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A process for preparing granular threonine product

A technology of threonine and granular threonine, which is applied in the field of preparation of granular threonine products, can solve problems such as unfavorable transportation and storage, environmental pollution, income generation, easy moisture absorption and agglomeration, so as to avoid waste of water resources and improve the cracking rate , low cost effect

Active Publication Date: 2017-08-01
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many defects in the traditional process: first, the traditional powdered threonine, due to its small particle size, is easy to absorb moisture and agglomerate, which is not conducive to transportation and storage, and in the process of adding feed, it has poor fluidity and dust Large, not conducive to adding; second, the fermentation process produces a large amount of bacterial protein and waste water, and its improper use will cause environmental pollution and cannot bring income to the enterprise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Taking Hulunbeier Northeast Fufeng Biotechnology Co., Ltd. with an annual output of 30,000 tons of granular threonine as an example, a process for preparing granular threonine products includes the following steps:

[0023] 1) Preparation of threonine fermentation broth: put Brevibacterium flavum ATCC14067 into the seed tank culture medium according to the inoculation amount of 10% for cultivation, at a temperature of 30°C, with a shaker speed of 150r / min, and cultivate for 24 hours to obtain a seed liquor; Then according to the seed liquid: the volume ratio of the fermenter medium is 1: 12 and is transferred to the fermentor for cultivation, at a temperature of 30°C, cultivated for 96h to obtain a fermented liquid; the basic parameters of the fermented liquid are: pH value 6-8; threonine Content: 9-12g / 100ml; dry matter: 12-15g / 100ml;

[0024] The concentration of the Brevibacterium flavum was 1×10 8 a / mL;

[0025] The medium components of the seed tank are (1L): glu...

Embodiment 2

[0033] In the present invention, the thalline protein prepared in Example 1, whose purity can reach more than 90%, replaces the yeast powder in the commercially available standard YPD medium, and the remaining components remain unchanged. Saccharomyces cerevisiae and Pichia pastoris are cultivated under the same conditions, and by comparison The growth of the cells (OD600) evaluates the culture effect of the product. See Table 1 for details:

[0034] Table 1

[0035] group Saccharomyces cerevisiae cultured for 24 hours Pichia pastoris cultured for 24 hours Example 1 2.43 1.69 commercial yeast powder 2.11 1.55

[0036] Conclusion: Determination of OD by nephelometric method 600 Characterizing the growth of the cells shows that this product can replace the commercially available yeast powder products, the cultivation effect is better, and it can bring huge economic benefits to the enterprise.

Embodiment 3

[0038] Feed breeding test prepared by embodiment 1:

[0039] The feed that embodiment 1 prepares is for the influence of chicken growth and development

[0040] Control group: basal diet: ordinary feed = 8:1;

[0041] Test group: basal diet: Example 1 feed = 8:1;

[0042] A total of 60 healthy chicks were selected and randomly divided into two groups, including a control group and a test group, with 30 chicks in each group, and the test time was 40 days.

[0043] Weighed on the 1st day and the 40th day respectively, and calculated the average daily weight gain (g / bird) of the chickens in each group after being fed with the feed, as shown in Table 2 for details.

[0044] Table 2 Comparison table of average daily weight gain of chickens

[0045] group Average weight on the first day (g / bird) Average weight on the 40th day (g / bird) Average weight gain (g / bird) control group 42.6 1427.3 1384.7 test group 42.9 1628.1 1585.2

[0046] Conclusion...

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Abstract

The invention belongs to the technical field of production of amino acids and in particular relates to a process for preparing a granular type threonine product. The process comprises the following steps: (1) preparing threonine fermentation liquor; (2) preparing powdery threonine; (3) preparing threonine granules; (4) refining a mycoprotein; (5) preparing the feed; and (6) preparing an organic fertilizer. According to the process disclosed by the invention, the threonine granules are prepared, moisture absorption and agglomeration can be avoided, the components of the waste mother solution are effectively utilized, wastes are not produced in the whole process, the process is environmentally friendly and pollution-free, and energy conservation and emission reduction are realized.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a process for preparing granular threonine products. Background technique [0002] Threonine is an essential amino acid, which is the second limiting amino acid in pig feed and the third limiting amino acid in poultry feed. With the wide application of lysine and methionine synthetic products in compound feed, threonine has gradually become an The main limiting factor for the growth of livestock and poultry. There are many defects in the traditional process: first, the traditional powdered threonine, due to its small particle size, is easy to absorb moisture and agglomerate, which is not conducive to transportation and storage, and in the process of adding feed, it has poor fluidity and dust Second, the fermentation process produces a large amount of bacterial protein and waste water, and its improper use will cause environmental pollution and cannot br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/142C05G1/00
Inventor 严纪文赵凤良汲广习白红兵
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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