Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composite anti-biological inoculant containing alga matrix, and preparation method and use thereof

A biocontrol agent and substrate technology, applied in the field of compound biocontrol agent and preparation, can solve problems such as few reports, and achieve excellent bacteriostatic effect, strong inhibitory effect, and the effect of promoting plant growth.

Active Publication Date: 2015-11-18
LIAOCHENG UNIV
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biocontrol agents used to prevent and control the root rot of traditional Chinese medicinal materials are relatively rare at home and abroad. The domestically registered microbial pesticides are mainly used for wheat take-all, rice and wheat sheath blight, and fruit and vegetable wilt. Antifungal agents are used to prevent and control root rot, namely "Bai Kang" and "Root Rot", and they are both used for root rot of Panax notoginseng. It is rarely reported, so it deserves further study

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite anti-biological inoculant containing alga matrix, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]A kind of composite biocontrol bacterium agent containing seaweed substrate, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 2: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:1. Both Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 were preserved on May 29, 2014 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101. Among them, the preservation number of Bacillus subtilis 2-1 is: CGMCC No.9256, and the classification name is: Bacillus subtilis (Bacillus subtilis); the preservation number of Bacillus amyloliquefaciens 9-2 is: CGMCCNo.9257, and the classification ...

Embodiment 2

[0052] A kind of composite biocontrol bacterium agent containing seaweed matrix, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 8: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:2.

[0053] A preparation method of a composite biocontrol agent containing seaweed matrix, comprising the following steps:

[0054] 1) Inoculation fermentation

[0055] a. Under aseptic operation conditions, use a pipette gun to transfer the biocontrol strains 2-1 and 9-2 preserved in glycerol tubes according to different dosages to the glass plates equipped with the first medium, and incubate at 37°C for 18 hours Activation of bacteria;

[0056] The formula of the first medium is: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar 20g / L, and the pH value is 7.5;

[0057] b. Inoculate the a...

Embodiment 3

[0068] A kind of composite biocontrol bacterium agent containing seaweed matrix, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 4: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:3.

[0069] A preparation method of a composite biocontrol agent containing seaweed matrix, comprising the following steps:

[0070] 1) Inoculation fermentation

[0071] a. Under aseptic operation conditions, inoculate the biocontrol strains 2-1 and 9-2 preserved in glycerol tubes according to different dosages on the glass plates containing the first medium with a pipette gun, and incubate at 37°C for 18 hours Activation of bacteria;

[0072] The formula of the first medium is: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar 20g / L, and the pH value is 7.5;

[0073] b. Inoculate the activ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composite anti-biological inoculant containing an alga matrix, and a preparation method and a use thereof. The anti-biological inoculant comprises alga residues, Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2, wherein a ratio of the alga residues to a bacillus fermentation solution is 2-8:1 (w / v), and a ratio of the Bacillus subtilis 2-1 to the Bacillus amyloliquefaciens 9-2 is 1:1-5. The preparation method comprises the following steps: carrying out inoculation fermentation, preparing the anti-biological inoculant containing the alga matrix, drying, and packaging. The composite anti-biological inoculant has strong inhibition effects on root rot pathogenic bacteria of Salvia miltiorrhiza, Rhizoma Arisaematis and other traditional Chinese medicinal materials, has a very good control effect on vegetable and crop root rot, and has a better bacteriostasis effect than single strains.

Description

technical field [0001] The invention belongs to the field of biocontrol fungi, in particular to a compound biocontrol fungus containing seaweed matrix, its preparation method and application. Background technique [0002] Biocontrol Bacillus has many types, wide distribution, broad antibacterial spectrum, and obvious disease resistance and yield increase effect, which is superior to other antagonistic microorganisms. At present, there have been reports of its successful use in plant disease control at home and abroad. There are 14 kinds of microbial pesticides that have been registered in my country to prevent and control soil-borne diseases, of which 6 kinds use Bacillus as the biocontrol bacteria, which are mainly used for the control of rice and wheat sheath blight. In recent years, they have been rapidly applied to tomato Various crop and / or fruit and vegetable diseases such as bacterial wilt, cotton verticillium wilt, pepper wilt, watermelon wilt, tobacco black shank, ri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/00A01P3/00
Inventor 陈芳董惠钧陈坤郭尚敬韩军周莹
Owner LIAOCHENG UNIV
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com