A method for detecting proteins based on graphene sensors
A sensor and protein technology, applied in the field of biochemical analysis and detection, can solve the problems of affecting accuracy, detection specificity, complex composition, etc., and achieve the effects of reducing resistance, reducing resistivity, and increasing carrier concentration
Active Publication Date: 2017-08-25
厦门烯成石墨烯科技有限公司
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Problems solved by technology
The invention can detect DNA helicase with high sensitivity, and the cost is low, but because graphene oxide is easy to self-aggregate in a complex environment, it will greatly affect the accuracy of detection; at the same time, due to the complex composition of clinical samples, the Proteins and other biomolecules will be adsorbed on the surface of nanomaterials to varying degrees, which will affect the specificity of detection
Therefore, the method is difficult to apply to the instance of clinical application
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[0023] In order to make the purpose, features and advantages of the present invention clearer, the specific implementation of the present invention will be described in more detail below in conjunction with the accompanying drawings and embodiments. In the following description, many specific details are set forth for the convenience of The invention is fully understood, but it can be practiced in many other ways than that described. Accordingly, the present invention is not limited to the specific implementations disclosed below.
[0024] A method for detecting proteins based on graphene sensors such as figure 1 shown, including the following steps:
[0025] (1) Make graphene sensor: as figure 2 As shown, a graphene film 2 is provided on a substrate 1, and electrodes 3 are provided at both ends of the graphene film 2;
[0026] (2) Pretreatment of the sample to be detected: adding a fluorescent dye to the sample to be detected to label the protein in the sample to obtain a...
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The invention discloses a method for detecting protein based on a graphene sensor, comprising the following steps: (1) making a graphene sensor: a graphene film is provided on a substrate, and electrodes are provided at both ends of the graphene film; (2) Pretreatment of the sample to be detected: adding a fluorescent dye to the sample to be detected to label the protein in the sample to obtain a fluorescent dye-protein complex; (3) detection of protein content in the sample: the pretreated to be detected When the sample is dropped on the surface of the graphene film, the fluorescence is quenched under the irradiation of ultraviolet light, and the resistance of the graphene film changes. content. The method of the invention can detect proteins with high sensitivity and high precision, and compared with the traditional fluorescence excitation detection method, the method is simple and the measurement speed is fast.
Description
technical field [0001] The invention relates to the technical field of biochemical analysis and detection, in particular to a method for detecting proteins based on a graphene sensor. Background technique [0002] Protein is an important matrix component of all cells and tissues in organisms, and an important bearer of life activities. It is closely related to nutrition, enzymes, viruses, immunity, genetics, etc. Therefore, it is very important to analyze the protein content. Significance, essential in disease diagnosis, biomedical research and nutritional analysis evaluation. At present, commonly used protein content detection methods include amino acid analysis, Kjeldahl method, absorbance photometry, colorimetry, ultraviolet spectrophotometry and so on. Fluorescence analysis is a protein content detection method developed in recent years, which has the characteristics of high sensitivity and wide linear range. The method of using the fluorescence method to determine the...
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IPC IPC(8): G01N21/64
Inventor 王振中
Owner 厦门烯成石墨烯科技有限公司