Preparation method for high purity fructooligosaccharides

A high-purity fructooligosaccharide technology, applied in fermentation and other fields, can solve problems such as low recycling rate, nanofiltration membrane material pollution, and increased production costs, and achieve the effects of less impurities, easy decolorization, and high purity

Inactive Publication Date: 2015-09-23
WEIDE QINGHAI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there have been reports on the use of nanofiltration membranes to refine fructo-oligosaccharides, but nano

Method used

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  • Preparation method for high purity fructooligosaccharides
  • Preparation method for high purity fructooligosaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: The effect of the amount of endoinuclease added on the yield of fructooligosaccharides.

[0029] Take 500g of fresh chicory as raw material, wash and peel it, then crush it with a blender, add 1L of pure water, stir evenly, adjust pH=5.8, add 300U pectinase, treat at 30°C for 5h, then boil 15min. Add endo-inulinase to each group of systems, the endo-inulinase derived from yeast is represented by A, and the endo-inulinase derived from Aspergillus niger is represented by B. The enzymatic activity of endo-inulinase is defined as: the amount of enzyme required to enzymolyze 1 μmol of substrate in 1 min at 30°C.

[0030] The addition of endoglycanase in each group is shown in Table 1. Treat at 30° C. for 15 hours, and then filter to remove chicory residue to obtain a fructooligosaccharide mixture. Detect the content of fructo-oligosaccharide in the fructo-oligosaccharide mixture obtained by each experimental group, and the test results are shown in Table 1. ...

Embodiment 2

[0033] Example 2: Effect of endo-inuclease action time on the yield of fructo-oligosaccharides.

[0034] Take 500g of fresh chicory as raw material, wash and peel it, then crush it with a blender, add 1L of pure water, stir evenly, adjust pH=5.8, add 200U pectinase, treat at 30°C for 5h, then boil 15min. When adding 100U and 200U of enzyme A and enzyme B to each group of systems, they were treated at 30°C for 6h, 12h, 18h, 24h, 30h, 36h, 42h, and 48h, and then filtered to remove chicory residue to obtain Fructose-oligosaccharide mixture. Detect the content of fructo-oligosaccharide in the fructo-oligosaccharide mixture obtained by each experimental group, and the test results are shown in Table 2. It can be seen from the table that the yield of fructooligosaccharide obtained by treating chicory with endo-inuclease for 18h-30h is relatively high, and the treatment time is short, which is suitable for industrial application.

[0035] The impact of table 2 endoinulinase action...

Embodiment 3

[0037] Example 3: Effect of endoinuclease action temperature on the yield of fructooligosaccharides.

[0038] Take 500g of fresh chicory as raw material, wash and peel it, then crush it with a blender, add 1L of pure water, stir evenly, adjust pH=5.8, add 300U pectinase, treat at 30°C for 5h, then boil 15min. When the addition amount of enzyme A and enzyme B in each group system was 100U and 200U respectively, they were treated at 25°C, 30°C, 35°C, 40°C, and 45°C for 24 hours, and then filtered to remove chicory residue to obtain fructooligosaccharides Mixture. Detect the content of fructo-oligosaccharide in the fructo-oligosaccharide mixture obtained by each experimental group, and the test results are shown in Table 3. As can be seen from Table 3, the yield of fructo-oligosaccharides is higher at 30-35°C.

[0039] The influence of table 3 endo-inuclease action temperature on fructo-oligosaccharide yield

[0040] temperature

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Abstract

The invention discloses a preparation method for high purity fructooligosaccharides. The method comprises the steps that 1, inulase is utilized for hydrolyzing endives to obtain a primary fructooligosaccharides solution; 2, decoloration, debitterizing and desalination treatment are conducted on the primary fructooligosaccharides solution to obtain a secondary fructooligosaccharides solution; 3, beta-D-fructosyltransferase is added into the secondary fructooligosaccharides solution, reaction is conducted for 1-8 h under the condition of 20-35 DEG C, and a third fructooligosaccharides solution is obtained; 4, a nanofiltration membrane is used for processing the third fructooligosaccharides solution obtained in the step 3, percolate is collected, and the fructooligosaccharides with the purity greater than 99% is obtained. The fructooligosaccharides is high in purity and few in impurities, the content of the fructooligosaccharides is larger than 99%, and the high purity fructooligosaccharides has important industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of fructo-oligosaccharide synthesis, and in particular relates to a preparation method of high-purity fructo-oligosaccharide. Background technique [0002] Fructose-oligosaccharides, also known as fructo-oligosaccharides, are kestose, kestose and kestrose formed by combining 1 to 3 fructosyl groups with fructosyl groups in sucrose through β(2-1) glycosidic bonds. A mixture of sugar and the like; about 60-70 grams of inulin in 100 grams of dry weight chicory, inulin is a fructan connected by a linear β-2,1-glycosidic chain, and its end is a sucrose group. Therefore, chicory powder is used as raw material to hydrolyze with endo-inulinase, and after refining, fructo-oligosaccharides can be finally obtained. [0003] Fructose-oligosaccharides have good health effects, are non-toxic, and are suitable for diabetic patients. They can prevent dental caries, regulate lipid metabolism, promote calcium absorption, low...

Claims

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Application Information

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IPC IPC(8): C12P19/18C12P19/00
Inventor 吕浩杨云
Owner WEIDE QINGHAI BIOTECH
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