DNA quality-based method for identification of fat and protein content in milk
A protein content, fat content technology, applied in the field of food science, to achieve the effect of broad application prospects, simple operation, and low cost
Active Publication Date: 2015-11-04
陕西华州营养健康食品技术创新中心有限公司
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Problems solved by technology
Wang Lijie et al. used near-infrared diffuse reflectance spectroscopy and partial least squares regression method to detect and analyze the protein content in milk. This method has the advantages of rapidity, low cost and non-destructive measurement of multiple components at the same time, but it requires a large amount of milk. Build a model and need a value for milk protein content in milk
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Embodiment 1
[0033] 1. Source of experimental samples
[0034]The milk samples were collected from 5 adult dairy cows in the homes of dairy farmers in the suburbs of Xi'an City, Shaanxi Province. The volume of each sample was 15 mL and put into a 15 mL centrifuge tube. After collection, immediately put them back in an ice box and store them in the refrigerator for 15 days until use.
[0035] 2. Test reagent
[0036] Ethanol (above 95% volume fraction), anhydrous sodium carbonate, sodium chloride, isoamyl alcohol and other reagents are analytical reagents or solutions, 95% ethanol, sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, Triton- x 100, Tris-Cl, EDTA-Na, sodium lauryl sulfate, concentrated hydrochloric acid, protease, EDTA, Na 2 -EDTA and glacial acetic acid were purchased from Xi'an Symbo Biological Co., Ltd.
[0037] Phosphate buffer solution (PBS) configuration: weigh NaCl 8g, KCl 0.2g, NaCl 2 HPO 4 1.44g, KH 2 PO 4 0.24g, dissolve various substan...
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The present invention discloses a DNA quality-based method for identification of fat and protein content in milk. The method uses bovine genomic DNA quality in milk to identify the content of fat and protein in milk, and establishes a linear regression equation of the content of protein in milk and bovine genomic DNA quality in milk within a certain range, or establishes a linear regression equation of the content of fat in milk and bovine genomic DNA quality in milk within a certain range, so as to determine the content of fat or protein in milk. The method for the first time establishes the relevance of bovine genomic DNA quality in milk with protein and fat content in milk, and provides a new method for the identification of nutriment quality of protein and fat in milk. The present invention has the advantages of simple operation, low cost and wide application prospects, and has great significance to nutrition quality test of milk.
Description
technical field [0001] The invention belongs to the field of food science, and specifically, mainly relies on the quality of bovine genomic DNA in milk to identify changes in fat and protein content in raw milk. This method intends to establish the correlation between the DNA content and quality of milk and the main nutritional qualities (milk fat, milk protein) of milk under refrigeration (1-5 ℃), which is beneficial to provide high-quality raw milk for dairy processing enterprises and consumers. Background technique [0002] The protein and fat content of raw milk are the two most important indicators for evaluating the nutritional quality of milk, and are also often used as the standard for raw milk purchases. The detection methods of the two indicators mostly use physical and chemical methods. [0003] There are four classical methods commonly used for protein content determination: Kjeldahl method, biuret method, Folin-phenol reagent method (Lowry method), ultraviolet ...
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IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2545/101
Inventor 刘永峰库婷高俊岭
Owner 陕西华州营养健康食品技术创新中心有限公司