The new application of the whole plant extract of Nepeta nepeta

A technology of plant extract, Nepeta nepeta, which is applied in the field of whole plant extract of Nepeta nepeta, can solve problems such as difficulties in diagnosis and treatment, slow local development, and metastasis of lung cancer patients

Active Publication Date: 2018-07-24
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NSCLC accounts for 80%-85% of lung cancer cases, and is the main body of lung cancer. Although the degree of malignancy is low and the local development is slow, there is a threat of metastasis in this type of lung cancer patients, which brings great difficulties to the determination of clinical diagnosis and treatment plan , so it is urgent to develop effective drugs
However, there is no research report on the anti-lung cancer activity of Nepeta chinensis extract

Method used

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  • The new application of the whole plant extract of Nepeta nepeta
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  • The new application of the whole plant extract of Nepeta nepeta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of the chloroform phase extract of the whole plant of Nepeta nepeta specifically comprises the following steps:

[0020] (1) Dry the collected branches and leaves of Nepeta nepeta in the shade to obtain 213.46g of dried whole plant of Nepeta nepeta, mix with 95% ethanol and soak overnight, then extract by ultrasonic for 3 times, the temperature of ultrasonic extraction is 50°C, the power 200W, extract 0.5h each time, then filter the ultrasonic extraction solution to obtain filtrate 1, then soak the filter residue with 95% ethanol at the same concentration as the initial ratio, and repeat the same extraction process above to obtain filtrate 2-n until the concentration is 95% % ethanol extract until colorless, finally combine all filtrates to obtain a total concentration of 95% ethanol extract.

[0021] (2) Use a rotary evaporator to carry out vacuum distillation on the total concentration of 95% ethanol extract of Nepeta nepeta to obtain the extract with ...

Embodiment 2

[0024] (1) Dry the collected branches and leaves of Nepeta nepeta in the shade to obtain 213.46g of whole plant of Nepeta nepeta dry, mix and soak with 95% ethanol overnight, then extract by ultrasonic four times, the temperature of ultrasonic extraction is 60°C, the power 200W, extract for 1 hour each time, then filter the ultrasonic extraction solution to obtain filtrate 1, then soak the filter residue with 95% ethanol at the same concentration as the initial ratio, and repeat the same extraction process above to obtain filtrate 2-n until the concentration is 95% Until the ethanol extract is colorless, finally combine all filtrates to obtain a total concentration of 95% ethanol extract.

[0025] (2) Use a rotary evaporator to carry out vacuum distillation on the total concentration of 95% ethanol extract of Nepeta nepeta to obtain the extract with a concentration of 95% ethanol, and then dissolve it in distilled water.

[0026] (3) Use petroleum ether organic solvent to extr...

Embodiment 3

[0028] (1) Dry the collected branches and leaves of Nepeta nepeta in the shade to obtain 213.46g of dried whole plant of Nepeta nepeta, mix it with 95% ethanol and soak it overnight, and then extract it five times by ultrasonic. The temperature of ultrasonic extraction is 80°C, and the power 200W, extract 1.8 h each time, then filter the ultrasonic extraction solution to obtain filtrate 1, then soak the filter residue with 95% ethanol at the same concentration as the initial ratio, repeat the same extraction process above to obtain filtrate 2-n, until the concentration is 95% % ethanol extract until colorless, finally combine all filtrates to obtain a total concentration of 95% ethanol extract.

[0029] (2) Use a rotary evaporator to carry out vacuum distillation on the total concentration of 95% ethanol extract of Nepeta nepeta to obtain the extract with a concentration of 95% ethanol, and then dissolve it in distilled water.

[0030] (3) Use petroleum ether organic solvent t...

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PUM

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Abstract

The invention discloses a novel application of a chenopodium ambrosioides whole-plant extract and belongs to the technical field of medicines and natural medicines. A chenopodium ambrosioides whole-plant chloroform extract is applied to preparation of medicines for resisting non-small cell lung cancers. A preparation method of the extract comprises the following steps: with 95% ethanol as an extracting agent, extracting the chenopodium ambrosioides whole-plant by an ultrasonic extraction method to obtain an ethanol total extract; and firstly extracting with petroleum ether and then extracting extraction raffinate with chloroform; and concentrating extract liquid to obtain a chloroform extract. The chloroform extraction part of the chenopodium ambrosioides whole-plant disclosed by the invention has obvious cell proliferation inhibition effect and cell apoptosis induction effect on the non-small cell lung cancer A549.

Description

technical field [0001] The invention relates to a new application of the whole plant extract of Nepeta nepeta, belonging to the technical field of medicine and natural medicine. Background technique [0002] Malignant tumors have always been a major disease that seriously threatens human health. Among more than 20 common cancers, lung cancer has become one of the most harmful malignant tumors. Depending on the treatment and prognosis, lung cancer can be divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC accounts for 80%-85% of lung cancer cases, and is the main body of lung cancer. Although the degree of malignancy is low and the local development is slow, there is a threat of metastasis in this type of lung cancer patients, which brings great difficulties to the determination of clinical diagnosis and treatment plan , so it is urgent to develop effective drugs. my country is rich in medicinal plant resources, which provide abundant r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P35/00
Inventor 赵扬赵婷李拓梁超潘卉冯阳
Owner KUNMING UNIV OF SCI & TECH
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