A Saccharomyces cerevisiae engineered strain expressing pectin esterase and its application

A technology of pectin esterase and Saccharomyces cerevisiae, which is applied in the field of fermentation engineering, can solve the problems of high viscosity of mash, blockage of conveying pipelines, and influence on ethanol production, and achieve the effects of reducing equipment burden, saving energy consumption, and reducing viscosity
CN105018364BActive Publication Date: 2019-01-29JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-01-29

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Abstract

The invention discloses a saccharomyces cerevisiae engineered strain expressing pectin esterase and application of the strain and belongs to the field of fermentation engineering. The saccharomyces cerevisiae engineered strain is characterized in that the encoding genes of the pectin esterase are fused with the encoding genes of alpha-lectin and then converted into saccharomyces cerevisiae, recombinant plasmids are integrated to the chromosome NDA of hosts to obtain the strain expressing the pectin esterase on the surfaces of cells in an anchored manner, and pectin esterase activity can reach 2.6U / g. When the strain is used for the alcohol fermentation production of raw materials such as sweet potatoes and cassavas, alcohol fermentation efficiency can be increased while fermentation liquor viscosity during fermentation can be evidently lowered, enzymatic action and saccharomyces cerevisiae metabolism are benefited, equipment burden can be lowered, and energy consumption can be reduced.
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Description

technical field

[0001] The invention relates to a strain of Saccharomyces cerevisiae strain expressing pectin esterase and application thereof, in particular to a strain of Saccharomyces cerevisiae strain expressing pectin esterase anchored on the cell surface and application thereof, belonging to the field of fermentation engineering. Background technique

[0002] The depletion of fossil fuels and the global environmental protection requirements have brought good development opportunities for the fuel alcohol industry. Fuel alcohol is a renewable and clean energy source. Adding a certain proportion of alcohol to gasoline can significantly reduce the environmental pollution caused by vehicle exhaust. my country is the world's third largest producer of fuel ethanol after the United States and Brazil, with an annual output of 1.518 million tons and increasing year by year. Starch raw material is the main raw material for the production of fuel alcohol. There are many viscous ...

Claims

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