Preparation method for alpha-glucosidase inhibitor

A technology for glucosidase and inhibitors, which is applied in the field of preparation of α-glucosidase inhibitors, can solve the problems of high pharmaceutical costs and achieve good inhibitory effect and high inhibitory effect

Active Publication Date: 2015-09-09
ZHEJIANG WANLI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use will cause gastrointestinal side effects such as flatulence, abdominal pain, and diarrhea, and the cost of pharmaceuticals is high

Method used

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  • Preparation method for alpha-glucosidase inhibitor
  • Preparation method for alpha-glucosidase inhibitor
  • Preparation method for alpha-glucosidase inhibitor

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Embodiment Construction

[0014] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0015] One, prepare α-glucosidase inhibitor, concrete preparation method is as follows:

[0016] Take the commercially available hijiki powder, or use existing methods to prepare it yourself. The specific method is: buy hijiki from Dongtou County, Wenzhou, Zhejiang Province, use fresh algae in May to June, and wash the surface of the sediment and salt particles with clean water , dried at 50-60°C, crushed by a large pulverizer through an 80-mesh sieve, and dried to obtain hijiki powder.

[0017] Hijiki is ultrasonically extracted with 20 times the weight of distilled water in a circulating ultrasonic instrument, the ultrasonic time is 15min, the temperature is 60°C, the solid-liquid ratio is 1:20g / ml, and the power is 1000W. Then centrifuge at 10000rpm in a centrifuge, take the supernatant, concentrate to 1 / 5 of the original volume at 60°C, a...

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Abstract

The invention relates to a preparation method for an alpha-glucosidase inhibitor. The preparation method is characterized by comprising the following steps: carrying out ultrasonic extraction on hizikia fusiforme powder in a circulating ultrasound instrument with addition of distilled water, centrifuging, taking supernatant, carrying out vacuum rotary evaporation concentration on the supernatant until the volume is one fifth of the original volume of the supernatant, adding the supernatant into absolute ethyl alcohol, leaving the solution to stand, centrifuging and freeze-drying precipitates under a vacuum condition; dissolving freeze-dried precipitates in water to prepare into a sample loading solution, injecting the sample loading solution into D311 macroporous resin for adsorption to remove protein and pigment, so as to obtain first eluent; carrying out adsorption eluting on the obtained first eluent in a DEAE-52 anion exchange fiber column and carrying out constant-gradient elution through a NaCl-Tris-HCl buffer solution system with the NaCl concentration of 0-3 M; collecting eluent under the conditions that the wavelength is 215 nm and the concentration of NaCl is 0.5-1 M, carrying out membrane concentration desalination on the eluent under a 3.5 KD tangential flow ultrafiltration membrane until the volume is one fifth of the original volume and carrying out vacuum freeze-drying so as to obtain the alpha-glucosidase inhibitor.

Description

technical field [0001] The invention relates to a preparation method of an alpha-glucosidase inhibitor. Background technique [0002] α-glucosidase is a group of hydrolytic enzymes present in the abluminal epithelial cells of the brush border membrane of the small intestine. The oligosaccharides generate glucose and other monosaccharides through the action of various enzymes on the brush border epithelial cells of the small intestine, and pass through the small intestinal mucosa Absorbed by cells to be used by the body. Studies have shown that hyperglycemia not only reduces the function of pancreatic islet B cells and insulin sensitivity, resulting in insulin resistance, but also is closely related to the chronic complications of diabetes. In the two indicators of fasting blood glucose and postprandial blood glucose, postprandial blood glucose levels increase is an important factor associated with vascular complications. Therefore, the prevention and treatment of such dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/03A61P3/10
Inventor 钱国英丁浩淼汪财生顾筱筱谢琰
Owner ZHEJIANG WANLI UNIV
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