Jackfruit leaf polysaccharide as well as preparation method and application thereof

A technology of pineapple and polysaccharide, which is applied in the preparation of hypoglycemic drugs and food, and the field of pineapple leaf polysaccharide, can solve the problems of high solvent toxicity, complicated operation steps, unsuitable for industrial production and the like, and achieves solvent safety and simple process. , the effect of low cost

Active Publication Date: 2015-09-16
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some literatures have reported the extraction process of polysaccharides in pineapples (Zou Yongfang, Su Yufeng, Li Qingrong, etc. Determination of polysaccharide content in water extracts of pineapples [J]. Journal of Youjiang Medical University for Nationalities, 2009, (3): 369- 370), the process uses the traditional water extraction and alcohol precipitation method to extract polysaccharides from the dry powder of pineapple fruit, and also utilizes petroleum ether to degrease in the middle, and removes protein with chloroform-n-butanol mixture (Sevage method), and the operation steps are relatively cumbersome. The solvent used is highly toxic and not suitable for industrial production

Method used

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  • Jackfruit leaf polysaccharide as well as preparation method and application thereof
  • Jackfruit leaf polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1kg of dry pineapple leaves, crush them into coarse powder, add 15L of pure water, heat at 90°C for 2 hours, filter, add 10L of pure water to the dregs, heat at 90°C for 1 hour, combine the extracts, concentrate under reduced pressure to a volume of 0.5 L, stood at room temperature for 24 hours, filtered to obtain a concentrated solution. The concentrated solution was saturated and adsorbed by D101 macroporous adsorption resin with a column volume of 5 L, and then eluted with 20 L of pure water, and the eluate was concentrated to 0.5 L. The above-mentioned concentrated solution is subjected to ultrafiltration treatment with an ultrafiltration membrane with a molecular weight cut-off of 100 kDa to obtain an ultrafiltrate. Add ethanol to the above-mentioned ultrafiltrate until the ethanol content reaches 80%, let it stand at room temperature for 2 hours, filter, collect the precipitate, and vacuum-dry at 40° C. for 24 hours to obtain 16.3 g of pineapple leaf polysacc...

Embodiment 2

[0026] 1 Materials and methods

[0027] 1.1 Test material

[0028] 1.1.1 Experimental animals

[0029] 40 male C57BL / KsJ-db / db diabetic mice (db / db mice), 6 weeks old, weighing 31.2±2.5g, were purchased from Changzhou Cavens Experimental Animal Co., Ltd.

[0030] 1.1.2 Main instruments

[0031] OneTouch Blood Glucose Meter, Johnson & Johnson

[0032] OneTouch Blood Glucose Test Strips, Johnson & Johnson

[0033] Hitachi 7180 automatic biochemical analyzer, Hitachi, Japan

[0034] 1.1.3 Reagents and Reagents

[0035] Metformin Hydrochloride Tablets (Shenzhen Zhonglian Pharmaceutical Co., Ltd.)

[0036] 1.2 Test method

[0037] 1.2.1 Preparation of test sample solution

[0038] Take 1kg of dried wood pineapple leaves, crush them into coarse powder, add 15L of pure water, heat at 100°C for 1.5 hours, filter, add 10L of pure water to the dregs, heat at 100°C for 1 hour, combine the extracts, and concentrate under reduced pressure to a volume of 1L , take 0.5L extract and ...

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Abstract

The invention provides a jackfruit leaf polysaccharide as well as a preparation method and an application thereof. The jackfruit leaf polysaccharide is a polysaccharide with a molecular weight of less than 100kDa and has a blood sugar-reducing function. The polysaccharide is prepared by the method comprising the following steps: extracting jackfruit leaf crude powder by hot water, concentrating the extract and then carrying out column chromatography separation by a macroporous adsorption resin, concentrating a pure water eluate and then filtering by an ultrafiltration membrane with a molecular weight cut-off of 100kDa, and carrying out alcohol precipitation on the ultrafiltrate to obtain the jackfruit leaf polysaccharide. The preparation method for the jackfruit leaf polysaccharide has the characteristics of being simple in process, low in cost, and suitable for industrial production. The jackfruit leaf polysaccharide provided by the invention can be applied to medicines and foods with a blood sugar-reducing effect.

Description

technical field [0001] The invention relates to the field of extraction and application of natural chemical components, in particular to a polysaccharide from pineapple leaves with hypoglycemic effect, its preparation method and its application in the preparation of hypoglycemic medicine and food. Background technique [0002] Artocarpus heterophyllus lam is a plant of the genus Artocarpus heterophyllus lam in the family Moraceae. Its fruit is also known as jackfruit, tree pineapple, honeydew melon, tripe fruit, etc. It is a world-renowned tropical fruit. It is native to tropical Asia and is widely cultivated in tropical humid areas. , has been introduced into my country for more than 1,000 years. The flesh of pineapple is flat, sweet and slightly sour, non-toxic, and has the effects of promoting body fluid, quenching thirst and relieving restlessness, sobering up, replenishing qi, and helping digestion. Wood pineapple is a tropical tree species integrating fruit, woody grai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10A23L1/09A23L1/29
Inventor 潘小姣邓家刚
Owner GUANGXI UNIV OF CHINESE MEDICINE
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