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Ginsenoside-containing vaccine diluent

A technology of ginsenosides and ginseng stem and leaf saponins, which is applied in the field of preparation of vaccine diluents containing ginsenosides, can solve the problems of no immune enhancement effect and weak cellular immune response, so as to improve cellular and humoral immune responses and reduce local irritation , Improving the effect of immune effect

Active Publication Date: 2015-08-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a vaccine diluent containing ginsenoside, which can overcome the shortcomings of existing vaccine diluents such as weak cellular immune response and no immune enhancement effect.

Method used

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  • Ginsenoside-containing vaccine diluent
  • Ginsenoside-containing vaccine diluent
  • Ginsenoside-containing vaccine diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Screening research of vaccine diluent formulation

[0036] 1. Materials and methods

[0037] 1. Reagents

[0038] Thimerosal sodium salt (MS), batch number: F20030210, purchased from China Pharmaceutical Group Shanghai Chemical Reagent Company; sodium chloride injection, batch number: B13020601, purchased from Zhejiang Guojing Pharmaceutical Co., Ltd.; porcine pseudorabies virus gB antibody detection kit , batch number: 09732-FC905, purchased from IDEXX company in the United States; ginseng stem and leaf saponins (GSLS), batch number: HJ140301, total saponin content 80.23%, containing Rg1 6.0%, Re 16.36%, Rbl 2.4%, Rb2 3.8%, Rf 0.1 %, Rc 3.70% and Rd 9%, purchased from Jilin Hongjiu Pharmaceutical Co., Ltd.

[0039] 2. Preparation of vaccine diluent containing ginsenosides and thimerosal

[0040] Preparation of thimerosal sodium salt solution: Weigh a certain amount of thimerosal sodium salt and fully dissolve it in physiological saline for injection to...

Embodiment 2

[0065] Example 2: Research on the synergistic adjuvant effect of ginsenosides and thimerosal

[0066] 1. Materials and methods

[0067] 1. Reagent thimerosal sodium salt (MS), batch number: F20030210, purchased from China Pharmaceutical Group Shanghai Chemical Reagent Company; sodium chloride injection, batch number: B13020601, purchased from Zhejiang Guojing Pharmaceutical Co., Ltd.; porcine pseudorabies virus gB antibody Detection kit, batch number: 09732-FC905, purchased from IDEXX company in the United States; ginsenosides (GS), total saponins greater than 90%; containing Rb1 (18.0%), Rb2 (9.5%), Rc (10.0%), Rd (7.6 %), Re (8.7%) and Rg1 (3.5%).

[0068] 2. The preparation of thimerosal sodium salt (MS) solution containing ginsenoside and thimerosal solution: take a certain amount of thimerosal sodium salt and fully dissolve it in normal saline, adjust the solution concentration to 0.02% (weight %), and store at 4°C spare.

[0069] Preparation of ginsenoside (GS) soluti...

Embodiment 3

[0088] Example 3: Vaccine diluents enhance serum antibody levels

[0089] 1. Materials and methods

[0090] 1. Reagent thimerosal sodium salt (MS), batch number: F20030210, purchased from China Pharmaceutical Group Shanghai Chemical Reagent Company; sodium chloride injection, batch number: B13020601, purchased from Zhejiang Guojing Pharmaceutical Co., Ltd.; porcine pseudorabies virus gB antibody Detection kit, batch number: 09732-FC905, purchased from IDEXX, USA; ginseng stem and leaf saponins (GSLS): batch number: HJ140301, total saponin content 80.23%, containing Rg1 6.0%, Re 16.36%, Rbl 2.4%, Rb2 3.8% , Rf 0.1%, Rc 3.70% and Rd 9%, purchased from Jilin Hongjiu Pharmaceutical Co., Ltd. HRP-labeled goat anti-mouse IgG1 and IgG2a antibodies, lot number: B13020601, were purchased from Santa Cruz Biotechnology Co., Ltd.

[0091] 2. Preparation of vaccine diluent First prepare 0.002% (weight %) thimerosal normal saline solution as solution A; then prepare 0.012% (weight %) GSLS...

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Abstract

The present invention discloses a ginsenoside-containing vaccine diluent, which comprises, by weight, 0.004-0.012% of ginseng stem leaf saponin or ginsenoside, 0.001-0.01% of thimerosal, and the balance of physiological saline. According to the present invention, after the vaccine diluted by using the vaccine diluent containing the ginseng stem leaf saponin (GSLS) or ginsenoside (GS) and the thimerosal is used to immunize animals, the immunizing effect can be substantially improved; and one of the main components of the diluent is the thimerosal having the preservative effect, such that the preservative for preventing the product from mildew is not required to be added.

Description

technical field [0001] The invention relates to a preparation method and application of a vaccine diluent containing ginsenoside. Background technique [0002] my country is the largest breeding country in the world, with about 1 billion (heads) of pigs, cattle, and sheep slaughtered each year (1). In recent years, due to the ravages of animal infectious diseases, a large number of livestock and poultry have died, which has seriously endangered the healthy development of the breeding industry. After the level of large-scale breeding is improved, the disease is more likely to spread, and the task of animal disease prevention and control is very arduous. In order to reduce the morbidity and mortality of animals, the state stipulates that certain animal infectious diseases must be immunized against vaccines. However, in recent years, the phenomenon of vaccine immunization failure in the breeding industry has occurred from time to time. After the animals are immunized and vacci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K47/24A61P37/04
Inventor 胡松华倪敬轩
Owner ZHEJIANG UNIV
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