Cystatin C detection kit and detection method of same

A technology for detection reagents and detection methods, which is applied in the biological field and can solve problems such as incompatibility of components

Inactive Publication Date: 2015-08-05
俸家富
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Problems solved by technology

Components in kits with differen...

Method used

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Embodiment Construction

[0007] 1. Sample requirements

[0008] The samples were serum or plasma collected according to standard operation (heparin anticoagulant, 0.1mg heparin anticoagulant 1.0ml blood). Samples should be stored under low temperature conditions, 2-8 ℃ airtight storage can be stable for 12 days, room temperature storage can be stable for 6 days. The sample should be equilibrated to room temperature before measurement to avoid repeated freezing and thawing of the sample. If the sample is turbid or has visible flocculent fibrin, the sample needs to be centrifuged at 3000 rpm for 3 minutes, and the supernatant is absorbed for use. Severe lipemia, hemolysis, and microbial contamination will interfere with the measurement results.

[0009] 2. Applicable instruments

[0010] Hitachi LABOSPECT (LST) series automatic biochemical analyzer.

[0011] 3. Detection method

[0012] A quality control test is required before testing the samples every day to confirm the validity of the calibratio...

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Abstract

The invention discloses a kit and a detection method of detecting latex-enhanced immune-nephelometry of cystatin C in serum or blood plasma. In the method, the cystatin C is measured in a manner of latex-enhanced immune projection nephelometry, wherein the cystatin C in a sample is subjected to a specific reaction with a latex particle-enhanced anti-cystatin C antibody to obtain an immune composition. The change of absorbancy of the immune composition under the wavelength of 546 nm is detected, wherein the change degree is in direct proportion to the content of the cystatin C in the sample, thereby obtaining the concentration of the target substance, cystatin C, in the sample. The kit of detection method of detecting latex-enhanced immune-nephelometry of cystatin C in serum or blood plasma of human body is composed of an R1 detection reagent, an R2 detection reagent and a Cys C calibration substance. The cystatin C is an ideal endogenous marker of measuring glomerular filtration rate (GFR) and is more sensitive and early-stage when compared with creatinine. The method is high in sensitivity and is high in clinical application value.

Description

technical field [0001] The invention belongs to the field of biotechnology and the field of medical immunology in vitro diagnosis, and provides a latex-enhanced immune transmission nephelometric method-LST for accurate, sensitive, rapid and quantitative detection of cystatin C (cystatin C) in human serum or plasma samples type detection method. Background technique [0002] Kidney disease is a common clinical disease, and renal dysfunction caused by various reasons is a risk factor for the progression to end-stage renal disease and cardiovascular disease. The only way to stop kidney disease from getting worse is to diagnose it early and treat it to reverse damaged kidney function. [0003] Cystatin C is a non-glycosylated protein and a kind of cysteine ​​protease inhibitor. Most nucleated cells can stably produce cystatin C. Its molecular weight is small and its production rate is stable. Due to cystatin C Protein C is not affected by changes in inflammation, gender, muscl...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/68
Inventor 俸家富李红霞刘运双张绍城杨渝伟胡冬
Owner 俸家富
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