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A rapid staining reagent for bacterial capsule and its staining method

A rapid dyeing and bacterial capsule technology, applied in the preparation of test samples, etc., can solve the problems of unstable dyeing effect, complicated dyeing method, complicated operation process, etc., to achieve stable dyeing effect, shorten dyeing time, and prepare simple Effect

Active Publication Date: 2017-06-27
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from the existing literature reports, these methods have certain shortcomings, such as complex dyeing methods, cumbersome operations, long dyeing time, and unstable dyeing results.
For this reason, many researchers have improved the existing capsule staining method, but the improved capsule staining method still has disadvantages such as complicated operation process and long staining time.

Method used

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  • A rapid staining reagent for bacterial capsule and its staining method
  • A rapid staining reagent for bacterial capsule and its staining method
  • A rapid staining reagent for bacterial capsule and its staining method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Preparation of dye solution

[0018] 1. Accurately weigh 6.25g of basic fuchsin, put it into a mortar and grind it thoroughly, add 90ml of absolute ethanol to dissolve, then add 80ml of 4.5% carbolic acid solution and mix well.

[0019] 2. Accurately weigh 15.8g of copper sulfate and 1.98g of alum, dissolve them in 95ml of double-distilled water, and mix well.

[0020] 3. Fully mix the solutions obtained in steps 1 and 2 at a ratio of 1:2.5, filter through 2 layers of filter paper, store in a brown jar, and store at room temperature for later use.

[0021] 2. Dyeing

[0022] 1. According to the conventional method of making slides, take a clean glass slide, drop a little saline in the center of the slide, use a sterilized inoculation loop to take a little blood on the cultured Streptococcus pneumoniae lawn, Put it in the normal saline on the glass slide, spread it well, and then let it dry naturally at room temperature.

[0023] 2. Use a dropper to absorb the abov...

Embodiment 2

[0026] 1. Preparation of dye solution

[0027] 1. Accurately weigh 7.98g of basic fuchsin, put it into a mortar and grind it thoroughly, add 100ml of absolute ethanol to dissolve, then add 90ml of 4.5% carbolic acid solution and mix well.

[0028] 2. Accurately weigh 18.6g of copper sulfate and 2.05g of alum, dissolve them in 100ml of double distilled water, and mix well.

[0029] 3. Fully mix the solutions obtained in steps 1 and 2 at a ratio of 1:2.5, filter through 2 layers of filter paper, store in a brown jar, and store at room temperature for later use.

[0030] 2. Dyeing

[0031] 1. Make slides according to the conventional method, take a drop of peritoneal fluid of a dead mouse injected with Streptococcus pneumoniae, drop it on the center of a clean glass slide, spread it fully, and let it dry naturally at room temperature.

[0032] 2. Use a dropper to absorb the above dye solution, drop it on the smear area of ​​the glass slide, it is better to cover the smear area,...

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Abstract

The invention discloses a rapid staining reagent for bacterial capsule and a staining method using the reagent. The reagent is mainly prepared from basic fuchsin, copper sulfate, carbolic acid solution, alum and absolute ethanol and other reagents. The dyeing reagent has low cost and can be stored at room temperature for a long time without becoming invalid. The reagent is used to stain the bacterial capsule, and the operation is simple, requiring only one staining step. The staining method using this reagent overcomes the shortcomings of the current routine staining method of bacterial capsules; at the same time, the dyeing time of this reagent only needs 60 seconds, which greatly shortens the staining time of bacterial capsules, and the staining effect is good, and the contrast between the capsule and the background Obvious and easy to observe. It is suitable for popularization and application in the staining of bacterial capsules in teaching and scientific research.

Description

[0001] Technical field: [0002] The invention belongs to a reagent for bacterial staining, in particular to a bacterial capsule staining reagent and a dyeing method using the reagent. [0003] Background technique: [0004] Capsule is a special structure of bacteria. It refers to a layer of mucus material surrounding the cell wall of some bacteria. The main component is polysaccharide or protein polymer. Capsule is an important virulence factor for bacterial pathogenicity and an important symbol for identifying bacteria. Therefore, in teaching and scientific research, bacterial capsule staining is an important staining method. Because the bacterial capsule has a low affinity to general alkaline fuels and is not easy to be colored, it needs to be stained with a special staining method to be observed. At present, the commonly used methods are Hiss method, Muir method, Anthony staining method, ink negative staining method, carbolic acid complex red ink method, Congo red hydroch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30G01N1/31
Inventor 杨帆张晓琴赵永新李敏崔柳苏丘乐乐
Owner XINXIANG MEDICAL UNIV
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