NGAL rapid detection reagent and preparation method thereof

Abstract

The invention discloses an NGAL rapid detection reagent. The NGAL rapid detection reagent comprises a substrate and a cover plate covering the substrate, wherein a sample feeding region, a reaction region, a detection region and a waste liquid region are sequentially machined on the substrate; each of the sample feeding region, the reaction region, the detection region and the waste liquid region is composed of a micro-fluid channel; the sample feeding region is used for storing a polyester film for filtering a biological sample; a dry fluorescent microsphere marked antibody covers the reaction region; the dry fluorescent microsphere marked antibody comprises a fluorescent microsphere marked NGAL polyclonal antibody and a fluorescent microsphere marked goat-anti-mouse immune globulin (IgG) antibody; a detection line and a control line cover the detection region; the NGAL polyclonal antibody covers the detection line; the mouse IgG antibody covers the control line; and the waste liquid region is composed of the micro-fluid channel with a capillary action. The NGAL rapid detection reagent disclosed by the invention has the advantages of convenience for operation, rapidness in detection, high sensitivity, good accuracy and the like; and a reagent production process is simple and convenient, pollution to the environment is not caused in the production process and the cost is low.

Description

technical field [0001] The invention belongs to the field of pharmaceutical reagents, in particular to a rapid detection reagent for NGAL (neutrophil gelatinase-associated lipocalin) and a preparation method thereof. Background technique [0002] Acute kidney injury (AKI) is one of the most common emergencies in clinical departments. From mildly elevated serum creatinine to severe anuric AKI, the degree and clinical manifestations of AKI vary. AKI is closely related to the mortality of patients, the occurrence of chronic kidney disease (CKD) and the rate of maintenance dialysis. The incidence of AKI has been increasing year by year, from 610 cases (per million people) in 1988 to 2888 cases (per million people) in 2009. National statistics for the past 10 years show that the proportion of AKI patients in hospitalized patients during the same period is 0.28% to 3.19%, and as high as 30% to 50% in the ICU; the fatality rate is 14.5% to 41.9%. The incidence of AKI in criticall...

AI Technical Summary

Problems solved by technology

The operation of enzyme-linked immunoassay is easily affected by the change of enzyme activity, and the result is not very accurate; the reaction specificity of latex turbidimetry is not good, and the reagents required are relatively complicated

The immunochromatography method is easy to operate, but on the one hand, its sensitivity is not high; on the other hand, the precision of the detection results is not high due to reasons such as uneven release of markers on the binding pad, large differences in NC membranes, and uncontrollable sample chromatography speed.