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kefir fermented ginseng powder

A technology for fermenting ginseng and 3%-15% kefir, which is applied in the fields of food science, plant raw materials, plant/algae/fungus/moss components, etc., can solve the problem of many by-products, limited types and contents of rare ginsenosides, and difficult transformation conditions control issues

Active Publication Date: 2018-05-29
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types and contents of rare ginsenosides produced by physical transformation are limited, and steaming at high temperature for a long time will easily cause the loss of ginsenoside components
Chemical methods, including acid hydrolysis and alkali hydrolysis, such as using hydrochloric acid aqueous solution or alkaline glycerin bath to degrade ginsenosides into rare ginsenosides, the reaction conversion rate is high, but the conversion conditions are not easy to control, and there are many by-products

Method used

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  • kefir fermented ginseng powder
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  • kefir fermented ginseng powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 Kefir grain fermented ginseng powder

[0021] Ginseng was purchased in Jilin City market; kefir grains were purchased in Changchun Market

[0022] 1. Steaming: Steam the whole fresh ginseng at 80-100°C for 20-30 minutes;

[0023] 2. Beating: use a multi-function cooking machine to process for 60-90s;

[0024] 3. Sterilization: use a high-pressure steam sterilizer at 110°C to 125°C for 10 to 15 minutes;

[0025] 4. Fermentation: add 3%-15% kefir grains (mass ratio) to ginseng raw materials and cultivate at 28°C-30°C for 36-60 hours to obtain fermentation broth;

[0026] 5. Freeze-drying: the fermented liquid is freeze-dried at -55°C to -65°C for 36-48 hours to obtain fermented ginseng powder.

Embodiment 2

[0027] Ginsenoside determination of embodiment 2 Kefir grain fermented ginseng powder

[0028] 1. Preparation of Ginseng Standards

[0029] Accurately weigh 2 mg each of ginsenosides Rb1, Rg3, Rd, Rh2, RC, and Re, and add 100% methanol to 10 mL to obtain 0.2 mg / mL.

[0030] 2. Preparation of the test solution

[0031] Take 1 g of fermented ginseng powder prepared in Example 1 with a fermentation time of 48 hours and a temperature of 29°C, accurately weigh it, put it in a Soxhlet extractor, add chloroform and heat to reflux for 3 hours, discard the chloroform liquid, and evaporate the medicinal residues to dryness The solvent, together with the filter paper cylinder, was transferred into a 100mL Erlenmeyer flask, accurately added with 50mL of water-saturated n-butanol, sealed, left overnight, ultrasonically treated (power 250W, frequency 50Hz) for 30 minutes, filtered, and the filtrate was precisely weighed 25mL, placed in the evaporation Evaporate to dryness in a dish, add 1...

Embodiment 3

[0039] Example 3 Determination of basic nutritional characteristics of Kefir grain fermented ginseng powder

[0040] Ginseng dietary fiber is determined according to GB / T5009.88-2008; ginseng soluble protein is determined according to GB / T5009.5-2010, using the micro Kjeldahl method for determination; ginseng total protein is determined by Kjeldahl method; ginseng polysaccharide Phenol-sulfuric acid method was used for determination; data processing and statistics were performed by using Microsoft Excel 2007 for experimental data analysis; GeLgro software was used for TLC experimental data analysis; Graphpad Prism5 was used for drawing; Math tap was used to draw formulas.

[0041] The content changes of basic nutritional characteristics in ginseng test products are shown in Table 2 below

[0042]

[0043]From Table 2, we can see that the soluble dietary fiber, soluble protein, and total protein in the fermented ginseng test samples all increased to a certain extent.

[004...

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Abstract

The invention discloses fermented ginseng powder with Kefir grains. Fresh ginseng is steamed at 80-100°C for 20-30 minutes, beaten by a beater, 110-125°C for 10-15 minutes, and added with 3%-15% kefir grains at 28°C. ‑30°C for 36-60 hours to obtain fermentation broth; freeze-dried to obtain fermented ginseng powder; after fermentation of Kefir grains, the content of rare ginsenoside Rg3 increased by 338%, rare ginsenoside Rh2 and rare ginsenoside CK increased to a certain extent, respectively 34% and 42%, soluble dietary fiber, soluble protein, and total protein all increased to a certain extent; without adding any nutrients required by microorganisms in Kefir grains, simply using Kefir grains to ferment ginseng achieved success and exceeded expectations Results: Compared with unfermented ginseng, the fermented ginseng is softer and has a more irritating taste than fermented ginseng. It has an attractive aroma, which solves the problem that the bitter taste of ginseng products is not accepted by consumers.

Description

technical field [0001] The invention belongs to the field of medicinal food, and in particular relates to fermented ginseng powder with Kefir grains. Background technique [0002] Ginseng (Panax ginseng C.A. Mey) is a plant of the genus Panax in the Araliaceae family. Ginsenoside is the main active ingredient of ginseng, and various pharmacological activities of ginseng are related to it. There are more than 70 kinds of ginsenosides that have been isolated and identified, among which Ra, Rb, Rc, Rd, Re, Rf and Rg1 are relatively high in ginseng, accounting for more than 95% of the total ginsenosides, and are intrinsic ginsenosides in ginseng. Intrinsic ginsenosides lose their coordinating glycosyl groups after hydrolysis to obtain Rh1, CK, PPD and PPT, etc., which are rarely or almost not contained in ginseng, and are called rare ginsenosides. The less sugar groups contained in ginsenosides, the higher the bioavailability and the stronger the pharmacological activity. To i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258A23L33/00
Inventor 朴春红初琦胡耀辉于寒松刘俊梅王玉华代伟长李鹏程李想
Owner JILIN AGRICULTURAL UNIV
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