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Preparation method of carboxymethyl chitosan modified graphene oxide composite material

A technology of carboxymethyl chitosan and composite materials is applied in the field of preparation of graphene oxide composite materials, which can solve problems such as application limitations of chitosan, and achieve the effects of mild experimental conditions and simple operation.

Inactive Publication Date: 2015-08-19
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan is insoluble in water and can only be dissolved in some acidic aqueous solutions, which greatly limits the application of chitosan

Method used

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  • Preparation method of carboxymethyl chitosan modified graphene oxide composite material
  • Preparation method of carboxymethyl chitosan modified graphene oxide composite material
  • Preparation method of carboxymethyl chitosan modified graphene oxide composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Dissolve graphene oxide GO (50mg) in 10mL water, add activators EDC (30mg) and NHS (18mg), and stir the reaction at room temperature for 2h. During the reaction, 5 mL of an aqueous solution containing CMC (75 mg) was added dropwise, and the reaction was stirred at room temperature for 24 h. After the reaction, the solution was dialyzed with a dialysis bag (MW=100,000). After dialyzing twice with PBS, each time with a volume of 2 L, and then dialyzing with distilled water seven times, each time with a volume of 2 L, to remove impurities and by-products in the solution. The obtained product was freeze-dried to synthesize GO-CMC.

[0027] (2) Take 40 mg of GO-CMC from the above product and dissolve in an appropriate amount of distilled water, add 1.2 mg of fluorescein isothiocyanate FITC in DMSO solution, and stir at room temperature for 3 h.

[0028] (3) Add 360uL triethylamine to the above product, mix and stir for 30min, then add 240uL acetic anhydride and mix, an...

Embodiment 2

[0031] (1) Dissolve graphene oxide GO (100mg) in 25mL water, add activators EDC (45mg) and NHS (35mg) and stir at room temperature for 2h. During the reaction, 20 mL of an aqueous solution containing CMC (200 mg) was added dropwise, and the reaction was stirred at room temperature for 24 h. After the reaction, the solution was dialyzed with a dialysis bag (MW=100,000). After dialyzing twice with PBS, each time with a volume of 2 L, and then dialyzing with distilled water seven times, each time with a volume of 2 L, to remove impurities and by-products in the solution. The obtained product was freeze-dried to synthesize GO-CMC.

[0032] (2) Take 30 mg of GO-CMC from the above product and dissolve it in an appropriate amount of distilled water, add 1 mg of fluorescein isothiocyanate FITC in DMSO solution, and stir at room temperature for 3 h.

[0033] (3) Add 280uL triethylamine to the above product, mix and stir for 20min, then add 160uL acetic anhydride and mix, and react at...

Embodiment 3

[0036] (1) Dissolve graphene oxide GO (78mg) in 10mL water, add activators EDC (45mg) and NHS (40mg) and stir at room temperature for 2.5h. During the reaction, 20 mL of an aqueous solution containing CMC (120 mg) was added dropwise, and the reaction was stirred at room temperature for 24 h. After the reaction, the solution was dialyzed with a dialysis bag (MW=100,000). After dialyzing twice with PBS, each time with a volume of 2 L, and then dialyzing with distilled water seven times, each time with a volume of 2 L, to remove impurities and by-products in the solution. The obtained product was freeze-dried to synthesize GO-CMC.

[0037] (2) Take 20 mg of GO-CMC from the above product and dissolve in an appropriate amount of distilled water, add 0.8 mg of fluorescein isothiocyanate FITC in DMSO solution, and stir at room temperature for 3 h.

[0038] (3) Add 200 uL triethylamine to the above product, mix and stir for 30 min, then add 115 uL acetic anhydride, mix, and react at...

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Abstract

The invention relates to a preparation method of a carboxymethyl chitosan modified graphene oxide composite material; the preparation method includes the steps: (1) under the action of activating agents EDC and NHS, allowing graphene oxide dispersed in water to generate chemical bond connection with CMC, carrying out a reaction for 24 h at room temperature, to obtain a suspension with relatively good micro-particle dispersity, dialyzing, freeze-drying, and thus synthesizing GO-CMC; and (2) dissolving the obtained product in distilled water, adding an appropriate amount of fluorescein FITC; and acetylating the product, carrying out a stirring reaction for 24 h at room temperature, dialyzing, freeze-drying, and thus synthesizing the graphene oxide GO-CMC-FI-Ac composite material. The preparation method is simple to operate, and the product is easy to handle; and the composite material can be applicable to drug sustained release and cell imaging, and the cost is low.

Description

technical field [0001] The invention belongs to the field of preparation of graphene oxide composite materials, in particular to a method for preparing graphene oxide composite materials modified by carboxymethyl chitosan. Background technique [0002] In 2004, Geim and Novoselov of the University of Manchester in the United Kingdom successfully prepared graphene, a new two-dimensional atomic crystal composed of single atomic layers of carbon atoms connected by sp2 hybridization, for the first time using tape stripping technology. The discovery overturns scientists' predictions that ideal 2D crystalline materials cannot exist at room temperature due to thermodynamic instability. And because graphene has excellent mechanical, electrical, and optical properties, graphene-based composite materials have become a hotspot in the field of graphene applications, which have broad applications in energy storage, liquid crystal devices, electronic devices, and biomaterials. Applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/04
Inventor 朱利民潘琪霞陶磊杨卉卉吕瑶
Owner DONGHUA UNIV
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