Method for determining total alkaloid content in black nightshade

A technology for total alkaloids and solanine, which is applied in the measurement of color/spectral properties, etc., can solve the problems of tediousness, high cost of reagents, and high technical requirements for operation

Inactive Publication Date: 2015-12-02
JIAXING UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is sensitive and accurate to the content determination of monomeric compounds, but when used for the determination of total alkaloid content, it requires high operating

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining total alkaloid content in black nightshade
  • Method for determining total alkaloid content in black nightshade
  • Method for determining total alkaloid content in black nightshade

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 adopts the present invention to the Solanum nigrum medical material through ultrasonic extraction total alkaloid content determination

[0050] Solanum nigrum decoction pieces were dried at 60°C, crushed and passed through a 14-mesh sieve. Accurately weigh 10.0 g of Solanum nigrum powder, place it in a conical flask, add 150 mL of a mixed solvent of 2 mol / L hydrochloric acid: 90% ethanol (volume ratio 1: 4), ultrasonically extract for 35 min at 40 ° C, extract twice, and extract Recover the ethanol under reduced pressure to an alcohol-free extract, dissolve the extract with 10% hydrochloric acid, sonicate for 20 minutes to aid dissolution, remove the precipitate by suction filtration, and dilute to 50 mL with 10% hydrochloric acid. Accurately draw 10ml from it, adjust the pH to 10-11 with 10% NaOH, add chloroform to extract 3 times, collect the chloroform solution, and dilute to 25ml to obtain the test solution.

[0051] Accurately weigh 5.00 mg of solanin...

Embodiment 2

[0053] Embodiment 2 adopts the present invention to the determination of the content of the total alkaloids extracted from Solanum solanum medicinal materials

[0054] Solanum nigrum decoction pieces were dried at 60°C, crushed and passed through a 14-mesh sieve. Accurately weigh 10.0 g of Solanum nigrum powder, place it in a round-bottomed flask, add 150 mL of a mixed solvent of 2 mol / L hydrochloric acid: 90% ethanol (1:4), cover and airtightly soak for 8 h, heat and reflux in a water bath at 80°C for 2 h, extract Twice, combine the extracts, recover the ethanol from the extracts under reduced pressure to obtain an alcohol-free extract, dissolve the extract with 10% hydrochloric acid, sonicate for 20 minutes to help dissolve, remove the precipitate by suction filtration, and dilute to a 50mL volumetric flask with 10% hydrochloric acid. Precisely draw 10mL of acidic water, adjust the pH to 10-11 with 10% NaOH, transfer to a separatory funnel, add chloroform for extraction 3 ti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for determining total alkaloid content in black nightshade with an ultraviolet spectrophotometer by adopting an acid dye colorimetric method. The method is characterized in that by adopting black nightshade alkali as a reference substance, a reference substance solution is prepared by using chloroform; ultrasonic extraction of total alkaloid in black nightshade is carried out by adopting an acid-alcohol mixed solvent to prepare a test solution; and chromogenic conditions of the acid dye colorimetric method are as follows: pH is equal to 3.6, 3.5 mL of a buffer solution and 2.0 mL of a bromocresol green solution are used, extraction is carried out three times with 10 mL of chloroform, and standing is kept for 30 min. The detection wavelength is 243 nm, and content determination is carried out according to the absorbance. The total alkaloid of the black nightshade is in a linear relationship within a range of 5-30 mu g/mL, the average value of recovery rate is 99.7%, and RSD is equal to 1.4% (n is equal to 6). The method for determining the total alkaloid content in black nightshade disclosed by the invention is simple and convenient to operate, low in cost, high in recovery rate and good in reproducibility, and can be used for determining the total alkaloid content in the black nightshade.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and relates to a method for determining the content of total alkaloids in nightshade nightshade. Background technique [0002] Solanum nigrum is the dry aerial part of Solanum nigrum, a plant of the Solanaceae family. It is cold in nature, bitter in taste, slightly sweet, slightly poisonous, and returns to the lung and stomach meridians. The pharmacologically active components of nightshade are mainly alkaloids, including solanine, solanine, solanine, solanine, and solanine. A few literatures have reported the content determination methods of Solanum solanum and its preparations, all of which use solanine and solanine as reference substances, and use high performance liquid chromatography or mass spectrometry for determination. This method is sensitive and accurate to the content determination of monomeric compounds, but when used for the determination of total alk...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/33
Inventor 黄越燕高广春吴世平
Owner JIAXING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap