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Method for extracting, separating and purifying three kinds of coumarin from dahurian angelica root

A technology for separation and purification of coumarin, applied in the direction of organic chemistry, etc., can solve the problems of solvent system selection difficulties, lack of theoretical guidance, cumbersome operation process, etc., and achieve the effect of improving extraction efficiency, simple method operation, and high purity

Inactive Publication Date: 2015-11-18
LIAOCHENG UNIV
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Problems solved by technology

According to literature reports, the methods for separating and purifying Angelica dahurica coumarin mainly include repeated silica gel column chromatography and high-speed countercurrent chromatography. The operation process of silica gel column chromatography is cumbersome, the recovery rate is low, and chloroform, benzene, etc. are often used. organic solvents; the choice of solvent system in high-speed countercurrent chromatography is difficult and lacks theoretical guidance

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  • Method for extracting, separating and purifying three kinds of coumarin from dahurian angelica root
  • Method for extracting, separating and purifying three kinds of coumarin from dahurian angelica root
  • Method for extracting, separating and purifying three kinds of coumarin from dahurian angelica root

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Embodiment

[0033] Embodiment: the method for extracting, separating and purifying 3 kinds of coumarins from Angelica dahurica, its steps are:

[0034] (1) Extraction of Angelica dahurica coumarin: Pulverize the medicinal material of Angelica dahurica, heat and extract 3 times with 10 times the amount of petroleum ether, 2 hours each time, filter, combine the extracts, and concentrate under reduced pressure to obtain a crude extract.

[0035] (2) Separation and analysis of Angelica dahurica coumarin: use analytical high performance liquid chromatography to separate and analyze the composition of the crude extract, and the chromatographic column is C 18 Column (250mm×4.6mmI.D., 5μm), the mobile phase is methanol-water (67:33, V / V); the flow rate is 1.0mL / min, the detection wavelength is 220nm, and the column temperature is room temperature.

[0036] (3) Separation and purification of Angelica dahurica coumarin: The components in the crude extract were separated and purified by semi-prepara...

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Abstract

The invention relates to a method for extracting, separating and purifying three kinds of coumarin from dahurian angelica root. The method is characterized by using the medicinal material dahurian angelica root as a raw material and comprises the following steps: (1) extraction of coumarin in dahurian angelica root; (2) separation analysis of coumarin in dahurian angelica root; (3) separation and purification of coumarin in dahurian angelica root; (4) compound purity detection and structure identification. The method has the advantages that three high-purity components are obtained by using semi-preparative high performance liquid chromatography for separation and purification and using methanol-water as a mobile phase and are identified respectively to be imperatorin, oxidized imperatorin and isoimperatorin; the process is environment-friendly in course, does not have severe harm to the environment and is low in comprehensive cost.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for extracting, separating and purifying three kinds of coumarins from Angelica dahurica. Background technique [0002] Angelica dahurica was first recorded in "Shen Nong's Materia Medica". It is warm in nature and pungent in taste. It has the functions of dispersing wind and dehumidification, clearing orifices, relieving pain, reducing swelling and discharging pus. , leucorrhea and sore swelling and pain embolism. In addition, Angelica dahurica is also an essential raw material in the production of various health products, cosmetics, spices and seasonings. The chemical composition of Angelica dahurica is complex, mainly containing coumarin and volatile oil. Pharmacological studies have shown that coumarin is the main active ingredient of Angelica dahurica, among which imperatorin, isoimperatorin and oxyprecurin are relatively high in content. These three s...

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Application Information

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IPC IPC(8): C07D493/04
CPCC07D493/04
Inventor 李爱峰于琳琳柳仁民孙爱玲
Owner LIAOCHENG UNIV
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