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Saccharomyces cerevisiae mutant strain and application thereof

A kind of Saccharomyces cerevisiae strain and beer technology, applied in the direction of beer fermentation method, fungi, beer brewing, etc., can solve the problems of food safety and not being adopted by food enterprises

Active Publication Date: 2015-11-04
BEIJING YANJING BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used microbial improvement methods mainly include: ultraviolet mutagenesis, ion implantation mutagenesis, protoplast fusion, genetic engineering, etc., but the above-mentioned methods used in the food industry have risks of "food safety" and are generally not accepted by food. Enterprise adoption

Method used

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  • Saccharomyces cerevisiae mutant strain and application thereof
  • Saccharomyces cerevisiae mutant strain and application thereof
  • Saccharomyces cerevisiae mutant strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The preparation method of the wort culture medium: the wort (Yanjing Brewery Co., Ltd.) is sterilized to obtain the wort culture medium.

[0051] The preparation method of the wort agar medium: add 2% agar powder to the liquid wort medium to obtain the wort agar medium.

Embodiment 1

[0052] Example 1, the acquisition of Saccharomyces cerevisiae CGMCC No.10572

[0053] 1. Acquisition and identification of Saccharomyces cerevisiae CGMCC No.1784

[0054] 1. Isolation of yeast strain 8902

[0055] (1) Obtain under aseptic conditions the fermentation broth of yeast strains with excellent fermentation performance indicators in the production fermentation process.

[0056] (2) Appropriate gradient dilution of the above-mentioned fermentation broth was performed using sterile physiological saline, and each dilution was spread and separated on a wort agar medium plate, and cultured at a constant temperature of 25° C. for 3 days.

[0057] (3) Select a single colony with good separation effect and excellent colony characteristics on the above-mentioned medium plate, streak and separate it on the wort agar medium plate, culture at a constant temperature of 25°C for 3 days, repeat the operation 2-3 times, and obtain yeast strain 8902 .

[0058] 2. Morphological iden...

Embodiment 2

[0109] Example 2, Functional Research of Saccharomyces cerevisiae Mutant Strain CGMCC No.10572

[0110] 1. Fermentation

[0111] The Saccharomyces cerevisiae mutant strain CGMCC No.10572 obtained in step 2 of Example 1 was prepared at an initial concentration of 18×10 6 Each / mL was inoculated in an Erlenmeyer flask filled with 350mL wort medium (14°P), and a fermentation plug sealed with sulfuric acid liquid was installed, and fermented at a constant temperature of 10°C (initial yeast inoculum was controlled at 15×10 6 cells / mL), the fermentation broth of Saccharomyces cerevisiae mutant strain CGMCC No.10572 was collected in 10 days. During fermentation, shake the flask daily to remove CO 2 and weigh the CO 2 Lose weight, wait for CO 2 When the weight loss is less than 0.2g, it means that the fermentation is over. Using Saccharomyces cerevisiae CGMCC No.1784 as a control, the fermentation broth of Saccharomyces cerevisiae CGMCC No.1784 was obtained.

[0112] 2. Detection...

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PUM

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Abstract

The invention discloses a saccharomyces cerevisiae mutant strain and application thereof. The preservation number of the saccharomyces cerevisiae mutant strain is CGMCC 10572. Experiments show that the saccharomyces cerevisiae CGMCC NO.10572 can evidently enhance the reducing capacity of diacetyl, increase volatile ester content, lower higher alcohol content and increase beer flavor quality. The saccharomyces cerevisiae mutant strain has the advantages that a new method for applying space mutation to saccharomyces cerevisiae breeding is explored, and a precedent for applying space mutation breeding to beer production is set.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mutagenic strain of Saccharomyces cerevisiae and an application thereof. Background technique [0002] Space breeding, also known as space mutation breeding, is to use high-altitude balloons, returnable satellites, spacecraft and other spacecraft to carry crop seeds, tissues, organs or living individuals into space, and use the special environment of space to mutate biological genes and The process of selecting excellent strains by obtaining stable physiological and genetic traits through ground selection. [0003] In the field of beer production, how to improve the quality of beer, shorten the fermentation cycle, reduce production costs and reduce environmental pollution are important scientific research goals for beer production enterprises. Breeding and improving beer brewing yeast is also one of the channels to achieve the above goals. At present, the commonly use...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12C11/00C12R1/865
Inventor 郭立芸穆英健林智平贾凤超梁云朱佳静张文婧吴媛媛
Owner BEIJING YANJING BREWERY
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