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A method for extracting burdock polysaccharide from burdock root

A technology of burdock root and burdock fructooligosaccharides, which is applied in the field of extracting burdock fructooligosaccharides, can solve the problems of high loss rate of polysaccharides, low protein removal rate, and difficulty in completely removing organic solvents, and achieve uniform molecular weight and maintain physical and chemical properties , the effect of not easy to denature the target product

Active Publication Date: 2017-09-29
博德生物技术(德州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these methods, except the papain method, organic solvents are used, which are difficult to completely remove after deproteinization
Although the papain method is safe, it has the disadvantages of low protein removal rate and excessive polysaccharide loss rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 1,000g of fresh burdock root after cleaning and peeling, and beat the burdock root with a beater; add 2,500mL of pure water after beating, heat to 80°C, and perform homogenization during the heating process; after homogenization, use a peristaltic The pump was fed into the high-voltage pulsed electric field extraction equipment at 100mL / min for extraction, and the parameters were set to the electric field strength of 10kV / cm, and the number of pulses was 6; after the high-voltage pulsed electric field was extracted, it was centrifuged, and the supernatant was taken; Membrane filtration, discard the upper part of the membrane; the lower part of the membrane obtained by ultrafiltration is filtered using an ultrafiltration membrane with a molecular weight cut-off of 800Da until the density of the upper part of the membrane is 1.11g / mL, and the upper part of the membrane is collected; spray-dried to obtain burdock fructooligosaccharide 53.6g.

[0032] Carry out chemica...

Embodiment 2

[0034] Take 100g of dried burdock root slices, crush them through a 100-mesh sieve with a pulverizer, add 1,500mL of pure water, heat to 80°C, and perform homogenization during the heating process; after homogenization, use a peristaltic pump to feed the The high-voltage pulse electric field extraction equipment is used for extraction, and the parameters are set to electric field strength 20kV / cm, pulse number 5; after the high-voltage pulse electric field extraction is completed, centrifuge and take the supernatant; filter with an ultrafiltration membrane with a molecular weight cut-off of 7,000Da, and discard the membrane Upper part; the lower part of the membrane obtained by ultrafiltration is filtered using an ultrafiltration membrane with a molecular weight cut-off of 1,000Da until the density of the upper part of the membrane is 1.08g / mL, and the upper part of the membrane is collected; spray-dried to obtain 26.4g of burdock fructooligosaccharide.

[0035] Carry out chemica...

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Abstract

The invention provides a method for extracting burdock fructo-oligosaccharides from burdock roots, which mainly includes: extracting the treated burdock root material with a high-voltage pulse electric field, extracting the supernatant through centrifugal precipitation, and passing the supernatant twice The burdock fructooligosaccharide concentrate is obtained by ultrafiltration membrane filtration, and finally the concentrate is dried to obtain burdock fructooligosaccharide with relatively uniform molecular weight.

Description

technical field [0001] The invention relates to a method for extracting natural products from plants, in particular to a method for extracting burdock fructooligosaccharides from burdock roots. Background technique [0002] Burdock root is the root of Compositae plant burdock (Arctium lappa L.), and burdock is a kind of better medicinal and edible homologous plant. Burdock root is rich in oligosaccharides, called burdock fructooligosaccharides, burdock oligosaccharides, Burdock inulin and so on. According to reports, burdock fructooligosaccharides have the function of effectively lowering blood lipids and are a valuable natural product. [0003] It is known that there are many methods for the extraction of burdock fructooligosaccharides in burdock roots, such as direct water extraction (Hao Linhua, etc., Journal of Ocean University of China, May, 2004, the 3rd phase of volume 34), microwave extraction (Wang Hongxin, etc., Chinese patent application number 200910182291.X), ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 张硕陈学文
Owner 博德生物技术(德州)有限公司
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