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Composite bone repairing material of two-stage three-dimensional structure and preparing method of composite bone repairing material

A three-dimensional structure, bone repair technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of fast collagen degradation, low brittleness, slow degradation of fatigue strength silk fibroin, etc., to improve mechanical properties and biocompatibility good mechanical properties, good cytocompatibility

Inactive Publication Date: 2015-10-07
SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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Problems solved by technology

[0004] In order to overcome the shortcomings of poor pore connectivity in the traditional preparation method of bone tissue engineering scaffold materials, brittleness and low fatigue strength of single hydroxyapatite in physiological environment, slow degradation rate of silk fibroin and fast degradation rate of collagen, the present invention provides a Method for preparing hydroxyapatite / silk fibroin / collagen composite scaffold material

Method used

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  • Composite bone repairing material of two-stage three-dimensional structure and preparing method of composite bone repairing material
  • Composite bone repairing material of two-stage three-dimensional structure and preparing method of composite bone repairing material
  • Composite bone repairing material of two-stage three-dimensional structure and preparing method of composite bone repairing material

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preparation example Construction

[0027] The preparation method of the composite bone repair material of the present invention is prepared by low-temperature rapid molding after blending nano-hydroxyapatite, silk fibroin and collagen. The ratio (weight ratio) of nano-hydroxyapatite, silk fibroin and collagen is 1-3:1-5:1-5. The compressive mechanical properties of the composite bone repair material are: when the maximum strain is 10%, the compressive stress is in the range of 33-47kPa, and the elastic modulus is in the range of 290-430kPa.

[0028] The osteogenic precursor cells MC3T3-E1 were cultured on the composite bone repair material, and the results showed that the composite bone repair material had good cell compatibility.

Embodiment 1

[0030] Commercially available mulberry silk, immersed in 90°C Na 2 CO 3 degumming in aqueous solution, Na 2 CO 3 The concentration of the aqueous solution is 0.5% by mass, 30 minutes each time, 3 times in total, washed with water and dried at 70°C; dissolved in CaCl at 60°C 2 , ethanol, water in a ternary solution (molar ratio 1:2:8), the dissolution time is 3h, put into a dialysis bag, and dialyze under running water flushing; Concentrate in the aqueous solution to a concentration of 10% by mass. For the preparation method of collagen gel, refer to CN1234424C (collagen-based sponge and its preparation method). Nano-hydroxyapatite, silk fibroin, and collagen gel are fully stirred and blended according to the ratio of 1:1:1 (weight ratio), and the blended gel is loaded into the syringe equipped with low-temperature rapid prototyping equipment, and the printing parameters are set ( The diameter of the printing needle is 120 μm, the printing speed is 4 mm / s, the distance bet...

Embodiment 2

[0036] Commercially available mulberry silk, immersed in 95°C Na 2 CO 3 degumming in aqueous solution, Na 2 CO 3 The concentration of the aqueous solution is 0.5%, 30 minutes each time, 3 times in total, and dried at 60° C. after washing with water. Dissolved in CaCl at 70°C 2 , ethanol, and water in a ternary solution (molar ratio 1:2:8), the dissolution time is 4 hours, put into a dialysis bag, and dialyze under running water. The dialyzed silk fibroin solution was concentrated to a concentration of 15% in polyethylene glycol (PEG) aqueous solution. For the preparation method of collagen, refer to CN1234424C (collagen-based sponge and its preparation method). Nano-hydroxyapatite, silk fibroin, and collagen gel are fully stirred and blended according to the ratio of 1:2:1 (weight ratio), and the blended gel is loaded into the syringe equipped with low-temperature rapid prototyping equipment, and the printing parameters are set ( The diameter of the printing needle is 24...

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Abstract

The invention discloses a composite bone repairing material of a two-stage three-dimensional structure and a preparing method of the composite bone repairing material. According to the composite bone repairing material, the low-temperature rapid molding technology is used for preparing three-dimensional composite gel of a large-hole structure through a mixture of nano-hydroxyapatite, fibroin and collagen according to the designed hole diameter; and a solvent is sublimated through freezing and drying at the later period to obtain micro-holes, and therefore the bone repairing support material of the two-stage three-dimensional structure is prepared. The composite material integrates the advantages of the nano-hydroxyapatite, the fibroin and the collagen; the proportion of inorganics and organics is adjusted to improve the mechanical properties of the composite material; the proportion of the fibroin and the collagen is adjusted to control the degradation time of the composite material, so that the good bone conductivity, the good mechanical property and the biological degradability are achieved, adherence and proliferation of the osteoblast are facilitated, the bone defection curing speed is greatly increased, and the bone defection curing effect is greatly improved; and the composite bone repairing material is an ideal three-dimensional printing bone repairing material.

Description

technical field [0001] The invention relates to the field of bone tissue repair materials, in particular to a hydroxyapatite / silk fibroin / collagen composite bone repair material with a two-dimensional three-dimensional structure and a preparation method thereof for low-temperature rapid prototyping. Background technique [0002] With the development of material science, life science, mechatronics and other disciplines, the research on bone repair materials has developed to the purposeful design of material components and structures, so as to achieve the organic combination with bone tissue and achieve the purpose of active repair. In addition to the macroscopic appearance structure, the construction of the internal microstructure is also very important for the construction of bone scaffold materials. At present, the manufacture of bone scaffold materials with certain bioactivity mostly utilizes the porosity of the material itself or uses a foaming agent to simulate the micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/22A61L27/12A61L27/50A61L27/56
Inventor 李瑞欣徐成李东张西正武继民李昊苏卫华王强松侍才洪赵滨李煜
Owner SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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