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Pollen paste for plant pollination as well as preparation process and using method thereof

A pollen and paste technology, applied in the fields of botanical equipment and methods, plant cells, plant genetic improvement, etc., can solve the problems of decreased pollen vigor, influence on pollen adhesion, poor fruit development, etc., and improve vigor and germination rate. , The application is convenient and fast, and the effect of ensuring the quality of pollination

Inactive Publication Date: 2015-08-26
岑建卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of manual collection of pollen, often due to poor temperature control, long-distance transportation, long storage time, etc., or the use of frozen pollen from the previous year, the pollen viability and germination rate will decrease, and the use of powder pollen for pollination , has strict requirements on the environment, only when the temperature is suitable, the stigma of the flower can only adhere to the pollen after the mucus is secreted on the stigma
If the weather is too dry, dust, low-temperature rain, etc., will affect the adhesion of pollen on the stigma, which is the main factor for the unstable fruit setting of artificial pollination
Artificially collected pollen, in the cooling process, the temperature is slightly higher, generally more than 28 degrees Celsius will be damaged, due to reasons such as flowering or off-site use, most of them cannot be used immediately, and need to go through storage and transportation. Before storage, the pollen must be dried , if the humidity is high, it will germinate quickly at room temperature and lose its use value. At low temperature, it will suffer from high humidity. After drying, the membrane system inside the pollen will be damaged to varying degrees, and the permeability will increase. With the extension of the storage period, the pollen Internal self-generated free radicals further damage the membrane system, causing slow germination of pollen during use, low germination rate, deformed and short pollen tubes, and greatly reduced pollination quality, affecting pollination rate and pollination quality
On the fruit tree, the fruit setting rate is low, and the fruit development is not good, which affects the yield and quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] When a kind of paste pollen for plant pollination of the present invention is prepared:

[0045] first step

[0046] First boil 100 parts by weight of water, then put 30 parts by weight of polyethylene glycol into the water, then put 0.01 parts by weight of food-grade boric acid or borax into the water, and stir to dissolve the polyethylene glycol, food-grade boric acid or borax in the water , and then cool the solution to below 25 degrees Celsius;

[0047] second step

[0048]Put 0.0004 parts by weight of thiadizuron into the solution completed in the first step, stir until the thiadizuron is completely dissolved, then put 0.001 parts by weight of gibberellin and 3 parts by weight of food-grade tricalcium phosphate into the solution and stir evenly;

[0049] third step

[0050] Put 30 parts by weight of artificially collected pollen into the mixed solution completed in the second step, stir evenly to form a uniform paste, and pack it separately for use.

Embodiment 2

[0052] When a kind of paste pollen for plant pollination of the present invention is prepared:

[0053] first step

[0054] First boil 100 parts by weight of water, then put 65 parts by weight of polyethylene glycol into the water, then put 0.055 parts by weight of food-grade boric acid or borax into the water, and stir to dissolve the polyethylene glycol, food-grade boric acid or borax in the water, The solution is then cooled to below 25 degrees Celsius;

[0055] second step

[0056] Put 0.0007 parts by weight of thiadizuron into the solution completed in the first step, stir until the thiadizuron is completely dissolved, then put 0.0055 parts by weight of gibberellin and 6.5 parts by weight of food grade tricalcium phosphate into the solution and stir evenly;

[0057] third step

[0058] Put 65 parts by weight of artificially collected pollen into the mixed solution completed in the second step, stir evenly to form a uniform paste, and pack it separately for use.

Embodiment 3

[0060] When a kind of paste pollen for plant pollination of the present invention is prepared:

[0061] first step

[0062] First boil 100 parts by weight of water, then put 100 parts by weight of polyethylene glycol into the water, then put 0.1 parts by weight of food-grade boric acid or borax into the water, and stir to dissolve the polyethylene glycol, food-grade boric acid or borax in the water , and then cool the solution to below 25 degrees Celsius;

[0063] second step

[0064] Put 0.001 parts by weight of thiadizuron into the solution that was cooled to the first step, stir until the thiadidiuron is completely dissolved, then drop 0.01 parts by weight of gibberellin and 10 parts by weight of food-grade tricalcium phosphate into the solution and stir evenly;

[0065] third step

[0066] Put 100 parts by weight of artificially collected pollen into the mixed solution completed in the second step, stir evenly to form a uniform paste, and pack it separately for use.

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PUM

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Abstract

The invention belongs to the technical field of plant artificial pollination and discloses a pollen paste for plant pollination, as well as a preparation process and a using method thereof. The main technical characteristics are as follows: the pollen paste for plant pollination comprises 100 parts by weight of water, 30-100 parts by weight of polyethylene glycol, 0.01-0.1 part by weight of food grade boric acid or borax, 0.0004-0.001 part by weight of thidiazuron, 0.001-0.01 part by weight of gibberellins, 3-10 parts by weight of food grade tricalcium phosphate and 30-100 parts by weight of artificially pollinated pollen. By adopting the pollen paste for plant pollination, the polyethylene glycol is dissolved in the water to generate a strong osmotic pressure, has less possibility of freely passing through the cell wall and permeating into the living cell and is nontoxic, the cell slowly absorbs water and the damaged film system is gradually repaired by the pollen in the slow imbibition process; the activity and germination rate of the pollen are obviously higher than those of the pollen before treatment.

Description

technical field [0001] The invention belongs to the technical field of plant artificial pollination, and specifically relates to a paste pollen for plant pollination, a preparation process and a use method thereof. Background technique [0002] In agricultural and forestry production, artificial pollination is required when natural pollination is affected due to self-sterile or dioecious varieties, hybrid breeding, or bad weather during flowering. The traditional artificial pollination method is to use artificially collected pollen, mix with a certain amount of starch or fillers such as lycopodium nuts to make powder spot pollination or spray pollination. In the process of manual collection of pollen, often due to poor temperature control, long-distance transportation, long storage time, etc., or the use of frozen pollen from the previous year, the pollen viability and germination rate will decrease, and the use of powder pollen for pollination , has stricter requirements o...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01H1/02
Inventor 岑建卫
Owner 岑建卫