Production process of D-tryptophan

A production process and tryptophan technology are applied in the field of production technology of D-tryptophan, can solve the problems of poor quality of D-tryptophan and high purity of D-tryptophan, achieve low production cost, simple process, mild response

Inactive Publication Date: 2015-08-12
南通荣泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Purpose of the invention: the present invention provides a production process for D-tryptophan, which overcomes th

Method used

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Examples

Experimental program
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specific Embodiment 1

[0030] A production process of D-tryptophan, comprising the following steps: dissolving pharmaceutical-grade L-tryptophan, adding racemase to obtain DL-tryptophan aqueous solution, adding D-type acylase for resolution, and directly obtaining Product D-tryptophan;

[0031] The specific operation steps are as follows:

[0032] (1) Mix and dissolve pharmaceutical-grade L-tryptophan with a content of 99% and purified water to prepare an aqueous solution with a concentration of 4%;

[0033] (2) Add 200 MU of racemase to the above-prepared aqueous solution at a temperature of 35°C, and react for 6-8 hours to obtain DL-tryptophan aqueous solution;

[0034] (3) Add 200MU of D-type acylase to DL-tryptophan aqueous solution, react for 6 hours, when the measured optical rotation is 1.0, heat up to 80°C, add activated carbon for decolorization;

[0035] (4) Keep the decolorization solution warm for 30 minutes and then filter it. The clear liquid is pumped into the concentrator for conce...

specific Embodiment 2

[0036] A production process of D-tryptophan, comprising the following steps: dissolving pharmaceutical-grade L-tryptophan, adding racemase to obtain DL-tryptophan aqueous solution, adding D-type acylase for resolution, and directly obtaining Product D-tryptophan;

[0037] The specific operation steps are as follows:

[0038] (1) Mix and dissolve pharmaceutical-grade L-tryptophan with a content of 99% and purified water to prepare an aqueous solution with a concentration of 4%;

[0039] (2) Add 200 MU of racemase to the above-prepared aqueous solution at a temperature of 37°C, and react for 8 hours to obtain DL-tryptophan aqueous solution;

[0040] (3) Add 200 MU of D-type acylase to DL-tryptophan aqueous solution, react for 8 hours, when the measured optical rotation is 1.0, heat up to 90°C, add activated carbon for decolorization;

[0041] (4) Filtrate the decolorizing solution after keeping it warm for 45 minutes, and pump the clear liquid into the concentrator for concent...

specific Embodiment 3

[0042] A production process of D-tryptophan, comprising the following steps: dissolving pharmaceutical-grade L-tryptophan, adding racemase to obtain DL-tryptophan aqueous solution, adding D-type acylase for resolution, and directly obtaining Product D-tryptophan;

[0043] The specific operation steps are as follows:

[0044](1) Mix and dissolve pharmaceutical-grade L-tryptophan with a content of 99% and purified water to prepare an aqueous solution with a concentration of 4%;

[0045] (2) Add 200 MU of racemase to the above-prepared aqueous solution at a temperature of 36°C, and react for 7 hours to obtain DL-tryptophan aqueous solution;

[0046] (3) Add 200MU of D-type acylase to DL-tryptophan aqueous solution, react for 7 hours, when the measured optical rotation is 1.0, heat up to 85°C, add activated carbon for decolorization;

[0047] (4) Filtrate the decolorizing solution after keeping it warm for 40 minutes, and pump the clear liquid into the concentrator for concentra...

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Abstract

The invention discloses a production process of D-tryptophan. The production process comprises the following steps: dissolving medical-grade L-tryptophan; adding racemase to obtain a DL-tryptophan aqueous solution; and adding type-D acylase for resolution to directly obtain a product namely D-tryptophan. The production process disclosed by the invention is easily-available in raw materials, low in production cost, simple in process and mild in reaction condition, the yield reaches 90%, and the optical purity reaches 99%. The production process is mild in reaction, does not generate exhaust gas and wastewater, is not dangerous, can meet the requirements on current environmental protection and safety, and is green and safe in production, so that the D-tryptophan belongs to stage encouraged biological medicines.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a production process of D-tryptophan. Background technique [0002] As a non-protein optically active amino acid, D-tryptophan has special physiological properties and has certain value in food, feed industry and agriculture. It can be used as non-nutritive sweetener, feed additive and plant growth agent. Especially in the pharmaceutical industry, D-tryptophan is an important synthetic precursor for anticancer agents and immunosuppressants. [0003] In the prior art, the preparation methods of D-tryptophan mainly include resolution method and biological method. Among them, the resolution methods include: 1) Membrane resolution method, which can be used to separate and process a large number of racemates due to its advantages of quickness, convenience, low relative energy loss and continuous operation; 2) Enzymatic resolution There are three methods: a) Enzymatic hydrolysi...

Claims

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Application Information

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IPC IPC(8): C12P41/00C12P13/22
Inventor 曹嵘
Owner 南通荣泰生物科技有限公司
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