A method for increasing the content of cordycepin in the fruiting bodies of Cordyceps militaris
A technology of cordycepin and Cordyceps militaris, which is applied in botany equipment and methods, fertilizer mixtures, gardening, etc., can solve the problems that development and utilization have not been paid attention to by people, and the output of Cordyceps militaris is scarce, so as to achieve good application development prospects and improve Effects on quality, increased yield and efficiency
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Embodiment 1
[0021] S1. Monascus cultivation:
[0022] S11. Take water-soluble starch 4%, sucrose 5%, peptone 4%, agar 5%, sodium carbonate 0.9% and the remaining amount of water, mix them into a conical flask, sterilize, cool, place them on a slope, and inoculate Monascus species, in an incubator at 30°C, cultured in the dark until the mycelium covered the slope;
[0023] S12. Take maltose 24g / L, peptone 34g / L, mannitol 22g / L, sodium nitrate 4g / L, amino acid chelated zinc 4g / L, potassium dihydrogen phosphate 2g / L and mix, put into a conical flask, and use 60mL Sterile water washes the inclined surface of the conical flask obtained in step S11 to obtain a spore suspension, which is 60% of the seed liquid medium by volume, inoculated, and cultured in liquid state on a rotary shaker at 30° C. at 180 r / min for 1 d;
[0024] S13, take starch 18%, sodium nitrate 0.6%, yeast paste 0.8%, corn steep liquor 1% and mix with the remaining amount of water, adjust the pH value with lactic acid to 4.0,...
Embodiment 2
[0030] S1. Monascus cultivation:
[0031] S11. Mix water-soluble starch 2%, sucrose 3%, peptone 3%, agar 1%, sodium carbonate 0.7% with the remaining amount of water and put them into a conical flask, sterilize, cool, place on a slope, and inoculate Monascus strains , in an incubator at 30°C, cultured in the dark until the hyphae are covered with slopes;
[0032] S12. Mix 20g / L of maltose, 30g / L of peptone, 20g / L of mannitol, 2g / L of sodium nitrate, 2g / L of zinc amino acid chelate, and 1g / L of potassium dihydrogen phosphate. Sterile water washes the inclined surface of the conical flask obtained in step S11 to obtain a spore suspension, which is 50% of the seed liquid medium by volume, inoculated, and cultured in liquid state on a rotary shaker at 30° C. at 180 r / min for 1 d;
[0033] S13, take starch 16%, sodium nitrate 0.4%, yeast paste 0.6%, corn steep liquor 0.8% and mix with the remaining amount of water, adjust the pH value with lactic acid to 4.0, put it into a triangu...
Embodiment 3
[0039] S1. Monascus cultivation:
[0040] S11. Take 3% of water-soluble starch, 4% of sucrose, 3.5% of peptone, 3% of agar, 0.8% of sodium carbonate and the remaining amount of water and put it into a triangular flask, sterilized, cooled, placed on an inclined plane, and inoculated with Monascus species, in an incubator at 30°C, cultured in the dark until the mycelium covered the slope;
[0041] S12. Mix 22g / L of maltose, 32g / L of peptone, 21g / L of mannitol, 3g / L of sodium nitrate, 3g / L of zinc amino acid chelate, and 1.5g / L of potassium dihydrogen phosphate, put them into a conical flask, and use 50mL sterile water was used to wash the inclined surface of the conical flask obtained in step S11 to obtain a spore suspension, which was 55% of the seed liquid medium by volume, inoculated, and cultured in liquid state on a rotary shaker at 30° C. at 180 r / min for 1 d ;
[0042] S13, take starch 17%, sodium nitrate 0.5%, yeast paste 0.7%, corn steep liquor 0.9% and mix with the r...
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