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Method for extracting smallanthus sonchifolius plant extract, extract and application

A plant extract and extraction method technology, applied in the field of yacon plant extract, can solve the problems of limited use, unsatisfactory whitening effect, stable skin irritation formula, etc., and achieve the effect of strong practicability and broad market prospects

Inactive Publication Date: 2015-09-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the unsatisfactory whitening effect and other problems (such as skin irritation caused by adding the above-mentioned whitening actives to cosmetics, formulation stability problems, etc.), the use of these ingredients is limited.

Method used

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  • Method for extracting smallanthus sonchifolius plant extract, extract and application
  • Method for extracting smallanthus sonchifolius plant extract, extract and application
  • Method for extracting smallanthus sonchifolius plant extract, extract and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of yacon extract

[0028] Yacon leaves or tubers produced in Yunnan are used in this embodiment, but not limited thereto. In addition, the percentages mentioned in the examples all represent mass percentage concentrations unless otherwise specified.

[0029] (1) Add 1000 mL of 70% ethanol to 100 g of yacon leaves, ultrasonicate at room temperature for 1 hour, extract twice, and then filter. Concentrate the solvent of the filtrate to 50ml under reduced pressure, put it on the AB-8 resin column, and use deionized water, 30% ethanol, 70% ethanol and 95% ethanol to elute (each 500ml) successively, collect and remove the solvent under reduced pressure after collecting respectively, The dried solid was obtained and weighed to obtain 2.95 g of the 30% ethanol part of the yacon leaf, 9.89 g of the 70% ethanol part, and 2.45 g of the 95% ethanol part.

[0030] (2) Add 1000 mL of 70% ethanol to 100 g of yacon root, ultrasonicate at room temperature...

Embodiment 2

[0031] Embodiment 2: the detection of antioxidant activity

[0032]The yacon extract was prepared according to Example 1, and its free radical scavenging activity was measured by the DPPH method. The determination steps are: the yacon extract is prepared into an aqueous solution (mass volume concentration: 0.05 mg / mL) with deionized water, pipette 2 mL into a 10 mL stoppered test tube, and add 2 mL of DPPH (1,1-diphenyl -2-trinitrophenylhydrazine) ethanol solution (molar volume concentration is 2*10 -4 mol / L), mix well, let stand at room temperature, measure the absorbance T of 517nm wavelength with a spectrophotometer after 30min; measure the absorbance C after mixing 2mL DPPH solution and 2mL ethanol at the same time, the absorbance C after mixing 2mL deionized water and 2L ethanol Absorbance C 0 , and the absorbance T after mixing 2mL extract with 2mL ethanol solution 0 . Three parallel measurements were taken, the average value was taken, and the free radical scavengin...

Embodiment 3

[0037] Example 3: Detection of Elastase Inhibition

[0038] The yacon extracts prepared according to Example 1 were dissolved in deionized water to prepare an aqueous solution (mass volume concentration of 0.5 mg / ml) for the determination of elastase inhibitory activity. The assay steps are: add 10 μL sample solution and 130 μL containing 1.015 mM reaction substrate N-succinyl-propionamide-propionamide-propionamide-p-nitroaniline (English name Succ-Ala-Ala) to a 96-well plate -Ala-pNA, CAS number: 52299-14-6) in 0.1M Tris-Cl buffer solution (pH8.0), incubate at 25°C for 5 minutes, add 15μL elastase solution (0.5U / ml), continue Incubate at 25°C for 30min, then measure the absorbance A at 410nm wavelength with a microplate reader 410 . The sample aqueous solution was replaced with deionized water, and the absorbance was also measured as a reference solution, and the results are shown in Table 2.

[0039] The formula for calculating the inhibition rate of elastase activity is:...

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Abstract

The invention discloses a method for extracting a smallanthus sonchifolius plant extract. The method comprises the following steps: adding an ethanol or ethanol aqueous solution as an extracting agent into pre-processed leaves or tuberous roots of smallanthus sonchifolius, ultrasonically filtering, and concentrating a filtrate; and separating a concentrated solution through macroporous resin column chromatography, adopting gradient elution in column chromatography, eluting by using deionized water at first, then, carrying out gradient elution by using an ethanol aqueous solution having gradually increased mass percent concentrations sequentially and respectively, and post-processing to obtain the extract. The invention further discloses the smallanthus sonchifolius plant extract prepared through the method and an application method of the extract. According to the invention, leaves or tuberous roots of smallanthus sonchifolius are adopted as the raw materials; by means of a simple extraction process, extracted components having different activities and the effects of whitening skin and resisting aging can be obtained; the extracted components can be collected in a classified manner according to practical requirements; a required product can be further prepared; and the smallanthus sonchifolius plant extract is high in practicability and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting yacon plant extract, the extract and its application as whitening and anti-aging actives and the whitening and anti-aging actives. Background technique [0002] Among the determinants of human skin color, the activity of melanocytes, which produce melanin in the human body, occupies the most important position. Studies have shown that human melanocytes produce melanin through the activities of various enzymes (for example, tyrosinase), and the formation of melanin is also affected by genetic factors, hormone secretion, physiological factors related to stress and environmental factors (for example, effects of ultraviolet radiation). Melanin has the functions of blocking the sun's ultraviolet rays, protecting the skin organs under the dermis, removing free radicals produced in skin tissues, and protecting the proteins and genes in the s...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61Q19/00A61Q19/02A61Q19/08
Inventor 文岳中
Owner ZHEJIANG UNIV
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