Bacterium strain capable of producing haematochrome and method for preparing haematochrome

A technology for red pigments and strains, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effects of simple equipment, easy extraction, and reduced extraction costs

Inactive Publication Date: 2015-12-09
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report on the production of bacterial red pigment, and bacteria have faster metabolism than fungi, which is a potential red pigment-producing strain.

Method used

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  • Bacterium strain capable of producing haematochrome and method for preparing haematochrome
  • Bacterium strain capable of producing haematochrome and method for preparing haematochrome
  • Bacterium strain capable of producing haematochrome and method for preparing haematochrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Described bacterial strain adopts following method to prepare:

[0054] (1) Separation of strains:

[0055] Collect the soil, weigh the soil sample and put it into the instrument, then add sterile water, the mass volume ratio of the soil sample to the sterile water is 1:8, shake at a speed of 140r / min for 25min, let it stand for 25min and then dilute it 10 times gradiently, draw 200uL of diluent, spread on Gaoshi No. 1 solid medium, beef extract peptone and PDA medium respectively, and incubate at a constant temperature of 26°C for 1-5d; the soil is dry soil under a weak light environment, which can be sandy Soil, generally choose the soil under the shade of trees, with no obvious moisture on the surface.

[0056] Among them, the formula of Gaoshi No. 1 solid medium is: 19 parts of soluble starch, KNO 3 0.8 parts, NaCl 0.4 parts, K 2 HPO 4 ·3H 2 O0.4 parts, MgSO 4 ·7H 2 O0.4 parts, FeSO 4 ·7H 2 O0.008 parts, 17 parts of agar, 0.98L of distilled water, pH7.1;

...

Embodiment 2

[0066] Described bacterial strain adopts following method to prepare:

[0067] (1) Separation of strains:

[0068] Collect the soil, weigh the soil sample and put it into the instrument, then add sterile water, the mass volume ratio of the soil sample and sterile water is 1:9, shake at a speed of 160r / min for 25min, let it stand for 35min and then dilute it 9 times gradiently, draw 195uL of the dilution solution is spread on Gaoshi No. 1 solid medium, beef extract peptone and PDA medium respectively, and cultured at a constant temperature of 29°C for 1-5d; the soil is dry soil under a weak light environment, which can be sandy Soil, generally choose the soil under the shade of trees, with no obvious moisture on the surface.

[0069] Among them, the formula of Gaoshi No. 1 solid medium is: 21 parts of soluble starch, KNO 3 1.2 parts, NaCl 0.6 parts, K 2 HPO 4 ·3H 2 O0.6 parts, MgSO 4 ·7H 2 O0.6 parts, FeSO 4 ·7H 2 O0.012 parts, 19 parts of agar, 1.2L of distilled water...

Embodiment 3

[0079] Described bacterial strain adopts following method to prepare:

[0080] (1) Separation of strains:

[0081] Collect the soil, weigh the soil sample and put it into the instrument, then add sterile water, the mass volume ratio of soil sample to sterile water is 1:8-9, shake at 140-160r / min for 25-35min, let stand for 25- After 35 minutes, 11-fold gradient dilution was taken, and 205uL of the diluted solution was drawn, and spread on Gaoshi No. 1 solid medium, beef extract peptone and PDA medium respectively, and incubated at a constant temperature of 26-29°C for 1-5d; the soil was weakly illuminated The dry soil in the environment can be sandy soil. Generally, the soil under the shade of trees and without obvious moisture on the surface is selected.

[0082] Among them, the formula of Gaoshi No. 1 solid medium is: 20 parts of soluble starch, KNO 3 1.1 parts, NaCl 0.55 parts, K 2 HPO 4 ·3H 2 O0.5 parts, MgSO 4 ·7H 2 O0.5 parts, FeSO 4 ·7H 2 O0.009 parts, 18 parts...

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Abstract

The invention belongs to the biotechnology field of biochemical industry and colorants, and particularly relates to a bacterium strain capable of producing haematochrome and a method for preparing the haematochrome. A bacterium XF 105 which is obtained through screening in soil and can produce the haematochrome is adopted as the bacterium strain, the bacterium strain is named as Paenibacillus agaridevorans XF 105 and preserved in China Center for Type Culture Collection, the preservation number is CCTCC:M2014644, and the preservation date is December 11, 2014, and the gene sequence of the bacterium strain is shown as SEQ ID No.1. The haematochrome can be stably produced and is natural in hue, the bacterium strain is easy to preserve, the preservation time is long, the survival rate is high, the bacterium strain can still survive after being preserved at a 4-degree bevel, and the extraction method of the haematochrome is low in cost.

Description

technical field [0001] The invention belongs to the field of biochemical engineering and colorant biotechnology, and in particular relates to a red pigment-producing bacterial strain and a method for preparing red pigment. Background technique [0002] Now commonly used food coloring includes two categories: natural coloring and synthetic coloring. Natural pigments come from natural products, mainly extracted from plant tissues, and also include some pigments from animals and microorganisms. Synthetic pigments refer to organic pigments prepared by artificial chemical synthesis methods, mainly made of aniline dyes separated from coal tar as raw materials. Synthetic pigments have the risk of carcinogenicity and teratogenicity. With the increase of carcinogenic incidents of synthetic pigments, the safety of pigments has attracted people's attention. At the same time, the rapid development of the food industry has also increased the market demand for pigments. As one of the th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C09B61/00C12R1/01
Inventor 左勇傅彬
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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