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Allelochemical algistat and extraction and purification method thereof

A technology of allelochemical substances and purification methods, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of complex extraction process and high production cost, and achieve strong environmental affinity and low cost Inexpensive and readily available raw materials

Inactive Publication Date: 2015-07-22
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current allelochemical algae inhibitors extracted from macroalgae have the disadvantages of complicated extraction process and high production cost.

Method used

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  • Allelochemical algistat and extraction and purification method thereof
  • Allelochemical algistat and extraction and purification method thereof
  • Allelochemical algistat and extraction and purification method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The extraction steps of embodiment 1 allelochemical algastatic agent are as follows:

[0018] In the first step, the fresh seawater Cladophora collected back to the laboratory is washed, dried and crushed at 50°C;

[0019] In the second step, 500 grams of seawater Cladophora powder was dried and extracted by soaking in 3 liters of ethanol in the dark for 48 hours, and the ethanol extract was obtained through a microporous membrane with a pore size of 0.45 microns;

[0020] In the third step, the ethanol extract of seawater Cladophora is evaporated and concentrated under reduced pressure at 50° C. with a rotary evaporator to obtain an extract;

[0021] The fourth step is to dissolve the extract with distilled water, and divide the solution into two parts: soluble in water and precipitated;

[0022] In the fifth step, add 50 ml of petroleum ether to the water-soluble part, oscillate fully, and keep away from light to separate layers.

[0023] In the sixth step, take the...

Embodiment 2

[0026] The gas chromatography-mass spectrometry (GC-MS) analysis of embodiment 2 allelochemicals algae inhibitor

[0027] The chemical structure of the allelochemical algastatic agent of the present invention is analyzed by gas chromatography-mass spectrometry (GC-MS). The gas chromatography conditions are: inlet temperature: vaporization at 250°C, transfer line temperature at 280°C, flow rate: 1.50mL / min, split ratio: 100:1, column type: Hp-5MS, 30×0.25×0.25, heating program: The initial temperature is 100°C, keep 5°C / min and rise to 120°C, then heat to 280°C at a speed of 10°C / min, hold for 3min; mass spectrometry conditions are: ion source temperature: 230°C, detector voltage: 1200V , Full scan interval time: 0.5Sec, scan charge-to-mass ratio: 400-700aum, sample injection method: splitless injection, sample injection volume: 1μL. Apply the NIST mass spectrum database to analyze the mass spectrum and analyze the components. Three compounds were identified by GC-MS analysis...

Embodiment 3

[0030] The algae inhibitory effect of embodiment 3 allelochemical substance algae inhibitor to Heterocurvium akashiwo is as follows:

[0031] Different concentrations of allelochemical algae inhibitors were added to the culture medium of H. akashiwo in the logarithmic growth phase, and the number of algae cells was counted by hemocytometer to analyze the algae inhibitory effect of the allelochemicals on H. akashiwo. The results show that the allelochemical algae inhibitor of the present invention has a strong inhibitory effect on Heterocurvium akashiwo. With the increase of allelochemical algae inhibitor concentration and the prolongation of action time, the color of H. akashiwo culture solution gradually became light until it became colorless. Statistical analysis found that the 96-hour half effective concentration (EC 50 -96h) value is 5.82mg / L. The results of the algae inhibition test are shown in Table 2.

[0032] Table 2 The inhibition rate (%) of the allelochemical al...

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Abstract

The invention discloses an allelochemical algistat and an extraction and purification method thereof. The allelochemical algistat is dioctyl adipate, and can be prepared by extracting cladophora serving as large marine algae by organic solvents and then carrying out chromatographic purification by silica gel-alumina; the content of dioctyl adipate exceeds 98.6%. Compared with the prior art, the allelochemical algistat, namely dioctyl adipate, is capable of effectively inhibiting the growth of red-tide algae comprising heterosigma akashiwo and gymnodinium brevis and shows remarkable concentration-dependent effect; the EC50-96h value can be 5.82 mg / L and 2.74 mg / L respectively. The allelochemical algistat is prepared from natural plant materials and high in environmental affinity, excellent in algal inhibition effect, free of limit of region and climate, simple in extraction and purification process, and can be widely applied to control and inhibition of red-tide algae in eutrophication water in offshore areas and aquaculture ponds.

Description

technical field [0001] The invention belongs to the technical field of water body pollution control, in particular to an allelochemical algae inhibitor and an extraction and purification method thereof. Background technique [0002] Red tide control technology mainly has the following two ideas: control of nutrients such as nitrogen and phosphorus in coastal waters and control of red tide algae. At present, the technologies for controlling red tide algae include physical algae control technology, chemical algae control technology and biological algae control technology, and the commonly used methods are mainly physical and chemical methods. At present, the most widely used method of physical algae control is the method of spreading clay. The mechanism is to use the flocculation of clay particles on red tide organisms to remove red tide organisms. The most widely used method of chemical algae control is to spray chemical algaecides, mainly copper sulfate. The mechanism is to...

Claims

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Application Information

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IPC IPC(8): A01N37/04A01P13/00C07C67/48C07C69/44C02F1/50C02F103/08
Inventor 毕相东董少杰徐大为郭永军李永仁梁健
Owner TIANJIN AGRICULTURE COLLEGE
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