Method for extracting luteolin from peanut shells

A technology of luteolin and peanut shells, which is applied in the field of extraction in the field of biotechnology, can solve the problems of ineffective utilization and waste of peanut shells, and achieve the effects of facilitating separation, improving extraction rate, and maintaining osmotic pressure

Inactive Publication Date: 2015-12-09
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Peanut shells are very common in life. Most of the time, they are treated as garbage, some are direc...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for extracting luteolin from peanut shells specifically includes the following steps:

[0041] 1) Soaking treatment: Put the peanut shells into a woven bag, tie the mouth, and put it in the pool, fill the pool with water, and press heavy objects on the pool, soak for 15 hours;

[0042] 2) Raw material processing: pulverize the peanut shells obtained in step 1), add purified water equivalent to 3 times the weight of the raw materials, and heat to 60°C for 5 minutes to obtain a paste-like slurry;

[0043] 3) Enzymatic hydrolysis: adjust the pH of the paste slurry obtained in step 2) to 4, add 0.1% by weight of the paste slurry with a pectin-cellulose complex enzyme with an activity of 30,000 units / g and 0.001 parts by weight of manna Enzymatic hydrolysis with alcohol for 60 minutes to obtain enzymatic hydrolysis solution;

[0044] 4) Degreasing: add 3 times the weight of purified water to the enzymatic hydrolysate obtained in step 3), heat it to 70°C for 60 minutes, an...

Embodiment 2

[0049] A method for extracting luteolin from peanut shells specifically includes the following steps:

[0050] 1) Soaking treatment: Put the peanut shells into a woven bag, tie the mouth, and put it in the pool. The pool is filled with water, and heavy objects are placed on the pool for 20 hours;

[0051] 2) Raw material processing: crush the peanut shells obtained in step 1), add purified water equivalent to 3 times the weight of the raw materials, and heat to 50°C for 10 minutes to obtain a paste-like slurry;

[0052] 3) Enzymatic hydrolysis: adjust the pH of the paste slurry obtained in step 2) to 5, add 0.05% by weight of the paste slurry with a pectin-cellulose complex enzyme with an activity of 10,000 active units / g and 0.001 parts by weight of manna Enzymatic hydrolysis with alcohol for 40 minutes to obtain enzymatic hydrolysis solution;

[0053] 4) Degreasing: add 3 times the weight of purified water to the enzymatic hydrolysate obtained in step 3), and heat to 60°C for 60 m...

Embodiment 3

[0058] A method for extracting luteolin from peanut shells specifically includes the following steps:

[0059] 1) Soaking treatment: Put the peanut shells into a woven bag, tie the mouth, and put it in a pool. Fill the pool with water, and press a heavy object on the pool for 24 hours;

[0060] 2) Raw material processing: crush the peanut shells obtained in step 1), add purified water equivalent to twice the weight of the raw materials, and heat to 55°C for 10 minutes to obtain a paste-like slurry;

[0061] 3) Enzymatic hydrolysis: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 active units / g of pectin-cellulose complex enzyme and 0.001 parts by weight of manna Enzymatic hydrolysis with alcohol for 50 minutes to obtain enzymatic hydrolysis solution;

[0062] 4) Degreasing: add 2 times the weight of purified water to the enzymatic hydrolysate obtained in step 3), and heat to 65°C for 100 minutes to extra...

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PUM

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Abstract

The invention discloses a method for extracting luteolin from peanut shells. The method comprises the following steps of steeping treatment, raw material treatment, enzymolysis, deoiling, alkaline extraction, extraction and recrystallization. According to the method, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.

Description

Technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting luteolin from peanut shells. Background technique [0002] Peanut shell is the shell of peanut. The peanut fruit is a pod with cocoon, bead and hockey stick shapes. The color of the shell is mostly yellow-white, but also yellow-brown, brown or yellow, which is related to the variety and soil quality of peanuts. [0003] Peanut shells are very common in life. Most of the time, they are treated as garbage. Some are directly burned, and some are even thrown away. Failure to use the peanut shells is very wasteful. Plants are all treasures, and scientific treatment will bring more benefits to people. Peanut shell contains nearly 60% crude fiber, ranking first in roughage. The dry matter of peanut shell nutrients accounts for 90.3%, of which crude protein is 4.8-7.2%, crude fat is 1-1.1%, crude cellulose is 65.7-79.3%, hemicellulose is 10.1%, an...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 卢照凯陆美珍谢冬养赵明东钟德品梁小珍周晓青
Owner 桂林三宝生物科技有限公司
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