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Double-functional nano-composite spheres based on metal ion-inducing polypeptide self-assembly and preparation method thereof

A nano-composite, metal ion technology, applied in the direction of drug combinations, preparations for in vivo tests, non-active ingredients of polymer compounds, etc., can solve the problems of difficult diagnosis and detection of cancer, delay the best treatment period, etc., and achieve synthesis cost Inexpensive, simple steps, and broad application prospects

Active Publication Date: 2015-07-08
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In recent years, diseases such as cancer have become more and more serious threats to human health, and cancer is difficult to be diagnosed in the early stage, thus delaying the best treatment period

Method used

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  • Double-functional nano-composite spheres based on metal ion-inducing polypeptide self-assembly and preparation method thereof
  • Double-functional nano-composite spheres based on metal ion-inducing polypeptide self-assembly and preparation method thereof
  • Double-functional nano-composite spheres based on metal ion-inducing polypeptide self-assembly and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Dissolve 0.5 mg of the polypeptide solid obtained by hydrolyzing α-lactalbumin under the action of serine protease in 1 mL of tris solution with a concentration of 75 mM and a pH of 7.5, and shake to dissolve completely; The pH value of aminomethane solution is adjusted with hydrochloric acid;

[0025] (2) Add 120 μL of Bengal Red solution with a concentration of 1 mM to the solution in step (1), mix and shake well;

[0026] (3) 100 μL of GdCl with a concentration of 10 mM 3 solution, and 40 μL of 10 mM CaCl 2 The solution is added to the solution in step (2), and then incubated with shaking at 37°C for 24 hours;

[0027] (4) After the reaction, the solid was collected by centrifugation, which was a bifunctional nanocomposite sphere based on metal ion-induced polypeptide self-assembly, and finally dispersed in 0.5 mL of water.

[0028] Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride EDC of 1.05mg and the N-hydroxysuccinimide of 0.15mg in the dispe...

Embodiment 2

[0030] (1) Dissolve 0.5 mg of a polypeptide solid obtained by hydrolyzing α-lactalbumin under the action of serine protease in 1 mL of a tris solution with a concentration of 75 mM and a pH of 6.5, and shake to dissolve completely; The pH value of aminomethane solution is adjusted with hydrochloric acid;

[0031] (2) Add 120 μL of Bengal Red solution with a concentration of 1 mM to the solution in step (1), mix and shake well; (3) add 40 μL of GdCl with a concentration of 10 mM 3 solution, and 40 μL of 10 mM CaCl 2 The solution is added to the solution in step (2), and then incubated with shaking at 37°C for 24 hours;

[0032] (4) After the reaction, the solid was collected by centrifugation, which was a bifunctional nanocomposite sphere based on metal ion-induced polypeptide self-assembly, and finally dispersed in 2 mL of water.

[0033] Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride EDC of 1.05mg and the N-hydroxysuccinimide of 0.15mg in the dispersion liq...

Embodiment 3

[0035](1) Dissolve 0.5 mg of the polypeptide solid obtained by hydrolyzing α-lactalbumin under the action of serine protease in 1 mL of tris solution with a concentration of 75 mM and a pH of 7.5, and shake to dissolve completely; The pH value of aminomethane solution is adjusted with hydrochloric acid;

[0036] (2) Add 120 μL of Bengal Red solution with a concentration of 1 mM to the solution in step (1), mix and shake well;

[0037] (3) 40 μL of GdCl with a concentration of 10 mM 3 solution, and 50 μL of 10 mM CaCl 2 The solution is added to the solution in step (2), and then incubated with shaking at 37°C for 24 hours;

[0038] (4) After the reaction, the solid was collected by centrifugation, that is, the bifunctional nanocomposite sphere based on metal ion-induced polypeptide self-assembly, and finally dispersed in 1 mL of water.

[0039] Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride EDC of 1.05mg and the N-hydroxysuccinimide of 0.15mg in the dispersi...

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Abstract

The invention discloses a double-functional nano-composite spheres based on metal ion-inducing polypeptide self-assembly and a preparation method thereof. In the invention, by means of a one-step reaction synthesis method, with [alpha]-lactalbumin hydrolyzed polypeptide as a carrier, the nano spheres are self-assembled under induction by Ca<2+> and Gd<3+> together, wherein the nano spheres, during self-assembly, are wrapped by a photosensitive agent, rose Bengal. The particle size of the nano-composite spheres can be adjusted and controlled in the range of 100-500 nm. The Gd<3+> which participates in the inductive sphere forming is used as a contrast medium unit of magnetic resonance imaging and the T1-weighted nuclear magnetic resonance signal response can be effectively increased so that the magnetic resonance imaging diagnosis to a tumor site can be performed. The rose Bengal wrapping the nano-composite spheres can release singlet oxygen to kill tumor cells under excitation illumination at 550 nm, thereby achieving the effect of photodynamic treatment. The nano-composite spheres are toxic-free without illumination. Meanwhile, the treatment process allows tracing through the magnetic resonance imaging. In addition, the nano-composite spheres are low in preparation cost, are simple in processes, and have a potential wide application prospect in the bio-medicinal field.

Description

technical field [0001] The invention belongs to the technical field of preparation of nanocomposite materials, and in particular relates to the design and preparation method of a bifunctional nanocomposite ball based on metal ion-induced polypeptide self-assembly. technical background [0002] In recent years, diseases such as cancer have become more and more serious threats to human health, and cancer is difficult to be diagnosed in the early stage, thus delaying the best treatment period. The early diagnosis of cancer has always been a difficult problem for researchers. How to combine diagnosis and treatment to treat cancer more effectively has always been the focus of scientific researchers. In terms of disease diagnosis, ultrasound, magnetic resonance imaging (MRI), CT, PET-CT and other diagnostic technologies are currently the main means of clinical application. MRI is widely used in clinical diagnosis due to its non-destructive and high-resolution without artifacts. ...

Claims

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Application Information

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IPC IPC(8): A61K49/14A61K49/18A61K41/00A61K9/51A61K47/42A61P35/00
Inventor 汪乐余彭姗胡高飞
Owner BEIJING UNIV OF CHEM TECH
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