials: Radix Scrophulariae can be dry or wet. If it is wet, it will be beaten to a pulp. If it is dry, it will be crushed to 45 mesh. Water and a compound enzyme preparation of 7 / 1000 of the medicinal material will be added for enzymolysis. The enzymatic hydrolysis time 4.5 hours, enzymatic hydrolysis temperature 48°C;

[0043] The proportioning of the compound enzyme preparation is 67% of the mass fraction of cellulase, 18% of the mass fraction of pectinase, and the rest is xylanase. The average enzyme activity of the compound enzyme preparation is 27000 units;

[0044] (2) Microwave extraction: using water as solvent, the solid-liquid ratio of scrophulariaceae to water is 1:91, the microwave-assisted method is used to extract the polysaccharides of scrophulariaceae, and the extract is obtained after centrifugal filtration;

[0045] During the above extraction process, the extraction tank is vacuumed and broken intermittently, and vacuum pulses...

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Abstract

The invention discloses scrophularia ningpoensis polysaccharide and a method for microwave extraction of scrophularia ningpoensis polysaccharide. The method comprises the following steps: (1) pulping or grinding crude medicinal materials, and then enzymatically hydrolyzing the medicinal materials with a complex enzyme preparation; (2) carrying out microwave-assisted extraction at the material-to-liquid ratio of 1 to (10-100) of scrophularia ningpoensis to water, centrifugally filtering so as to obtain an extracting solution, and carrying out intermittent vacuumizing and vacuum breaking on an extracting tank during extracting; (3) loading the scrophularia ningpoensis polysaccharide extracting solution on a macroporous adsorption resin chromatographic column, eluting with de-ionized water and collecting eluate; (4) concentrating the eluate in reduced pressure, adding ethanol, fully stirring and uniformly mixing, and standing by; and (5) freeze-drying. By effectively integrating enzymatic hydrolysis with the complex enzyme preparation, vacuum pulsating, microwave assistance, resin adsorption and other technologies, the method disclosed by the invention can significantly improve the extraction rate and the purity of the scrophularia ningpoensis polysaccharide and can shorten an extraction time, and the method is strong in market competitiveness and broad in application prospect; and the prepared scrophularia ningpoensis polysaccharide has a significant inhibitory effect on cancer cells.

Description

technical field [0001] The invention relates to polysaccharides from Scrophulariaceae Scrophulariae and a method for extracting polysaccharides from Scrophulariaceae Scrophulariae, a Chinese medicinal material, and a method for extracting polysaccharides from Scrophulariaceae Scrophulariae. Background technique [0002] Plant polysaccharides are an important topic in the field of biochemistry, and have become a new research hotspot at home and abroad, among which water-soluble polysaccharides extracted from Chinese herbal medicines are particularly popular. Polysaccharides are polymers that are linked by monosaccharides and have a polymerization degree of more than 100,000. Plant polysaccharides are a component commonly found in plant cell walls and cells. At present, hundreds of polysaccharides have been isolated and extracted from nature. A large number of studies have proved that polysaccharides have unique biological activities, and are the main active substances of many...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张朝晖佘高照张春向士全向冬梅
Owner JISHOU UNIVERSITY
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