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A kind of method extracting unsaturated fatty acid from Asian centipede algae

A technology of unsaturated fatty acids and Asian centipede algae, which is applied in the production of fatty acids, chemical modification of fatty acids, and fat production, etc., can solve the problems of low efficiency and achieve the effect of improving extraction efficiency

Active Publication Date: 2018-11-09
THE FIRST INST OF OCEANOGRAPHY SOA
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting unsaturated fatty acids from Asian centipede algae, thereby solving the problem of low efficiency of the traditional extraction method of seaweed unsaturated fatty acids

Method used

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  • A kind of method extracting unsaturated fatty acid from Asian centipede algae

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Embodiment 1

[0017] A. Rinse the Asian centipede algae, add cellulase (Beijing Shengshi Jiaming Technology Development Co., Ltd., 30000u / g, which contains C1 enzyme, Cx enzyme and β-glucosidase,) according to the ratio of mass ratio 1:100 50 ℃ for 2 hours, drained, dried, and then crushed to obtain centipede algae powder;

[0018] B. Add centipede algae residue powder to petroleum ether and soak for 24 hours, the mass volume ratio of centipede algae residue powder to petroleum ether is 1g:12ml, reflux in a water bath at 45-65°C for 2-4 hours, filter and centrifuge to obtain supernatant;

[0019] C, add 0.1M potassium hydroxide, ethanol saponification solution to the supernatant;

[0020] D. Add 1mol / L hydrochloric acid solution after saponification, adjust the pH value to 3.5-4, and the filtrate after suction filtration is unsaturated fatty acid.

[0021] 2. The detection method of unsaturated fatty acids is as follows:

[0022] A. Dissolve the extracted total unsaturated fat with 2-5ml ...

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Abstract

The invention discloses a method for extracting unsaturated fatty acid from an Asia grateloupia filicina. The method comprises the following steps: washing Asia grateloupia filicina clean; adding a composite cellulase according to the mass ratio of 1:100 and acting at the temperature of 50 DEG C for 2h; draining, drying and smashing to obtain the grateloupia filicina powder; adding the grateloupia filicina powder into petroleum ether, soaking for 24h, performing water bath backflow, filtering and centrifuging to obtain supernate; adding a saponified solution to the supernate, adding a hydrochloric acid solution by the end of saponification, and adjusting the PH value to 3.4-4 to obtain the unsaturated fatty acid. The gas chromatography shows that in the unsaturated fatty acid extracted from the grateloupia filicina pretreated by the composite cellulase, the content of timnodonic acid (C20:5) and eicosatetraenoic acid (C20:4) is greater than 75% of the total unsaturated fatty acid and is about 50% higher than that of the direct extraction process. A method for efficiently extracting the unsaturated fatty acid from the Asia grateloupia filicina is established for the first time and a scientific basis for the efficient comprehensive utilization of Asia grateloupia filicina is provided.

Description

technical field [0001] The invention belongs to the technical field of fatty acid extraction, and in particular relates to a method for efficiently extracting unsaturated fatty acids from centipede algae. Background technique [0002] The research results show that unsaturated fatty acids have important physiological activities such as anti-atherosclerosis, anti-thrombosis, anti-hyperlipidemia, anti-hypertension, and elimination of inflammation, so they are highly valued by people. However, unsaturated fatty acids cannot be synthesized in the human body and can only be supplemented through diet. Marine fish and fish oils are the main commercial sources of unsaturated fatty acids, but their suitability and safety have been questioned, so there is a need to find suitable potential energy sources to replace them. Seaweed has been reported to be lower in fat but higher in unsaturated fatty acids (PUFAs) than terrestrial organisms. Among them, red algae do not contain 22-carbon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/04C11B1/10C11B3/00C11C3/00
CPCC11B1/04C11B1/10C11B3/00C11B3/001C11B3/008C11C3/00
Inventor 李江
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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