Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for detecting p-nitrophenol based on molecularly imprinted ratiometric fluorescent probe

A p-nitrophenol, molecular imprinting technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of reducing the selectivity of the detection process

Active Publication Date: 2017-09-29
TIANJIN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This probe based on a single fluorescence intensity change is not only susceptible to the influence of the detection substrate, photobleaching and other shortcomings, but also may be interfered by fluorescent quenchers such as heavy metal ions and active oxygen radicals at the same time, which reduces the detection process. selectivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for detecting p-nitrophenol based on molecularly imprinted ratiometric fluorescent probe
  • A method for detecting p-nitrophenol based on molecularly imprinted ratiometric fluorescent probe
  • A method for detecting p-nitrophenol based on molecularly imprinted ratiometric fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for detecting p-nitrophenol based on molecularly imprinted ratiometric fluorescent probes, the steps are as follows:

[0026] (1) Preparation of fluorescent silica nanoparticles coated with quantum dots;

[0027] Fluorescent silica nanoparticles coated with quantum dots are prepared by adopting the methods of published Chinese patents (authorized notification numbers: CN102757790B and CN1306002C).

[0028] (2) Preparation of siloxane functionalized carbon dots;

[0029] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0030] (3) Preparation of core-shell dual-emission fluorescent silica nanoparticles;

[0031] Take 5 mg of fluorescent silica nanoparticles coated with quantum dots obtained in step (1) and ultrasonically disperse them in 50 mL of anhydrous toluene, add 5 μL of siloxane functionalized carbon dots obtained in step (2), stir evenly, and heat to Reflux te...

Embodiment 2

[0037] (1) Preparation of fluorescent silica nanoparticles coated with quantum dots;

[0038] Fluorescent silica nanoparticles coated with quantum dots are prepared by adopting the methods of published Chinese patents (authorized notification numbers: CN102757790B and CN1306002C).

[0039] (2) Preparation of siloxane functionalized carbon dots;

[0040] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0041] (3) Preparation of core-shell dual-emission fluorescent silica nanoparticles;

[0042]Take 5 mg of fluorescent silica nanoparticles coated with quantum dots obtained in step (1) and ultrasonically disperse them in 100 mL of anhydrous toluene, add 15 μL of the siloxane functionalized carbon dots obtained in step (2), stir evenly, and heat to Reflux temperature, after 16 hours of reaction, the product was centrifuged to obtain 5.9 mg of solid powder of core-shell dual-emission flu...

Embodiment 3

[0048] (1) Preparation of fluorescent silica nanoparticles coated with quantum dots;

[0049] Fluorescent silica nanoparticles coated with quantum dots are prepared by adopting the methods of published Chinese patents (authorized notification numbers: CN102757790B and CN1306002C).

[0050] (2) Preparation of siloxane functionalized carbon dots;

[0051] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0052] (3) Preparation of core-shell dual-emission fluorescent silica nanoparticles;

[0053] Take 5 mg of fluorescent silica nanoparticles coated with quantum dots obtained in step (1) and ultrasonically disperse them in 75 mL of anhydrous toluene, add 10 μL of siloxane functionalized carbon dots obtained in step (2), stir evenly, and heat to Reflux temperature, after 12 hours of reaction, the product was centrifuged to obtain 5.8 mg of solid powder of core-shell dual-emission fluores...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for detecting p-nitrophenol based on molecular imprinting ratio fluorescent probe. Molecularly imprinted ratiometric fluorescent probes are composed of core-shell dual-emission molecularly imprinted silica nanoparticles; dual-emission ratiometric fluorescent probes are prepared by using silica nanoparticles coated with quantum dots and siloxane functionalized carbon dots Nanoparticles, and then grow a silicon dioxide layer containing imprinted molecule p-nitrophenol on the surface to obtain molecularly imprinted double fluorescent composite nanoparticles. In this structure, the quantum dots located inside the silica nanoparticle core serve as a reference fluorescence signal, and the carbon dots in the outer molecular imprinted layer serve as a response fluorescence signal for the selective recognition of p-nitrophenol. It fully utilizes the advantages of molecular imprinting such as anti-interference, high selectivity, and high sensitivity, overcomes the traditional single fluorescent signal analysis method that is easily affected by factors such as detection substrate concentration and external environment, and has great potential in the fields of environmental pollutant monitoring and control. great significance.

Description

technical field [0001] The invention relates to a fluorescence analysis and detection method for p-nitrophenol, in particular to a method for detecting p-nitrophenol based on a molecular imprinting ratio fluorescent probe. Background technique [0002] The pollution of phenol and phenolic compounds to water has attracted widespread attention, and my country has listed it as one of the pollutants that need to be controlled first in water. Among them, p-nitrophenol, as an important chemical raw material, is widely used in fine chemical industries such as explosives, dyes, and pharmaceutical intermediates, and is a class of highly toxic and refractory compounds. Because of its extensive use, a large amount of wastewater is produced, which not only pollutes the environment but also endangers biological safety. In order to ensure human health, the concentration of p-nitrophenol in water should be lower than 70 μg / L, so it is very important to accurately detect the content of p-n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 苏荣欣高召黄仁亮齐崴王梦凡
Owner TIANJIN UNIV