Immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and preparation method thereof

A technology of microbial immobilization and petroleum hydrocarbons, applied in the field of environmental remediation, can solve the problems of low efficiency of in-situ bioaugmentation remediation, reduced bioavailability, and complex composition, so as to improve the quality of micro-ecological environment, reduce processing costs, and improve micro-ecology. Environmental Quality Effects

Inactive Publication Date: 2015-09-30
NANKAI UNIV
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Problems solved by technology

For example, in situ bioaugmented remediation is inefficient due to the compl...

Method used

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  • Immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and preparation method thereof
  • Immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and preparation method thereof
  • Immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and preparation method thereof

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Embodiment

[0024] Petroleum hydrocarbon-degrading bacteria Corynebacterium variabile (Corynebacterium variabile) B05 (HRJ4) (preservation number CGMCC No.10134) was immobilized, and the immobilization selected biochar and sodium alginate as immobilized carriers, and the petroleum hydrocarbon-degrading bacteria culture solution (B), The ratio of biochar (BC) and sodium alginate (SA) prepared at 700 °C was 100:5:2. First, centrifuge the cultured Corynebacterium variabile (Corynebacterium variabile) HRJ4 bacterial solution to the logarithmic phase, discard the supernatant, and rinse with sterile water twice to ensure that the bacterial solution does not contain other carbon sources, and then resuspend The bacterial suspension was obtained in an equal amount of sterile water. Mix BC and bacterial suspension at a ratio of 5:100 (w / v) for two hours to allow bacteria to adsorb on the surface of BC or enter the pores of BC, thus we obtain B-BC. Then add 2% (w / v) sodium alginate to the mixed sol...

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Abstract

The invention relates to an immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and a preparation method thereof. A salt/alkali-tolerant petroleum hydrocarbon degradation bacterium variant corynebacterium B05: (Corynebacterium variabile) HRJ4 (the collection number is CGMCC No.10134) is screened from alkaline-saline petroleum-polluted soil, and sodium alginate is added into a carrier biochar to prepare immobilized bacterium pellets which are stored and used for treating petroleum-hydrocarbon-polluted soil. The immobilized bacterium pellets are prepared by mixing the bacterium suspension, biochar and sodium alginate in a v/w/w ratio of 100:5:2. The invention provides a preparation method of the immobilized bacterium pellets. The immobilized microbial inoculant has the advantages of simple technique, obvious advantages of the degradation bacterium, and high stability, is beneficial to the petroleum hydrocarbon degradation bacterium to adapt to the new polluted environment, enhances and improves the microecological environment quality of the soil, does not cause secondary pollution to the environment, and can obviously enhance the degradation efficiency of petroleum hydrocarbons in the alkaline-saline soil.

Description

technical field [0001] The present invention relates to a microbial immobilized bacterial agent for repairing petroleum hydrocarbon polluted saline-alkali soil and a preparation method thereof, in particular to a method for immobilizing the screened high-efficiency petroleum hydrocarbon-degrading bacteria and applying it to repair saline-alkali petroleum-polluted environment The technical method belongs to the technical field of environmental restoration. Background technique [0002] During oil exploitation and oil well construction operations, it is easy to cause oil leakage and pollute the soil. Due to the specific geological conditions and unreasonable human activities, the soils of Dagang Oilfield, Daqing Oilfield, Shengli Oilfield and other oilfields in my country have different degrees of salinization. Saline-alkali soil itself has poor physical and chemical properties, and oil pollution further deteriorates its physical and chemical properties, causing significant s...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/04B09C1/10C12R1/15
Inventor 唐景春张海荣孙克静
Owner NANKAI UNIV
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