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Determination method of zolpidem content in solid tissue of poisoned rats

A detection method and zolpidem technology, which are applied in the field of detection of zolpidem content in solid tissues of poisoned rats

Active Publication Date: 2017-01-18
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no data on the exposure distribution of zolpidem in humans or other animals, especially in solid tissues (such as heart, liver, kidney, spleen, lung, brain, or muscle tissue), and it is urgent to establish the distribution data of zolpidem in vivo. Zolpidem exposure distribution data in the body (including the human body), providing a reference for the forensic detection of zolpidem

Method used

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  • Determination method of zolpidem content in solid tissue of poisoned rats
  • Determination method of zolpidem content in solid tissue of poisoned rats
  • Determination method of zolpidem content in solid tissue of poisoned rats

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Embodiment Construction

[0030] In order to clearly illustrate the solutions in the present invention, preferred embodiments are given below and detailed descriptions are given in conjunction with the accompanying drawings.

[0031] 1. Reagents

[0032] a) Solvents such as dichloromethane, ethyl acetate, ethyl methyl ketone, butyl vinyl ether, borax, sodium dihydrogen phosphate, disodium hydrogen phosphate, and sodium hydroxide are analytically pure; Zolpidem was purchased from National Narcotics laboratory; preparation of gavage solution: Weigh 600 mg of zolpidem and dissolve it in 60 mL of normal saline, mix well, and prepare a 10 mg / mL gavage solution;

[0033] b) Solvents such as methanol, acetonitrile, formic acid, and ammonium acetate are chromatographically pure;

[0034] c) Phosphate buffer solution (pH=6): Take 8.812g of disodium hydrogen phosphate (Na2HPO4·12H2O) and 27.37g of sodium dihydrogenphosphate (NaH2PO4·2H2O), add a certain amount of primary water to dissolve and dilute to 1000mL; ...

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Abstract

The invention discloses a method for detecting zolpidem content of solid tissue in an exposed rat. The method comprises the following steps of 1, sample extraction: taking a cut heart, liver, kidney, spleen, lung, brain or muscle tissue sample, and carrying out liquid-liquid extraction to extract zolpidem from the cut heart, liver, kidney, spleen, lung, brain or muscle tissue sample, and 2, instrument-based detection: carrying out qualitative and quantitative detection on the extract by a GC / NPD instrument, a GC / MS instrument or a LC-MS / MS instrument to determine zolpidem content of the cut heart, liver, kidney, spleen, lung, brain or muscle tissue sample.

Description

technical field [0001] The invention relates to a method for detecting poison in the field of criminal investigation, in particular to a method for detecting the content of zolpidem in solid tissues in poisoned rats. Background technique [0002] Zolpidem (Zolpidem): The trade name is Sinuosi, which is an imidazopyridine hypnotic. The chemical name is N,N,6-trimethyl-2-(4-tolyl)imidazo[1,2-a]-pyridine-3-acetamide, and its chemical structure is as follows: [0003] [0004] Zolpidem is generally made into tartrate, which is a colorless, odorless, yellow-white crystalline solid, slightly soluble in water, ethanol and propylene glycol, weakly alkaline, with a pKa value of 6.2. Oral absorption is rapid, has a high plasma protein binding rate (92.5%), can pass through the blood-brain barrier, and the cerebrospinal fluid concentration is 30-50% of the blood concentration, and its metabolites have no pharmacological activity, and only less than 1% of the original drug is derive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张蕾萍栾玉静杜鸿雁董颖张云峰崔冠峰何毅于忠山
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY