A method for preparing aglycone by fermenting soybean isoflavone glucoside from ganoderma lucidum
A fermentation technology of soybean isoflavones and Ganoderma lucidum, which is applied in the direction of fermentation, can solve the problems of β-glucosidase conversion efficiency to be improved, and achieve the effects of improving activity and absorption rate, no organic solvent residue, and rapid reproduction
Inactive Publication Date: 2019-01-04
ZHEJIANG UNIV
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At present, Aspergillus is generally considered to be an excellent strain for producing β-glucosidase, especially Aspergillus niger has the highest efficiency, but it is limited to the laboratory development stage, and the conversion efficiency of Aspergillus niger β-glucosidase still needs to be improved
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[0033] 1, the liquid fermentation culture method of Ganoderma lucidum strain
[0034] Each strain was cultured and stored in slant medium, and 4-5 pieces of 1cm 2 The mycelium block was inoculated into the seed liquid medium, and cultured at 28° C. and 180 rpm for 8 days to obtain liquid seed liquid of Ganoderma lucidum. Then inoculate various seed liquids into the fermentation medium according to the inoculum amount of 10% (v / v), and culture at 28° C. and 180 rpm for 7 days.
[0035] Slant Medium: Potato 1%, Glucose 2%, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 0.005%, agar 2%, 121 ℃ damp heat sterilization for 20min, VB 1 Filter sterilize.
[0036] Seed liquid medium: potato 1%, glucose 2%, peptone 1.8%, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 0.005%, pH 5.5, 121°C damp heat sterilization for 20min, VB 1 Filter sterilize.
[0037] Liquid fermentation medium: wort juice 4.10%, yeast extract powder 1.8%, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 0.005%, pH5.40.
[0038] Preparat...
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The invention discloses a method for preparing aglycone by transforming soybean isoflavone glycoside through lucid ganoderma fermentation. The method comprises the following steps of: inoculating lucid ganoderma into a culture medium and performing fermental cultivation to obtain fermentation liquid containing ganoderma lucidum mycelia; performing homogenization treatment on the fermentation liquid so as to crush the ganoderma lucidum mycelia and release beta-glucosaccharase into the fermentation liquid to obtain crude enzyme homogenate; and mixing the homogenate and soybean isoflavone glycoside and reacting to obtain soybean isoflavone aglycone. According to the method, a large amount of beta-glucosaccharase is obtained by deeply fermenting the lucid ganoderma to transform soybean isoflavone to prepare the aglycone, the activity of the beta-glucosaccharase is high, the product transformation ratio is high, and the activity and the absorptivity of the soybean isoflavone are greatly increased; and the transformed products are rich in bioactive substances such as Ganoderma lucidum extracellular and mycelia polysaccharides and the like, so foundation is laid for development and industrialized production of novel functional substances.
Description
technical field [0001] The invention relates to the technical field of biotransformation, in particular to a method for preparing aglycone by fermenting and transforming soybean isoflavone glycosides from Ganoderma lucidum. Background technique [0002] Ganoderma lucidum (Leyss.ex.Fr.) Karst belongs to Basidiomycetes, Hymenomycetes, Polyparales, Polyporaceae, Ganoderma, It is an edible fungus with high medicinal value and has a history of more than 2,000 years in my country. "The Pharmacopoeia of the People's Republic of China" (2000 edition) added Ganoderma lucidum, clearly pointed out that Ganoderma lucidum has the effect of "tonifying qi and calming the nerves, relieving cough and relieving asthma", and its more important pharmacological effects include anti-tumor, anti-radiotherapy and anti-chemotherapy, Anti-myocardial ischemia, regulating blood lipids, immune regulation, anti-hypoxia, scavenging free radicals and delaying aging, etc. Recent studies have also shown that...
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IPC IPC(8): C12P17/06
Inventor 何国庆崔美林袁岚杨浣漪
Owner ZHEJIANG UNIV