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Preparation method of aspergillus niger-source alpha-glucosidase solid preparation and application thereof

A technology of glucosidase and solid preparation, which is applied in the field of preparation of solid preparation of α-glucosidase derived from Aspergillus niger, can solve the problems of low enzyme activity, difficulty in realizing industrialized production, complicated extraction process, etc., and achieves low production cost and reduced production cost. The effect of production cost and production process is simple

Active Publication Date: 2015-09-02
GUANGXI NANNING ZHITIAN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the α-glucosidase strains bred by researchers at home and abroad have low enzyme activity, which makes it difficult to realize industrial production; on the other hand, the α-glucosidase is highly expressed in E. Generally, it is an intracellular enzyme, the extraction process is complicated, the recovery rate of enzyme activity is low, and it is not conducive to industrial production

Method used

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  • Preparation method of aspergillus niger-source alpha-glucosidase solid preparation and application thereof

Examples

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Effect test

Embodiment 1

[0020] Example 1: The preparation method and application of Aspergillus niger-derived α-glucosidase solid preparation 1

[0021] (1) The α-glucosidase-producing strain Aspergillus niger ( Aspergillus niger ) M1 was used as the strain, and after culturing at 32°C for 36 hours, the fermentation broth was vacuum filtered to collect mycelia.

[0022] (2) The mycelium is ground and passed through a 10-mesh sieve to make cell fragments, which are freeze-dried, measured and packaged to obtain a solid preparation of α-glucosidase.

[0023] (3) Adjust the pH to 4.5 with the maltose syrup with a solid content of 30%, add 10% (m / v) of the α-glucosidase solid preparation prepared above, and react for glycoside conversion at 55°C for 7 hours, That is, the obtained isomaltooligosaccharide syrup is determined by high-performance liquid chromatography, and the effective trisaccharide (IG+P+IG 3 ) content is 62.52%. Maltose syrup is a commercially available product, and the production com...

Embodiment 2

[0024] Embodiment 2: the preparation method and application 2 of Aspergillus niger source α-glucosidase solid preparation

[0025] (1) The α-glucosidase-producing strain Aspergillus niger ( Aspergillus niger ) M1 was used as the strain, and after culturing at 35°C for 36 hours, the fermentation broth was centrifuged to collect mycelia.

[0026] (2) Keeping the mycelium intact, after freeze-drying, measuring and dispensing, the solid preparation of α-glucosidase is obtained.

[0027] (3) Adjust the pH to 4.5 with maltose syrup with a solid content of 40%, add 15% (m / v) of the α-glucosidase solid preparation prepared above, and react for 6 hours at 55°C for glucoside conversion. That is, the obtained isomaltooligosaccharide syrup is determined by high-performance liquid chromatography, and the effective trisaccharide (IG+P+IG 3 ) content is 69.26%. Maltose syrup is a commercially available product, and the production company is Zhaoqing Huanfa Biotechnology Co., Ltd.

Embodiment 3

[0028] Embodiment 3: the preparation method and its application of Aspergillus niger source α-glucosidase solid preparation 3

[0029] (1) The α-glucosidase-producing strain Aspergillus niger ( Aspergillus niger ) M1 was used as the strain, and after culturing at 30°C for 48 hours, the fermentation broth was collected by ultrafiltration to collect mycelia.

[0030] (2) The mycelium is ground and passed through a 200-mesh sieve to make a cell powder, which is spray-dried, measured, and packaged to obtain a solid preparation of α-glucosidase.

[0031] (3) Adjust the pH to 4.5 with maltose syrup with a solid content of 10%, add 10% (m / v) of the above-prepared α-glucosidase solid preparation, and react for 8 hours at 50°C for glucoside conversion. That is, the obtained isomaltooligosaccharide syrup is determined by high-performance liquid chromatography, and the effective trisaccharide (IG+P+IG 3 ) content is 36.39%. Maltose syrup is a commercially available product, and the ...

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Abstract

The invention discloses a preparation method of an aspergillus niger-source alpha-glucosidase solid preparation and its application. The preparation method specifically relates to the following technological processes: (1) carrying out liquid culture of an aspergillus niger Mi strain, and collecting mycelium containing alpha-glucosidase by a centrifugation or membrane separation technique; (2) keeping the above mycelium intact or grinding to prepare cell debris or powder; and (3) freeze-drying or spray-drying the intact mycelium, cell debris or powder, metering and subpackaging to prepare the alpha-glucosidase solid preparation. The method provided by the invention has advantages of simple and clean production technology and low production cost. The alpha-glucosidase preparation is used for converting a starch saccharification solution or malt syrup to produce isomalto-oligosaccharide and can be used to greatly reduce production cost in comparison with a foreign commercial enzyme preparation.

Description

technical field [0001] The invention belongs to the field of microbial enzyme preparations, and in particular relates to a preparation method and application of a solid preparation of alpha-glucosidase derived from Aspergillus niger. Background technique [0002] α-glucosidase (α-glucosidase, EC 3.2.1.20) is an exoglycosidase widely present in animals, plants and microorganisms. It can recognize and hydrolyze the α-(1,4) glycosidic bond at the non-reducing end of carbohydrates, glycolipids, glycopeptides and other carbohydrate derivatives or carbohydrate analogues to release D-glucose; in addition to hydrolysis activity, a certain Some α-glucosidases also have the ability to catalyze the transglycoside reaction to varying degrees, and can transfer free glucose residues to other carbohydrates, carbohydrate derivatives or carbohydrate analog receptor substrates to form New sugars, glycolipids or glycopeptides, etc., so this type of α-glucosidase is also called glucosyltransfe...

Claims

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Application Information

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IPC IPC(8): C12N9/26C12P19/14C12R1/685
Inventor 梁智群陈桂光李玮曾伟
Owner GUANGXI NANNING ZHITIAN BIOTECH
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