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A kind of nano-BT compound insecticide and its preparation method and application

An insecticide and nanotechnology, which is applied in the field of nano-Bt compound insecticide and its preparation, can solve the problems of failing to meet control requirements, reducing insecticidal effect, and short duration of field efficacy, and achieving excellent insecticidal effect. The effect of activity, reducing dosage, and improving drug stability

Active Publication Date: 2018-06-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some problems have also been found in the agricultural practice of using Bacillus thuringiensis insecticides, especially the short duration of field efficacy, which is easily affected by field environmental factors such as sunlight, temperature and humidity, resulting in its insecticidal effect. The effect is reduced, and the requirements of stable and long-term prevention and control cannot be met

Method used

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  • A kind of nano-BT compound insecticide and its preparation method and application
  • A kind of nano-BT compound insecticide and its preparation method and application
  • A kind of nano-BT compound insecticide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of the compound insecticide of Bacillus thuringiensis YBT-1520 and nano-scale birnessite is as follows:

[0025] The dry powder of Bacillus thuringiensis YBT-1520 used in the examples of the present invention can be prepared in a conventional way, or by the following method:

[0026] (1) Preparation of Bacillus thuringiensis YBT-1520: Transfer the isolated and purified Bacillus thuringiensis YBT-1520 to LB solid medium for 24 hours and observe the colony shape; then, pick a single colony and carry out Gram test Staining observation. LB solid medium formula: protein building 10g; beef extract 5g; sodium chloride 5g; agar 2.5g, distilled water to 1000mL, adjust the pH value to 7.0-7.2.

[0027] LB liquid medium formula: protein building 10g; beef extract 5g; sodium chloride 5g; distilled water to 1000mL, adjust the pH value to 7.0-7.2.

[0028] (2) Strain activation: Streak inoculation of YBT-1520 onto solid medium LB, culture at 28°C for 12 hours, pick ...

Embodiment 2

[0041] Cotton bollworm activity experiment

[0042] The Bacillus thuringiensis YBT-1520 prepared in Example 1 and the nano-Bt compound insecticide prepared in Example 1, after ultraviolet treatment (280-300 μ w / cm2 , 365nm, 30cm), biological potency under different UV irradiation time (15min, 30min, 60min).

[0043] Attenuation experiment of one month under normal temperature (20-25 ℃) indoor conditions, on the 15th day, on the 30th day, measure the biological potency of Bacillus thuringiensis YBT-1520 and the nano-Bt compound insecticide prepared in Example 1 .

[0044] test results

[0045] Table 1 The poisonous effect of traditional Bt insecticides and nano-Bt compound insecticides on cotton bollworm under ultraviolet light irradiation

[0046]

[0047]

[0048] The results showed that after mixing and compounding nanomaterials and Bt, the stability of the compound was better after ultraviolet irradiation, so the poisonous effect on cotton bollworm was better.

[0...

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Abstract

The invention discloses a Nano Bt compound insecticide and a preparation method and application. The preparation method comprises: mixing bacillus thuringiensis Bt with nanoscale birnessite; adding distilled water and uniformly mixing the mixture; placing the mixture in a table at 37 DEG C and rocking the table at 200 r / min; and combining the mixture for 2 hours to obtain the nano Bt compound insecticide. Compared with the prior art, by adopting a nano material and Bt to compound the insecticide, the insecticide has the advantages of being small in particle size, large in specific surface area, high in surface energy and high in efficiency and low in toxicity, can improve the drug stability, increase the tolerance of insecticidal crystal protein on a poor environment and greatly reduce the degradation rate, so that the insecticidal activity can be enhanced. The nano Bt compound insecticide has excellent insecticidal activity on injurious insects, and finally, the Bt preparation is more effectively utilized. Meanwhile, the use level of the effective components is greatly reduced, so that the cost is lowered.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a nano-Bt compound insecticide, a preparation method and an application. Background technique [0002] People depend on food, and the importance of agriculture is self-evident. However, from ancient times to the present, a large amount of food will be lost every day due to pests and diseases. Therefore, controlling pests and diseases is a key way to increase food production. Nowadays, chemical pesticides are widely used for prevention and control. Although the effect of this method is remarkable, the pollution to the environment cannot be underestimated. Therefore, people begin to develop novel high-efficiency pesticide variety medicine that does not pollute the environment. Biopesticides are derived from the organism itself or its metabolites. They have good safety performance and do not pollute the environment. They are environmentally friendly pesticides. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P7/04A01N59/16
Inventor 李林向雪梅
Owner HUAZHONG AGRI UNIV