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Live vaccine and preparation method thereof

A technology for vaccines and immune adjuvants, applied in the field of veterinary vaccines and their preparation, can solve the problems such as the inability to rapidly induce the production of high-titer antibodies, the antibody titers being lower than the normal value, and the short antibody protection period, etc. Huge, solve vaccine side effects, low cost effect

Active Publication Date: 2015-11-25
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, RHD inactivated vaccine is used clinically for prevention, but there are many defects: high titer antibodies cannot be induced quickly after injection, and some rabbits have been infected with rabbit plague virus before producing protective antibodies; the protection period of antibodies is short , the antibody level drops rapidly. If the second immunization is not timely, the antibody titer will soon be lower than the normal value; the storage conditions of the vaccine are relatively strict, and improper storage will easily lead to a decrease in the vaccine titer

Method used

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  • Live vaccine and preparation method thereof
  • Live vaccine and preparation method thereof
  • Live vaccine and preparation method thereof

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preparation example Construction

[0024] Aiming at the problem of developing new vaccines, the present invention provides a veterinary vaccine and a preparation method thereof.

[0025] Pine pollen polysaccharides are composed of monosaccharides such as mannose, glucuronic acid, rhamnose, glucose, galactose, arabinose and fucose. Modern studies have proved that pine pollen polysaccharide is one of the nutrients that maintain the special effects of pine pollen, and has very high biological activity. After the pine pollen is broken, the amount of polysaccharide extracted is significantly increased. In the present invention, fresh pine pollen in the Taishan area is collected in spring, the crude polysaccharide is extracted by water extraction and alcohol precipitation after sieving and breaking the wall, and two methods of pepsin and Sevage reagent are used in the extraction process to remove protein, which ensures the higher purity of the crude polysaccharide.

[0026] A variety of plant polysaccharides can not only ...

Embodiment 1

[0029] Example 1 Vaccine 1

[0030] A veterinary vaccine. The vaccine consists of an immune adjuvant and an antigen aqueous solution with a mass ratio of 4:96.

[0031] The antigen aqueous solution is mixed with rabbit hemorrhagic disease virus material with a hemagglutination titer of 12log2 and physiological saline at a mass ratio of 1:10, and the antigen solution is obtained after thorough homogenization.

[0032] The immune adjuvant is composed of the following raw materials in parts by mass: 5 parts of Taishan pine pollen polysaccharide, 10 parts of propolis, 5 parts of aloe polysaccharide, 8 parts of raspberry polysaccharide, 6 parts of Laojun's beard polysaccharide, 9 parts of Dawanhua polysaccharide, hydrophilicity 10 parts of surfactant, 0.1 part of polymer micelle substance and 40 parts of water.

[0033] Among them, the hydrophilic surfactant is a mixture of Span 80 and Pluronic 121; the mass ratio of Span 80 and Pluronic 121 is 1 part of Span 80 and 3 parts of Pluronic 121...

Embodiment 2

[0043] Example 2 Adjuvant 2

[0044] A veterinary vaccine. The vaccine is composed of an immune adjuvant and an aqueous antigen solution with a mass ratio of 11:89.

[0045] The antigen aqueous solution is mixed with rabbit hemorrhagic disease virus material with a hemagglutination titer of 11log2 and physiological saline in a mass ratio of 1:10, and the antigen solution is obtained after thorough homogenization.

[0046] The immune adjuvant is composed of the following raw materials in parts by mass: 30 parts of Taishan pine pollen polysaccharide, 5 parts of propolis, 10 parts of aloe polysaccharides, 14 parts of raspberry polysaccharides, 3 parts of Laojun's beard polysaccharides, 4 parts of Dawanhua polysaccharides, hydrophilic 0.1 part of sexual surfactant, 10 parts of polymer micelle substance and 90 parts of water.

[0047] Among them, the hydrophilic surfactant is a mixture of Span 80 and Pluronic 121; the mass parts ratio of Span 80 and Pluronic 121 is 2.5 parts for Span 80 an...

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Abstract

The invention discloses a live vaccine and a preparation method thereof. The live vaccine is composed of immune adjuvant and an antigen aqueous solution according to the mass ratio of 4-11 to 89-96. The live vaccine and the preparation method have the advantages of being simple in preparation process, low in cost, simple in component, stable in dosage form, easy to store and inject, convenient to use, good in use effect and free of toxic and side effects, having great development and utilization potential in the animal husbandry, and having wide application prospects.

Description

Technical field [0001] The invention relates to a vaccine adjuvant, in particular to a veterinary vaccine and a preparation method thereof. Background technique [0002] Rabbit plague, also known as rabbit viral hemorrhagic disease (RHD), is an acute and highly contagious infectious disease in rabbits caused by rabbit viral hemorrhagic disease virus (RHDV). All kinds of rabbits are susceptible throughout the year. The disease is characterized by respiratory system bleeding, liver necrosis, parenchymal organ edema, congestion and hemorrhagic changes. Since it was first discovered in Wuxi, Jiangsu Province, my country in 1984, it quickly spread to many provinces and cities across the country. So far North Korea, South Korea, Italy, France, Germany, the Czech Republic, the United States and New Zealand and other countries have epidemics of the disease. The disease has a short incubation period, rapid onset, short course, rapid spread, and extremely high morbidity and mortality. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61K9/08A61P31/14
Inventor 朱瑞良胡莉萍魏凯彭军钟世勋
Owner SHANDONG AGRICULTURAL UNIVERSITY