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Magnetic carrier immobilized microorganism live bacteria preparation and preparation method and application thereof

A technology for immobilizing microorganisms and live bacteria preparations, applied in the field of environmental engineering, can solve the problems of effluent ammonia nitrogen, total phosphorus influence, unstable sludge reduction effect, etc., and achieve the effects of improving effluent water quality, facilitating digestion, and running stable.

Active Publication Date: 2015-08-19
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Judging from the published Chinese patent documents, there are very few engineering application cases of biological sludge reduction at present, because the sludge reduction effect is unstable and will have a great impact on effluent ammonia nitrogen and total phosphorus
Magnetic technology is mainly used in sewage treatment to improve COD removal rate and sludge settling performance, but has no application in in-situ sludge reduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Inoculate Acinetobacter-calcoacetcus (Acinetobacter-calcoacetcus), Candida utilis (Candida sp.), and Bacillus amyloliquefaciens (Bacillus sp.) activated on a slope into LB liquid medium respectively, and control the temperature 30°C, ventilation 1:5, culture for 24 hours, three kinds of liquid bacterial liquids were obtained;

[0048] (2) In artificially prepared sewage (glucose 0.2g / L, sodium acetate 0.3g / L, peptone 0.15g / L, ammonium sulfate 0.05g / L, urea 0.01g / L, potassium dihydrogen phosphate 0.05g / L, phosphoric acid Dipotassium hydrogen dipotassium (0.01g / L) was added with 20% activated sludge from a sewage treatment plant, and at the same time, three kinds of liquid bacteria solutions and magnetic powder were added at 5g / L respectively, aerated and stirred to cultivate, and the dissolved oxygen concentration was controlled to 2.0mg / L, and the stirring speed 150rpm, temperature control 25°C.

[0049] (3) After cultivating for 2 days to settle the sludge, empty ...

Embodiment 2

[0051] (1) Inoculate Acinetobacter-calcoacetcus (Acinetobacter-calcoacetcus), Candida utilis (Candida sp.), and Bacillus amyloliquefaciens (Bacillus sp.) activated on a slope into LB liquid medium respectively, and control the temperature 35°C, ventilation 1:5, culture for 12 hours, three liquid bacterial solutions were obtained;

[0052] (2) In artificially prepared sewage (glucose 0.2g / L, sodium acetate 0.3g / L, peptone 0.15g / L, ammonium sulfate 0.05g / L, urea 0.01g / L, potassium dihydrogen phosphate 0.05g / L, phosphoric acid Dipotassium hydrogen 0.01g / L) was added with 20% activated sludge from sewage treatment plant, and three kinds of liquid bacteria liquid and magnetic powder were added at 0.5g / L respectively, aerated and stirred for cultivation, and dissolved oxygen concentration was controlled to 3.0mg / L, and stirred Speed ​​150rpm, temperature control 35°C;

[0053] (3) After cultivating for 2 days to settle the sludge, empty the supernatant, add an equal volume of sewag...

Embodiment 3

[0055] (1) Inoculate Acinetobacter-calcoacetcus (Acinetobacter-calcoacetcus), Candida utilis (Candida sp.), and Bacillus amyloliquefaciens (Bacillus sp.) activated on a slope into LB liquid medium respectively, and control the temperature 35°C, ventilation 1:5, culture for 18 hours, three liquid bacterial solutions were obtained;

[0056] (2) In artificially prepared sewage (glucose 0.2g / L, sodium acetate 0.3g / L, peptone 0.15g / L, ammonium sulfate 0.05g / L, urea 0.01g / L, potassium dihydrogen phosphate 0.05g / L, phosphoric acid Dipotassium hydrogen 0.01g / L) was added with 25% activated sludge from sewage treatment plant, and three kinds of liquid bacteria liquid and magnetic powder were added at 3g / L respectively, aerated and agitated for cultivation, and dissolved oxygen concentration was controlled at 3.0mg / L, stirring speed 150rpm, temperature control 30℃;

[0057] (3) After cultivating for 2 days to settle the sludge, empty the supernatant, add an equal volume of sewage, add ...

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Abstract

The invention discloses a magnetic carrier immobilized microorganism live bacteria preparation and a preparation method and application thereof. The magnetic carrier immobilized microorganism live bacteria preparation comprises a magnetic carrier and biological live bacteria immobilized on the magnetic carrier. The biological live bacteria is Acinetobacter-calcoacetcus CGMCC 6820, candida utilis (Candida sp.) CGMCC 2.1495 and bacillus amyloliquefaciens (Bacillus sp.) CICC 21290. The magnetic carrier immobilized microorganism live bacteria preparation is applied to the activated sludge method or biofilm method sewage treatment process to carry out sludge in-situ decrement; the magnetic carrier immobilized microorganism live bacteria preparation is added to the sewage for treatment, so that under the condition of improving the treatment effect of a biochemical system, sludge yield of the system is reduced, and source reduction of the residual sludge is reduced. Through intensified conditioning of the live bacteria preparation, effluent COD, NH3-N, TP, TN and SS concentrations are reduced by more than 10% than original effluent, and unit COD mud production amount is reduced by more than 30%.

Description

technical field [0001] The invention relates to the technical field of environmental engineering, in particular to a magnetic carrier-immobilized live microorganism preparation for sludge in-situ reduction, a preparation method and application thereof. Background technique [0002] During the "Twelfth Five-Year Plan" period, my country's sewage treatment industry has developed rapidly, and its sewage treatment capacity has grown rapidly. In 2013, the total national wastewater discharge was 69.54 billion tons, and the sewage treatment volume was 45.61 billion tons, with a sewage treatment rate of 65%. The amount of absolute dry residual sludge produced exceeds 8 million tons. Since the sludge disposal technology in my country lags behind the sewage treatment technology, the harmlessness of the sludge produced by the sewage treatment plant is relatively low, and the development of sludge reduction and disposal technology suitable for the national conditions has broad market app...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 李明智梅荣武韦彦斐张宇王慧荣许青兰徐佳佳
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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