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Production method of high-content galactooligosaccharide

A technology of galacto-oligosaccharide and production method, applied in the field of sugar industry, can solve the problems of restricting the entry of galacto-oligosaccharide, high price, increase cost, etc., and achieve the effects of easy large-scale promotion, improved yield, and low process requirements

Active Publication Date: 2015-10-14
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of this method requires the purchase of expensive simulated moving bed chromatographic separation equipment, which increases the cost of production and limits the entry of the galactooligosaccharide industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The preparation method of β-galactosidase is as follows:

[0033] (i) Inoculate the slanted seeds with preserved bacteria in LB solid medium, and cultivate them at 30-33° C. for 12-24 hours to obtain activated seeds;

[0034] The preparation method of LB solid medium is as follows:

[0035] Tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, agar powder 10-15h / L. Adjust the pH to 7.0 with NaOH, and steam sterilize at 15 psi for 20 minutes.

[0036] (ii) transfer the activated seeds to the LB liquid medium, shake and culture at 30-33°C for 4-8 hours, then transfer to the LB liquid medium, and shake and expand the culture at 30-33°C for 4-8 hours to obtain the seed liquid;

[0037] The preparation method of LB liquid medium is as follows:

[0038] Tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L. Adjust the pH to 7.0 with NaOH, and steam sterilize at 15 psi for 20 minutes.

[0039] (iii) transfer the seed liquid into the fermentation medium for cultiv...

Embodiment 1

[0043] A production method of high-content galacto-oligosaccharides, comprising the following steps:

[0044] (1) Dissolve lactose in water, adjust the lactose mass concentration to 45%, keep warm at 95-100°C for 20 minutes, lower the temperature to 45-50°C, then add β-galactosidase to the lactose solution, the amount of enzyme added is Add 5U of β-galactosidase to 1 gram of lactose raw material, and carry out the enzyme reaction for 10 hours at a temperature of 40°C and a pH of 5.5 to 6.0 until the mass percentage of glucose in the total sugar is 10 to 12%, and then boil to terminate the reaction to obtain a Enzyme reaction solution;

[0045] (2) Concentrate the primary enzyme reaction solution prepared in step (1) until the mass percentage of solids is 80%, add β-galactosidase, and the amount of enzyme added is 30U of β-galactosidase per gram of lactose raw material lactosidase, under the conditions of temperature 40°C and pH 5.5-6.0, carry out enzymatic reaction for 100 ho...

Embodiment 2

[0049] A production method of high-content galacto-oligosaccharides, comprising the following steps:

[0050] (1) Dissolve lactose in water, adjust the lactose mass concentration to 40%, keep warm at 95-100°C for 20 minutes, lower the temperature to 50-55°C, then add β-galactosidase to the lactose solution, the amount of enzyme added is Add 2U of β-galactosidase to the raw material of lactose, and carry out the enzyme reaction at a temperature of 55°C and a pH of 6.5 to 7.0 for 10 hours until the mass percentage of glucose in the total sugar is 5%, and then boil to terminate the reaction to obtain an enzyme reaction liquid;

[0051] (2) Concentrate the primary enzyme reaction solution prepared in step (1) until the mass percentage of solids is 55%, add β-galactosidase, and the amount of enzyme added is 100U of β-galactosidase per gram of lactose raw material Lactosidase, under the conditions of temperature 55°C and pH 6.5-7.0, carry out enzymatic reaction for 80 hours to obtain...

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Abstract

The invention relates to a production method of high-content galactooligosaccharide, which comprises the following steps: (1) adding beta-galactosidase into a lactose solution, carrying out enzyme reaction, and heating to terminate the reaction, thereby obtaining a primary enzyme reaction solution; (2) concentrating the primary enzyme reaction solution, adding beta-galactosidase, and carrying out enzyme reaction to obtain a raw sugar solution; and (3) adding activated carbon into the raw sugar solution, heating to inactivate the enzyme, carrying out ion exchange, and concentrating to obtain the galactooligosaccharide. The two enzyme reactions are utilized for the first time, so that the galactooligosaccharide content in the final product reaches 57% or above, thereby solving the problems of high galactooligosaccharide production cost and low productivity in the prior art.

Description

technical field [0001] The invention relates to a production method of high-content galacto-oligosaccharides, which belongs to the technical field of sugar industry. Background technique [0002] Galacto-oligosaccharide (GOS) is a functional oligosaccharide, which is an oligosaccharide with 1-4 galactosyl groups attached to the galactosyl side of the lactose molecule. Naturally occurring galacto-oligosaccharides are derived from animal milk. Galacto-oligosaccharides are the proliferation factors of bifidobacteria in the intestines, and the establishment of intestinal flora in infants and young children largely depends on the galacto-oligosaccharides components in breast milk. In addition, galactooligosaccharides can also improve fat metabolism and promote calcium absorption. Widely used in baked food, milk products for infants and young children, anti-caries food, etc. [0003] β-Galactosidase is an enzyme for the production of galactooligosaccharides, which can catalyze ...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 刘宗利王乃强刘峰李克文郑传宝栾庆民杨业坤张莉熊小兰
Owner BAOLINGBAO BIOLOGY
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