Hyaluronic acid-modified liposome encapsulating whitening ingredients, its preparation method and application
A hyaluronic acid modification and whitening ingredient technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of blocked transdermal absorption, poor stability, and increased particle size, and achieve high encapsulation efficiency , simple preparation method and simple process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0045] The present invention also provides a preparation method of the above hyaluronic acid-modified liposome encapsulating whitening ingredients, comprising the following steps:
[0046] Step 1, prepare blank liposome suspension:
[0047] Weigh the following raw material components according to mass percentage, phospholipid 10-30%, cholesterol 1-10%, liposome surface positive charge control agent 1-5%, Tween-801-12%, fat-soluble antioxidant 0.1-5% %, dehydrated alcohol 50~85%, the mass sum of above each component is 100%; Phospholipid, cholesterol, liposome surface positive charge control agent, Tween-80 and fat-soluble antioxidant are dissolved in dehydrated alcohol, Obtain raw material solution, wherein, the mass ratio of the total mass of phospholipid, cholesterol, liposome surface positive charge control agent, Tween-80 and fat-soluble antioxidant to dehydrated alcohol is 1:1~1:6; Rotary evaporation, control the temperature of the water bath at 40-60°C, the rotational s...
Embodiment 1
[0065] A hyaluronic acid-modified liposome encapsulating arbutin, the specific preparation method is as follows:
[0066] Step 1, prepare blank liposome suspension
[0067] Dissolve 3.50g of soybean lecithin, 1.00g of cholesterol, 0.12g of vitamin E, 1.20g of Tween-80, and 0.30g of octadecylamine in 35mL of absolute ethanol, and dissolve in a water bath at 50°C to obtain a bright yellow solution. Pour into a round-bottom flask, and evaporate the solvent on a rotary evaporator, the temperature of the water bath is 50°C, the rotation speed is 10r / min, and the vacuum degree is maintained at 0.01MPa. After a uniform lipid film is formed on the wall of the flask, turn off the vacuum pump and remove the flask. .
[0068] Take 6.54g of trehalose and add it to 150mL of phosphate buffer solution with a pH of 6.5. Preheat the buffer solution at 60°C. Take the preheated buffer solution and add it to the flask in step 1. Shake it while adding it until it becomes a light yellow emulsion. ...
Embodiment 2
[0077] A hyaluronic acid-modified liposome encapsulating kojic acid and vitamin C, the specific preparation method is as follows:
[0078] Step 1, prepare blank liposome suspension
[0079] Dissolve 11.71g of dipalmitate phosphatidylcholine, 0.40g of cholesterol, 0.20g of butyl hydroxyanisole, 1.80g of Tween-80, and 0.38g of phosphatidylethanolamine in 35mL of absolute ethanol, and dissolve in a water bath at 50°C. Obtain a bright yellow solution, pour the solution into a round-bottomed flask, and evaporate the solvent on a rotary evaporator, the temperature of the water bath is 50°C, the rotation speed is 50r / min, and the vacuum is maintained at 0.02MPa, until a uniform lipid layer is formed on the wall of the flask Turn off the vacuum pump after the membrane and remove the flask.
[0080]Take 3.10 g of trehalose and mannitol in a mass ratio of 1:1 and add it to 150 mL of phosphate buffer solution with a pH of 6.8, preheat the buffer solution at 60°C, take the preheated buff...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size | aaaaa | aaaaa |
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More