Method for preparing super paramagnetic carrier of cross-linking immobilized lipase
A fat immobilization and superparamagnetic technology, applied in the field of food chemistry, can solve problems such as inability to adapt to lipase interface activation characteristics, functional defects, damage, etc., achieve good magnetic separation performance, and overcome the effect of lipase activity-dependent interface effects
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[0023] Example 1
[0024] (1) Nano magnetic microsphere Fe 3 O 4 Preparation:
[0025] FeCl 3 ·6H 2 O is dissolved in anhydrous ethylene glycol, prepared into a solution with a concentration of 0.13mmol / mL, and then press FeCl 3 ·6H 2 The mass ratio of O to the treatment agent is 1:6. Add the treatment agent. The treatment agent is sodium acetate and polyethylene glycol. After the treatment agent is added, the concentration of sodium acetate is 95mg / mL, and then transferred to the polytetrafluoroethylene coated with inert material. In the vinyl fluoride reactor, a constant temperature and ultrasonic reaction at 200℃ for 5h, after natural cooling, first wash with ethanol and deionized water for several times, then wash with deionized water, and then vacuum dry to obtain nano magnetic micro Ball Fe 3 O 4 ;
[0026] (2) Nano magnetic microsphere Fe 3 O 4 Package:
[0027] The nano magnetic microsphere Fe obtained in step (1) 3 O 4 Mix with 1mol / L NaOH solution until nano-magnetic micros...
Example Embodiment
[0031] Example 2
[0032] (1) Nano magnetic microsphere Fe 3 O 4 Preparation:
[0033] FeCl 3 ·6H 2 Dissolve O in anhydrous ethylene glycol, prepare a solution with a concentration of 0.1mmol / mL, and press FeCl 3 ·6H 2 The mass ratio of O to the treatment agent is 1:5. Add the treatment agent. The treatment agent is sodium acetate and polyethylene glycol. After the treatment agent is added, the concentration of sodium acetate is 120mg / mL, and then transferred to the polytetrafluoroethylene coated with inert material. In the vinyl fluoride reactor, a constant temperature ultrasonic reaction at 200°C for 6 hours, after natural cooling, first wash with ethanol and deionized water for several times, then wash with deionized water, and then vacuum dry to obtain nano magnetic micro Ball Fe 3 O 4 ;
[0034] (2) Nano magnetic microsphere Fe 3 O 4 Package:
[0035] The nano magnetic microsphere Fe obtained in step (1) 3 O 4 Mix with 1mol / L NaOH solution until nano-magnetic microsphere Fe is o...
Example Embodiment
[0039] Example 3
[0040] (1) Nano magnetic microsphere Fe 3 O 4 Preparation:
[0041] FeCl 3 ·6H 2 Dissolve O in anhydrous ethylene glycol, prepare a solution with a concentration of 0.15mmol / mL, and press FeCl 3 ·6H 2 The mass ratio of O to the treatment agent is 1:4. Add the treatment agent. The treatment agent is sodium acetate and polyethylene glycol. After the treatment agent is added, the concentration of sodium acetate is 80mg / mL, and then transferred to the polytetrafluoroethylene coated with inert material. In the vinyl fluoride reactor, a constant temperature ultrasonic reaction at 200°C for 4 hours, after natural cooling, first wash with ethanol and deionized water alternately for several times, then wash with deionized water, and then vacuum dry to obtain nano magnetic micro Ball Fe 3 O 4 ;
[0042] (2) Nano magnetic microsphere Fe 3 O 4 Package:
[0043] The nano magnetic microsphere Fe obtained in step (1) 3 O 4 Mix with 1mol / L NaOH solution until nano-magnetic microsp...
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