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Method and special mould for preparing tissue and organ precursor with multi-branch channels

A tissue-organ, multi-branch technology, applied in the direction of prosthesis, human tubular structure device, medical science, etc., can solve the problem of single forming structure, single spindle shape of organ, no clear quantitative relationship between mold rotation speed and climbing height, etc. problems, to achieve the effect of easy operation, simple assembly and disassembly, and enlarged scope

Inactive Publication Date: 2015-11-04
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention utilizes the Weissenbery effect of non-Newtonian fluids to theoretically prepare spindle-shaped complex organ precursors with multi-branched blood vessels. However, the disadvantage of this method is that there is no clear quantitative relationship between the rotational speed of the mold and the climbing height of the liquid. It is difficult to determine the height of the inner ring mold, and the forming structure is single, and only spindle-shaped tissues and organs can be formed
In addition, a spindle body with a cone tip is formed every time, so when forming a multi-level branched vascular structure, it is difficult to position and install the inner ring mold of the second level and above on the fulcrum of the next level. In addition, the organs formed by this method The shape can only be a single spindle

Method used

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  • Method and special mould for preparing tissue and organ precursor with multi-branch channels
  • Method and special mould for preparing tissue and organ precursor with multi-branch channels
  • Method and special mould for preparing tissue and organ precursor with multi-branch channels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation of liver precursor with five-level multi-branched blood vessels and nerves

[0042] 1) A base mold with five steps and a five-level outer mold are prepared by 3D printing technology. The inner cavity of the mold is a liver-like curved surface, and the material used is silicone rubber. The five-level inner mold and the first-level inner mold are prepared respectively Contains 1 branch pipe, and the rest of the inner molds contain 3 branch pipes;

[0043] 2) Prepare 5% fibrinogen solution as matrix solution, 10IU / mL thrombin solution, purchase endothelial cells, blood cells and liver cells to make cell suspension, and the cell density is 1×10 7 cells / ml, the matrix solution and the cell suspension were mixed at a volume ratio of 4:6 to prepare a cell-containing matrix solution, and a PLGA / tetraglycol (Tetraglycol) solution with a concentration of 10% (w / v) was prepared as a synthesis solution. Polymer solution, and add 1% (w / v) heparin;

[0044] 3...

Embodiment 2

[0050] Example 2: Preparation of heart precursors with four-level multi-branch blood vessels and nerves

[0051] 1) Using 3D printing technology and mechanical processing technology to jointly prepare the base mold with four-level stepped bottom surface and four-level outer mold, the inner cavity of the mold is a heart-like curved surface, and the material used is polytetrafluoroethylene, which is prepared separately using synthetic fiber materials Four-level inner mold, the first inner mold contains 1 branch pipe, and the rest of the inner molds contain 4 branch pipes;

[0052] 2) Sodium alginate and calcium chloride were dissolved in PBS solution to make 3% sodium alginate solution as matrix solution and 2% calcium chloride solution, and endothelial cells, blood cells and cardiomyocytes were purchased to make cell suspension, The cell density of endothelial cell suspension and Xuewang cell suspension is 2×10 7 cells / ml, the cell density of the cardiomyocyte suspension was 1...

Embodiment 3

[0060] Embodiment 3: Preparing breast precursors with ten-level multi-branch blood vessels

[0061] 1) The base mold with ten steps and the outer mold with ten steps are prepared by 3D printing technology. The inner cavity of the mold is a breast-like curved surface. Contains 1 branch pipe, and other inner molds contain 1 or 2 branch pipes;

[0062] 2) Mix and dissolve 2g gelatin and 1g fibrinogen in 20mL DMEM culture medium to make gelatin / fibrinogen solution as matrix solution, 10IU / mL thrombin solution; buy endothelial cells and adipose stem cells to make cell suspension, cell density Both are 1×10 7 cells / ml; the matrix solution and the cell suspension were mixed at a volume ratio of 4:6 to make a matrix solution containing cells; a polylactic acid / isopropanol solution with a concentration of 30% (w / v) was prepared as a synthetic polymer solution, and add 30% (w / v) of sodium citrate;

[0063] 3) Part of the inner mold is vascularized to make an intravascular mold. The s...

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Abstract

The invention relates to a method and special mould for preparing a tissue and organ precursor with multi-branch channels, wherein the special mould comprises a base mould, at least one stage of inner mould and at least one stage of outer mould. The method provided by the invention comprises the steps that: the inner mould and the outer mould are arranged on the base mould in sequence according to the stages, when each stages of mould is mounted, a matrix solution containing cells is poured in the outer mould cavity, after the crosslinking of the matrix solution containing cells, the outer mould and the inner mould are removed, so that a layer of cell matrix layer with channels is formed; then the above steps are repeated, so that multiple layers of the cell matrix layer with channels are prepared in sequence, the interlayer channels are interconnected in branch-shaped, the multilayer cell matrix layer is penetrated and adhered through the synthetic polymer solution to form the tissue and organ precursor with multi-branch channels, and the shaped multi-branch channels can form the structures, such as blood vessel, nervus, bile duct, trachea and the like. The method provided by the invention overcome a defect that it is difficult to construct the complex branch channel in prior technology, and has advantages including that: the range of shaped tissue and organ is wide, the mounting of matrix materials containing high density of cells is easy to be realize.

Description

technical field [0001] The invention belongs to the technical field of artificial manufacturing of complex tissues and organs of organisms, in particular to a method for preparing a tissue and organ precursor with multi-branch channels and a special mold. Background technique [0002] There are more than ten million patients suffering from tissue defect or organ failure every year in the world. Therefore, the repair and replacement of human diseased tissues and organs is a scientific problem that needs to be solved urgently, and it is also an eye-catching scientific frontier in this century. Due to the inherent disadvantages of living tissue organ transplantation such as shortage of donors and high risk of immune rejection, it is difficult to become an effective way to solve this problem. Accordingly, Tissue Engineering (Tissue Engineering) came into being with the purpose of improving the treatment level of such diseases. Tissue Engineering is a comprehensive application of...

Claims

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Application Information

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IPC IPC(8): A61F2/02A61F2/04A61L27/22A61L27/24A61L27/20A61L27/18A61L27/54
Inventor 王小红周新伟
Owner TSINGHUA UNIV
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