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Preparation method of snail protease

A technology of snail protease and snail, applied in the field of preparation of snail protease, can solve the problems of complex process, low purification rate, poor operability, etc., and achieve the effect of simple process, high purification rate and low price

Inactive Publication Date: 2015-07-01
威海圣奥罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problems of complex process, poor operability, low purification rate and high cost in the existing snail protease extraction method, and provides a preparation method of snail protease with simple process, strong operability, high purification rate and low cost

Method used

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  • Preparation method of snail protease

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] like figure 1 Shown:

[0018] (1) Snail selection:

[0019] Choose more than 15 grams of fresh brown cloud agate snails (scientific name: Achatina Fulica).

[0020] (2) Snail cleaning:

[0021] Put the snails in the stocking box and wash them with clean water at a temperature of 18°C ​​to 28°C to wash off the feces and sundries, and carefully pick out the dead snails every time they are cleaned. For the clean water used for flushing, the temperature is preferably 25°C.

[0022] (3) Snail mucus collection:

[0023] Take 50KG cleaned fresh snails, put them on the workbench, wait for the body to stick out of the shell, use a small charging power supply to adjust the no-load voltage > 900 volts, load current < 4 mA, instantly shock the snail’s feet, one touch That is to say, repeat 5 times. After the mucus is secreted by the snail, it is sucked into the beaker with a dropper, and 5000 grams of mucus can be collected from 50 kilograms of snails.

[0024] (4) The first ...

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Abstract

The invention relates to a preparation method of snail protease and solves the technical problem that the existing snail protease extraction method is complex in process, poor in operability, low in extraction rate and high in cost. The preparation method comprises the following steps: selecting fresh and alive achatina fulica; cleaning; collecting mucus; filtering for the first time; centrifugalizing; filtering for the second time; freeze-drying in vacuum; milling glue; and storing. The preparation method provided by the invention is widely applied to preparing snail protease.

Description

technical field [0001] The invention relates to a method for preparing an enzyme, in particular to a method for preparing a snail protease. Background technique [0002] Snail protease is a biologically active protein prepared from the mucus secreted by the foot glands of snails. It is rich in natural allantoin, calcium, glycolic acid, collagen, protein and vitamins, which are essential for skin maintenance. In addition, snail mucus is a natural antibiotic that kills certain bacteria on people's skin and eliminates skin spots and acne because it penetrates the third layer of the skin. The mucus secreted by snails contains chemical components that have a skin-tightening effect and can quickly regenerate the skin. Allantoin extracted from snail mucus can regenerate the skin; protein and vitamins can increase the nutrition of the skin, making the skin more delicate and smooth. However, the current extraction method of snail protease has the defects of complex process, poor op...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/64
CPCC12N9/6405
Inventor 刘斌杨磊
Owner 威海圣奥罗生物科技有限公司