Arsenic induced operon gene and application thereof

An inducible, operon technology, applied in the field of genetically engineered biosensors, can solve the problems of low detection limit and insufficient sensitivity of bacterial biosensors, and achieve the effects of high sensitivity and low background fluorescence

Active Publication Date: 2015-07-01
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide an arsenic-inducible operon gene and its application to solve the problem of low detection limit, insufficient sensitivity, as well as the technical problems of unavoidable background fluorescence

Method used

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  • Arsenic induced operon gene and application thereof
  • Arsenic induced operon gene and application thereof
  • Arsenic induced operon gene and application thereof

Examples

Experimental program
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Embodiment 1

[0028] 1. Test material

[0029] 1) Preparation of LB medium:

[0030] LB liquid medium: 10g / L tryptone, 5g / L yeast extract, 10g / L sodium chloride;

[0031] LB solid medium: add 15g of agar per liter of LB liquid medium;

[0032] Ampicillin-resistant LB solid medium: Heat the prepared LB solid medium to dissolve completely, and add ampicillin with a total weight of 1‰ when the temperature drops to about 55°C.

[0033] 2) Preparation of TB liquid medium: Add 12g of tryptone, 24g of yeast extract and 4mL of glycerin into 0.9L of ultrapure water, stir well to obtain liquid A; at the same time, weigh 2.31 KH 2 PO 4 and 12.54gK 2 HPO 4 Dissolve and dilute in 100mL ultrapure water to obtain liquid B; sterilize liquids A and B separately under high temperature and high pressure, and mix the two after the temperature is cooled to 60°C to obtain 1L of TB liquid medium;

[0034] Ampicillin-resistant TB liquid medium: add 3‰ total weight of ampicillin to the prepared TB liquid medi...

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Abstract

The invention discloses an arsenic induced operon gene and application thereof. The operon gene has a nucleotide sequence represented by SEQ ID NO:1 or a nucleotide sequence complementary with the nucleotide sequence represented by SEQ ID NO:1. An arsenic induced operon is a muton of a wild type arsenic induced operon and can be directly used for optimally constructing an arsenic induced biosensor and detecting the content of arsenic. An arsenic induced bacteria biological sensor constructed based on the operon has the advantages of being high in sensitivity, specific in arsenic detection and low in background fluorescence.

Description

technical field [0001] The invention relates to the technical field of genetic engineering biosensors, in particular to an arsenic-inducible operon gene and its application. Background technique [0002] Heavy metals are one of the main pollutants in the environment, widely distributed in air, soil, water and food, and are not easy to degrade. Except for a small amount of heavy metals such as manganese and zinc which are essential trace elements, most heavy metals such as arsenic and mercury are not required for life activities. When the heavy metals required for these non-life activities enter the human body, they can cause acute or chronic poisoning, leading to inflammation, organ failure and cancer, and finally cause serious harm to human health (Chen, C., et al., Cancer potential in liver, lung, bladder and kidney due to ingested inorganic arsenic in drinking water. British journal of cancer, 1992.66(5):p.888.). Therefore, the detection of heavy metals, especially the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/70C12N15/65C12N1/21C12Q1/68C12Q1/02
Inventor 陈少鹏李鹿之吴李君
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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