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Pretreatment method for enzymolysis and saccharification of bagasse

A pretreatment and bagasse technology, which is applied in the field of pretreatment of enzymatic hydrolysis and saccharified bagasse, can solve the problems of high content of fermentation inhibitors, cellulose removal, and high equipment cost requirements, and achieve high enzymolysis efficiency, low cost, and improved enzyme efficiency. The effect of saccharification rate

Active Publication Date: 2015-09-09
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the synergistic pretreatment of bagasse with NaOH and oxygen-containing reagents.
NaOH pretreatment alone needs to be carried out under high temperature and high pressure conditions, which not only requires high equipment costs, but also removes most of the cellulose, and produces a large amount of fermentation inhibitors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The pretreatment method of enzymolysis and saccharified bagasse described in this embodiment specifically includes the following steps:

[0019] (1) Crushing and sieving the bagasse to obtain a raw material with a particle size of 40 mesh, and drying at 105°C for 24 hours.

[0020] (2) Weigh 5g of bagasse and mix it with 50mL NaOH solution, add it into the autoclave and stir evenly, strictly control the oxygen pressure to 0.1MPa, and pretreat at 90°C for 0.5h. Among them: the mass percentage concentration of NaOH solution is 1%, and the mass volume ratio of bagasse and NaOH solution is 1:10 (w / v).

[0021] (3) Filter the pretreated bagasse, wash the filter bagasse with distilled water until the filtrate is neutral, and dry at 105° C. to constant weight to obtain the pretreated bagasse for later use.

[0022] The dried, pulverized and sieved untreated bagasse and pretreated bagasse samples were dried at 105°C to constant weight, and the content of cellulose, hemicellulo...

Embodiment 2

[0026] The pretreatment method of enzymolysis and saccharified bagasse described in this embodiment specifically includes the following steps:

[0027] (1) Crush and sieve the bagasse to obtain a raw material with a particle size of 60 mesh, and then dry it at 105°C for 24 hours.

[0028] (2) Weigh 10g bagasse and mix with 50mL NaOH solution, add to the autoclave and stir evenly, add H 2 o 2 , its concentration in the mixed solution is 0.3%, and it is pretreated at 70°C for 1 hour; among them: the mass percentage concentration of NaOH solution is 1.2%, and the mass volume ratio of bagasse and NaOH solution is 1:5 (w / v).

[0029] (3) Filter the pretreated bagasse, wash the filter bagasse with distilled water until the filtrate is neutral, and dry at 105° C. to constant weight to obtain the pretreated bagasse for later use.

[0030] The dried, pulverized and sieved untreated bagasse and pretreated bagasse samples were dried at 105°C to constant weight, and the content of cellu...

Embodiment 3

[0034] The pretreatment method of enzymolysis and saccharified bagasse described in this embodiment specifically includes the following steps:

[0035](1) Crushing and sieving the bagasse to obtain a raw material with a particle size of 80 meshes, and drying at 105°C for 24 hours;

[0036] (2) Weigh 5g bagasse and mix it with 100mL NaOH solution, add it into the autoclave and stir evenly, strictly control the oxygen pressure to 0.7MPa, and pretreat at 80°C for 2h. Among them: the mass percentage concentration of NaOH solution is 1.1%, and the mass volume ratio of bagasse and NaOH solution is 1:20 (w / v);

[0037] (3) Filter the pretreated bagasse, wash the filter bagasse with distilled water until the filtrate is neutral, and dry at 105° C. to constant weight to obtain the pretreated bagasse for later use.

[0038] The dried, pulverized and sieved untreated bagasse and pretreated bagasse samples were dried at 105°C to constant weight, and the content of cellulose, hemicellulos...

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Abstract

The invention discloses a pretreatment method for effective enzymolysis and saccharification of bagasse. The method comprises the following steps: drying, crushing and sieving the bagasse; and performing pretreatment on the bagasse with sodium hydroxide and an oxygen-containing reagent. The maximum removal rate of hemicellulose and lignin in the pretreated bagasse can reach 45% and 82% respectively, the cellulose enzymolysis and saccharification rate exceeds 99%, and the enzymolysis efficiency is improved by 97% in comparison with that of the untreated bagasse raw material. By adopting the method disclosed by the invention, the obstruction of hemicellulose and lignin against cellulose enzymolysis can be effectively broken, the enzymolysis resistance of bagasse is improved, and the cellulose enzymolysis and saccharification rate is sufficiently increased. By virtue of enzymolysis and saccharification, the pretreated bagasse can be applied to microbial fermentation of energy chemicals such as ethanol so as to realize effective utilization of the bagasse waste resources.

Description

technical field [0001] The invention relates to a pretreatment method for enzymolysis and saccharified bagasse, belonging to the field of biomass energy engineering. Background technique [0002] Lignocellulose is the fourth largest resource in the world. It is environmentally friendly, renewable, and has a considerable storage capacity. It is the only renewable resource that can be converted into liquid fuel. With the increasingly intensified energy crisis and environmental pollution brought by fossil resources, the conversion of lignocellulose into various forms of energy (bioethanol, biodiesel, biogas, hydrogen and fuel cells) has attracted widespread attention. [0003] Bagasse is the part obtained after mechanical pressing and extraction of sugarcane. It is the main by-product of the sugarcane sugar industry and an important lignocellulosic raw material. China is the third largest sugarcane planting country after Brazil and India, and the annual output of bagasse in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 彭林才闭帅珠陈克利
Owner KUNMING UNIV OF SCI & TECH
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