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Preparation method for amorphous calcium phosphate nanospheres

A technology of amorphous calcium phosphate and nanospheres, applied in nanotechnology, nanotechnology, chemical instruments and methods, etc., can solve problems such as difficulty, and achieve the effect of simple preparation method, easy implementation and small size

Active Publication Date: 2015-08-26
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, all current studies use amorphous calcium phosphate solution to achieve mineralization, and it is not easy to add it to direct bonding repair materials in clinical practice.

Method used

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  • Preparation method for amorphous calcium phosphate nanospheres
  • Preparation method for amorphous calcium phosphate nanospheres
  • Preparation method for amorphous calcium phosphate nanospheres

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1. Preparation of amorphous calcium phosphate nanospheres

[0031] Dissolve a certain quality of calcium chloride and disodium hydrogen phosphate dodecahydrate in 500ml of acetic acid solution with a pH of 2.5, adjust the concentration of calcium chloride to 1mmol / L, and adjust the concentration of disodium hydrogen phosphate dodecahydrate to 0.5mmol / L to obtain a mixed solution of calcium chloride and disodium hydrogen phosphate, then add an aqueous solution of polyacrylic acid to adjust its concentration to 500 μg / ml, and mix evenly to obtain a mixed solution. The solution is sprayed into tiny droplet aerosol through an air compressed atomizer (atomization rate is 0.2mL / min), and the aerosol is blown into a spiral tube at 100°C by using nitrogen at a flow rate of 10L / min as the carrier gas. Tris-HCl buffer solution with a pH value of 8.0 and 0.5 mol / L was used as the receiving solution, and the product precipitate was obtained by filtration; the obtained r...

Embodiment 2

[0034] Embodiment 2. Preparation of amorphous calcium phosphate nanospheres

[0035] Dissolve a certain quality of calcium chloride and disodium hydrogen phosphate dodecahydrate in 500ml of acetic acid solution with a pH of 2.5, adjust the concentration of calcium chloride to 40mmol / L, and adjust the concentration of disodium hydrogen phosphate dodecahydrate to 20mmol / L L, to obtain a mixed solution of calcium chloride and disodium hydrogen phosphate, then add an aqueous solution of polyacrylic acid to adjust its concentration to 500 μg / ml, and mix evenly to obtain a mixed solution. The solution is sprayed into tiny droplet aerosol through an air compressed atomizer (atomization rate is 0.2mL / min), and the aerosol is blown into a spiral tube at 100°C by using nitrogen at a flow rate of 10L / min as the carrier gas. Tris-HCl buffer solution with a pH value of 8.0 and 0.5 mol / L was used as the receiving solution, and the product precipitate was obtained by filtration; the obtained...

Embodiment 3

[0038] Embodiment 3. Preparation of amorphous calcium phosphate nanospheres

[0039] Dissolve a certain quality of calcium chloride and disodium hydrogen phosphate dodecahydrate in 500ml of acetic acid solution with a pH of 2.5, adjust the concentration of calcium chloride to 1mmol / L, and adjust the concentration of disodium hydrogen phosphate dodecahydrate to 1mmol / L L, to obtain a mixed solution of calcium chloride and disodium hydrogen phosphate, then add an aqueous solution of polyacrylic acid to adjust its concentration to 500 μg / ml, and mix evenly to obtain a mixed solution. The solution is sprayed into tiny droplet aerosol through an air compressed atomizer (atomization rate is 0.2mL / min), and the aerosol is blown into a spiral tube at 100°C by using nitrogen at a flow rate of 10L / min as the carrier gas. Tris-HCl buffer solution with a pH value of 8.0 and 0.5 mol / L was used as the receiving solution, and the product precipitate was obtained by filtration; the obtained r...

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Abstract

The invention relates to a preparation method for amorphous calcium phosphate nanospheres. The method comprises the following steps: first, calcium salt and phosphate are dissolved in an acidic solution, then stabilizing agents are added and a mixed solution is obtained; second, the mixed solution is sprayed into minimal liquid drop aerial fog by utilization of an air compression atomizer, the aerial fog goes through a spiral pipe in a nitrogen atmosphere with a certain flow velocity and then is blown into an alkaline buffer solution, and amorphous calcium phosphate nanosphere precipitate is obtained after filtering; third, the amorphous calcium phosphate nanosphere precipitate obtained from the second step is washed and dried, and amorphous calcium phosphate nanospheres are obtained. The diameters of the obtained amorphous calcium phosphate nanospheres are less 100nm, the diameters of part nanospheres are less than 40nm, which is convenient for promotion of remineralization of dentin collagen fibers. The Ca / p ratio is adjustable, the forms of the amorphous calcium phosphate nanospheres are regular spheres, and the amorphous calcium phosphate nanospheres can exist stably for about 200h in an aqueous solution and is suitable for medicine loading and participation in bionic mineralization. The devices are simple to assemble, preparation is simple and practical, and the preparation method facilitates final achievement of large scale production.

Description

technical field [0001] The present invention relates to a preparation method of amorphous calcium phosphate nanospheres, in particular to a preparation method of amorphous calcium phosphate nanospheres whose particle size is below 40nm and is suitable for inducing the remineralization inside and outside of dentin type I collagen fibers Preparation. Background technique [0002] Amorphous calcium phosphate (ACP) is a general term for a class of calcium phosphate materials that are amorphous in X-ray diffraction. Previous studies have found that ACP is superior to other types of calcium phosphate in terms of osteoblast adhesion, osteoconductivity, biodegradation rate, and biocompatibility. These properties make it widely used in the field of biomedicine and become a research hotspot of biomineralized materials. In the process of dental caries filling, the exposed collagen fibers are destroyed by matrix metalloproteinases, which will lead to the destruction of the bonding int...

Claims

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Application Information

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IPC IPC(8): C01B25/32A61K6/033B82Y40/00B82Y30/00A61K6/838
Inventor 蔡雪韩冰李小曼朱浚鑫田福聪王晓燕
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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