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Induction feed for establishment of type 2 diabetes mellitus animal model or obese animal model and application and application method of induction feed

A type 2 diabetes and animal model technology, applied in the field of biology, endocrine and metabolic diseases type 2 diabetes research, can solve problems such as high mortality, hypoinsulinemia, hyperglycemia, etc.

Inactive Publication Date: 2015-10-14
南京市溧水区人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the type 2 diabetes model has been explored for a long time, due to the unknown etiology, there are still some problems that are not easy to solve, such as postprandial insulin secretion defects and hyperinsulinemia, etc.
The toxic effect of streptozotocin and alloxan on β cells makes the experimental model extremely hyperglycemia, high mortality, and hypoinsulinemia in a short period of time, which is very different from the natural course of human type 2 diabetes, which is easy to cause Confusion of T1D Model with Type 2 Diabetes Model
The strict genetic background of spontaneous, gene knockout, transgenic and other models makes it impossible for animals of the same species with only small differences in genetic genes to replicate, which forms a huge difference with the broad susceptibility of human type 2 diabetes, and lacks the replication significance of effective models

Method used

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  • Induction feed for establishment of type 2 diabetes mellitus animal model or obese animal model and application and application method of induction feed
  • Induction feed for establishment of type 2 diabetes mellitus animal model or obese animal model and application and application method of induction feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Effects of food preservatives on blood sugar, blood lipids, body weight, and uric acid in mice.

[0023] 1.1. Experimental animals: 144 8-week-old clean Kunming mice with no special genetic background were selected, half male and half male.

[0024] 1.2. Feed production: High-fat and high-sugar feed was made by adding 10% lard and 5% sucrose to the commercially available clean-grade experimental maintenance rat food of Jiangsu Synergy Pharmaceutical Bioengineering Co., Ltd., and then added on the basis of high-fat and high-sugar feed 0.01%, 0.1%, 1.0% potassium sorbate or sodium benzoate are dried to make group feed.

[0025] 1.3. Breeding of mice: Breeding in single cage, room temperature 22-28°C, relative humidity 40-72%, light / dark time 12h / 12h per day. Rat cages were cleaned daily, free access to food and water.

[0026] 1.4. Experimental instruments: ABBOT rapid blood glucose meter; HITAICHI 7600 automatic biochemical analyzer; BIO-RAD microplate reader...

Embodiment 2

[0041] Example 2 Establishment of type 2 diabetes and obesity animal models

[0042] 2.1. Experimental animals: 48 8-week-old clean-grade Kunming mice with no special genetic background were selected, half male and half male.

[0043] 2.2. Feed production: Jiangsu Synergy Pharmaceutical Bioengineering Co., Ltd. clean-grade experimental maintenance rat food plus 10% lard and 5% sucrose to make high-fat and high-sugar feed, and then add 0.1% benzene on the basis of high-fat and high-sugar feed. Add sodium acid and dry to make mold feed.

[0044] 2.3. Breeding of mice: Breeding in single cage, room temperature 22-28°C, relative humidity 40-72%, light / dark time 12h / 12h per day. Rat cages were cleaned daily, free access to food and water.

[0045] 2.4 After 20 weeks, 32 / 48 mice's fasting blood glucose reached the diagnostic criteria for type 2 diabetes (≥7.0mmol / L). The Lee's index of 40 / 48 mice was 380±16.

[0046] The results showed that 0.1% sodium benzoate diet induced the ...

Embodiment 3

[0047] Example 3 Type 2 diabetes and obesity model establishment:

[0048] 3.1. Experimental animals: 48 8-week-old clean-grade Kunming mice with no special genetic background were selected, half male and half male.

[0049] 3.2. Feed production: Jiangsu Province Synergy Pharmaceutical Bioengineering Co., Ltd. clean-grade experimental maintenance rat food plus 10% lard and 5% sucrose to make high-fat and high-sugar feed, and then add 0.1% sorrel on the basis of high-fat and high-sugar feed Potassium acid is dried to make mold feed.

[0050] 3.3. Breeding of mice: Breeding in single cage, room temperature 22-28°C, relative humidity 40-72%, light / dark time 12h / 12h per day. Rat cages were cleaned daily, free access to food and water.

[0051] 3.4 After 25 weeks, 30 / 48 mice's fasting blood glucose reached the diagnostic criteria for type 2 diabetes (≥7.0mmol / L). The Lee's index of 35 / 48 mice was 398±22.

[0052] The results showed that 0.1% potassium sorbate diet induced the e...

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Abstract

The invention belongs to the field of biology and particularly relates to the research field of an endocrine metabolic disease type 2 diabetes mellitus, and more particularly relates to an induction feed for establishment of a type 2 diabetes mellitus animal model or an obese animal model and application and an application method of the induction feed. The invention discloses the induction feed. The diabetes mellitus induction feed comprises 0.01-0.5% of a food preservative. The invention further discloses the application and the application method for establishment of the type 2 diabetes mellitus animal model by using the diabetes mellitus induction feed. Through a selecting optimal experiment on growth indexes of a mouse by food preservatives in different contents and types, the induction feed is relatively good in inhibitory effect of intestinal flora and relatively good in blood glucose raising and weight-gaining effect. After the mouse is fed by the feed for 2-5 months, weight, blood glucose, blood lipid, urea acid, insulin and the like of the mouse are remarkably increased. A food-borne animal model which is similar in duration and pathology with type 2 diabetes mellitus with hyperinsulinemia, hyperuricemia, insulin resistance and insulin secretion deficiency is successfully established. The induction feed has remarkable innovative significance and practical value.

Description

technical field [0001] The invention belongs to the field of biology, in particular relates to the research field of endocrine and metabolic diseases type 2 diabetes, and more specifically relates to an induction feed for establishment of type 2 diabetes animal model or obesity animal model and its application and application method . Background technique [0002] Type 2 diabetes (T2D) is a multiple disease in the modern living environment. Its pathogenesis is related to environmental factors and genetic factors, and its pathogenesis is related to insulin resistance and insulin secretion defects. Research shows that diet structure or food composition is the most important environmental factor. [0003] Food preservatives are food additives used to maintain the original quality and nutritional value of food. It can inhibit the growth and reproduction of microorganisms, prevent food spoilage and prolong the shelf life. Common types of food preservatives are sodium benzoate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A01K15/02A23K1/16
CPCA01K15/02
Inventor 葛才保张力
Owner 南京市溧水区人民医院
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