Novel gas-generating photoacoustic imaging contrast agent and preparation method thereof

A technology of photoacoustic imaging and photoacoustic contrast-enhanced imaging, which is applied in the field of clinical medicine, can solve problems such as expensive, unresolved, and harmful to the body, and achieve the effects of easy source, low cost, and broad application prospects

Inactive Publication Date: 2015-10-21
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to overcome the problem that the contrast agent in the prior art is harmful to the body, cannot be completely eliminated from the body, and is very expensive, and to provide a new type of gas-producing photoacoustic contrast agent that is safe, cheap, and has no side effects The preparation method thereof is used in photoacoustic imaging technology

Method used

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  • Novel gas-generating photoacoustic imaging contrast agent and preparation method thereof
  • Novel gas-generating photoacoustic imaging contrast agent and preparation method thereof
  • Novel gas-generating photoacoustic imaging contrast agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of photoacoustic imaging contrast agent

[0037] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 ).

[0038] 2. Preparation of photoacoustic imaging contrast agent: Ammonium bicarbonate lipid microspheres were prepared by thin film hydration method and extrusion method. Liposome microspheres were filled with ammonium bicarbonate saturated solution, outsourcing lipid, and then removed by dialysis Ammonium bicarbonate outside the liposomes to obtain liposome microspheres.

[0039] 3. Preparation steps:

[0040] Firstly, dissolve 10mg DPPC, 1mg DSPE-PEG, and 1mg cholesterol in 10ml chloroform. After fully dissolving to the degree of clarity and transparency, place it on a rotary evaporator and rotate it at a temperature of 45°C, for 50 minutes, and at a speed of 60r / min. Add the obtained phospholipid membrane to 5...

Embodiment 2

[0041] Example 2 Preparation of photoacoustic imaging contrast agent

[0042] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 ).

[0043] 2. Preparation of photoacoustic imaging contrast agent: Prepare ammonium bicarbonate lipid microspheres (ammonium bicarbonate saturated solution inside, lipid outsourcing) by thin film hydration method and extrusion method, and then remove the liposomes by dialysis. ammonium bicarbonate to obtain liposome microspheres.

[0044] 3. Preparation steps:

[0045] Step 1: Dissolving phospholipids: Add 1mg DSPE-PEG, 10mg DPPC and 1mg cholesterol to 10ml chloroform, shake it by hand until fully dissolved, forming a clear mixed solution of phospholipids and chloroform;

[0046] Step 2: Prepare phospholipid film: place the mixed solution obtained in step 1 on a rotary evaporator to rotate at a speed of 40r / min, time...

Embodiment 3

[0049] Example 3 Preparation of photoacoustic imaging contrast agent

[0050] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 )

[0051] 2. Preparation of photoacoustic imaging contrast agent: Prepare ammonium bicarbonate lipid microspheres (ammonium bicarbonate saturated solution inside, lipid outsourcing) by thin film hydration method and extrusion method, and then remove the liposomes by dialysis. ammonium bicarbonate to obtain liposome microspheres.

[0052] 3. Preparation steps:

[0053] Step 1: Dissolving phospholipids: Add 1mg DSPE-PEG, 10mg DPPC and 1mg cholesterol to 10ml chloroform, shake it by hand until fully dissolved, forming a clear mixed solution of phospholipids and chloroform;

[0054] Step 2: Prepare phospholipid film: place the mixed solution obtained in step 1 on a rotary evaporator to rotate at a speed of 80r / min, tim...

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Abstract

The present invention discloses a novel gas-generating photoacoustic imaging contrast agent and a preparation method thereof, and belongs to the field of clinical medicine. The novel gas-generating photoacoustic imaging contrast agent comprises a solution, microspheres and gas, wherein the solution is a saturated ammonium bicarbonate solution and is wrapped inside the microspheres, the microspheres are liposome microspheres, and the components in the formula of the wall of the liposome microspheres comprise polyethylene glycol-distearoyl phosphatidyl ethanolamine, dipalmitoyl phosphatidyl choline and cholesterol. According to the present invention, the saturated ammonium bicarbonate solution is used, such that advantages of safety, efficiency and production cost reducing are provided; the contrast agent can be used for photoacoustic imaging of superficial tissues; the produced microspheres can be stored at the low temperature; the membrane material is non-toxic and does not have side effects; the microspheres have characteristics of small particle size, stable performance, and good imaging effect; and the hemodynamics of the whole body or any one system is not affected.

Description

technical field [0001] The invention relates to a novel gas-producing photoacoustic imaging contrast agent and a preparation method thereof, belonging to the field of clinical medicine. Background technique [0002] Photoacoustic imaging is an emerging imaging technology developed in the 21st century, which is mainly used in the fields of physics and chemistry. With the continuous development of science and technology, the research of photoacoustic imaging has also made remarkable progress, and it has begun to be applied in the field of biomedicine. Photoacoustic imaging is to use the photoacoustic effect to achieve the purpose of distinguishing the different tissue structures and functions of the test object through the difference in the light absorption rate of the organism, which leads to the difference in the sound wave. It combines the high penetrating power of ultrasound and the optical The high-contrast characteristics of the system, so it has higher contrast and res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22
Inventor 夏纪筑冯罡王志刚夏晓蓉郝兰
Owner CHONGQING MEDICAL UNIVERSITY
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