A kind of pineapple leaf polysaccharide and its preparation method and application

A technology of wood pineapple and polysaccharides, applied in the preparation of hypoglycemic drugs and food, the field of wood pineapple leaf polysaccharides, can solve the problems of cumbersome operation steps, unsuitable for industrial production, and high toxicity of solvents, and achieve simple process and safe solvents , low cost effect

Active Publication Date: 2017-07-11
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some literatures have reported the extraction process of polysaccharides in pineapples (Zou Yongfang, Su Yufeng, Li Qingrong, etc. Determination of polysaccharide content in water extracts of pineapples [J]. Journal of Youjiang Medical University for Nationalities, 2009, (3): 369- 370), the process uses the traditional water extraction and alcohol precipitation method to extract polysaccharides from the dry powder of pineapple fruit, and also utilizes petroleum ether to degrease in the middle, and removes protein with chloroform-n-butanol mixture (Sevage method), and the operation steps are relatively cumbersome. The solvent used is highly toxic and not suitable for industrial production

Method used

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  • A kind of pineapple leaf polysaccharide and its preparation method and application
  • A kind of pineapple leaf polysaccharide and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Take 1kg of dried wood pineapple leaves, pulverize into coarse powder, add 15L of pure water, heat at 90℃ for 2 hours, filter, add 10L of pure water to the dregs, heat at 90℃ for 1 hour, combine the extracts, concentrate the volume under reduced pressure to 0.5 L, stand at room temperature for 24 hours, filter to obtain a concentrated solution. The concentrated solution was passed through D101 macroporous adsorption resin with a column volume of 5L for saturated adsorption, and then eluted with 20L of pure water, and the eluate was concentrated to 0.5L. The above-mentioned concentrated liquid is subjected to ultrafiltration treatment with an ultrafiltration membrane with a molecular weight cut-off of 100 kDa to obtain an ultrafiltrate. Ethanol was added to the above-mentioned ultrafiltrate until the ethanol content reached 80%, and the mixture was allowed to stand at room temperature for 2 hours, filtered, and the precipitate was collected and dried in vacuum at 40° C. f...

Embodiment 2

[0026] 1Materials and methods

[0027] 1.1 Test materials

[0028] 1.1.1 Experimental animals

[0029] Forty male C57BL / KsJ-db / db diabetic mice (db / db mice), 6 weeks old, weighing 31.2±2.5 g, were purchased from Changzhou Cavens Laboratory Animal Co., Ltd.

[0030] 1.1.2 Main instruments

[0031] OneTouch blood glucose meter, Johnson & Johnson, USA

[0032] OneTouch blood glucose test strip, Johnson & Johnson, USA

[0033] Hitachi 7180 automatic biochemical analyzer, Hitachi, Japan

[0034] 1.1.3 Reagents and reagents

[0035] Metformin Hydrochloride Tablets (Shenzhen Zhonglian Pharmaceutical Co., Ltd.)

[0036] 1.2 Test method

[0037] 1.2.1 Preparation of test sample solution

[0038] Take 1kg of dried wood pineapple leaves, crush them into coarse powder, add 15L of pure water, heat at 100℃ for 1.5 hours, filter, add 10L of pure water to the dregs, heat at 100℃ for 1 hour, combine the extracts, concentrate under reduced pressure to a volume of 1L , Take 0.5L of the extract and continue to c...

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Abstract

The present invention provides a kind of wood pineapple leaf polysaccharide and its preparation methods and applications. The pineapple leaf polysaccharide is a polysaccharide with a molecular weight of less than 100kda and has the effect of lowering blood sugar. The polysaccharide is prepared by the following methods: extraction of wood pineapple leaf crude powder with hot water, after the extraction solution is concentrated, use large holes to adsorb resin to separate the column layer. Filtering the ultrafiltration fluid sinks, that is, the wood pineapple leaf polysaccharides are obtained. The preparation method of the pineapple leaf polysaccharides has the characteristics of simple craftsmanship, low cost, and suitable for industrial production. The wood pineapple leaf polysaccharides provided by the present invention can be applied to medicines and foods with blood glucose effect.

Description

Technical field [0001] The invention relates to the field of extraction and application of natural chemical components, in particular to a wood pineapple leaf polysaccharide with hypoglycemic effect, its preparation method and its application in the preparation of hypoglycemic drugs and foods. Background technique [0002] Artocarpus heterophyllus lam (Artocarpus heterophyllus lam) is a plant belonging to the genus Mooraceae. Its fruit is also called jackfruit, tree pineapple, honey melon, cow belly fruit, etc. It is a world-famous tropical fruit native to tropical Asia and is widely cultivated in tropical humid regions It has been introduced into my country for more than 1,000 years. The flesh of the wood pineapple is flat, sweet and slightly sour, non-toxic, and has the effects of producing body fluid, quenching thirst and irritability, sobering, replenishing qi, and helping digestion. Wood pineapple is a tropical tree species that integrates fruits, woody food and precious mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10A23L29/30A23L33/00
Inventor 潘小姣邓家刚
Owner GUANGXI UNIV OF CHINESE MEDICINE
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