Saccharomyces cerevisiae mutant strain and application thereof
A kind of Saccharomyces cerevisiae strain and beer technology, applied in the direction of beer fermentation method, fungi, beer brewing, etc., can solve the problems of food safety and not being adopted by food enterprises
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0050] Preparation method of wort culture medium: Sterilize wort (Yanjing Beer Co., Ltd.) to obtain wort culture medium.
[0051] The preparation method of the wort agar medium: adding 2% agar powder to the liquid wort medium to obtain the wort agar medium.
Example Embodiment
[0052] Example 1. Obtaining of Saccharomyces cerevisiae CGMCC No.10572
[0053] 1. Acquisition and identification of Saccharomyces cerevisiae CGMCC No.1784
[0054] 1. Isolation of yeast strain 8902
[0055] (1) Obtain a yeast strain fermentation broth with excellent fermentation performance indicators in the production and fermentation process under aseptic conditions.
[0056] (2) Dilute the above-mentioned fermentation broth in appropriate gradients with sterile physiological saline, and coat and separate each dilution on a wort agar medium plate, and cultivate at a constant temperature of 25° C. for 3 days.
[0057] (3) Select a single colony with good separation effect and excellent colony characteristics on the above-mentioned medium plate, streak separation on the wort agar medium plate, cultivate at a constant temperature of 25 ° C for 3 days, repeat the operation 2-3 times, and obtain yeast strain 8902 .
[0058] 2. Morphological identification of yeast strain 8902...
Example Embodiment
[0109] Example 2. Functional study of Saccharomyces cerevisiae mutant strain CGMCC No. 10572
[0110] 1. Fermentation
[0111] The Saccharomyces cerevisiae mutant strain CGMCC No. 10572 obtained in step 2 of Example 1 was prepared at an initial concentration of 18×10 6 inoculated into a conical flask containing 350 mL of wort medium (14°P), and installed a fermentation plug sealed with sulfuric acid, and fermented at a constant temperature of 10°C (the initial yeast inoculum was controlled at 15×10 6 1 / mL), the fermentation broth of Saccharomyces cerevisiae mutant CGMCC No. 10572 was collected in 10 days. During fermentation, shake flasks to remove CO every day 2 and weigh the CO 2 Weight loss, waiting for CO 2 The fermentation is complete when the weight loss is less than 0.2g. Using Saccharomyces cerevisiae CGMCC No. 1784 as a control, the fermentation broth of Saccharomyces cerevisiae CGMCC No. 1784 was obtained.
[0112] 2. Detection of fermentation products
[0113...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap